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J. Biol. Chem., Vol. 263, Issue 16, 7672-7677, 06, 1988
N Kaneda, H Ichinose, K Kobayashi, K Oka, F Kishi, A Nakazawa, Y Kurosawa, K Fujita and T Nagatsu
Department of Biochemistry, Nagoya University School of Medicine, Japan.
Phenylethanolamine N-methyltransferase (PNMT; EC 2.1.1.28) catalyzes the synthesis of epinephrine from norepinephrine, the last step of catecholamine biosynthesis. To isolate a cDNA clone for human PNMT, we first isolated a cDNA clone for bovine adrenal medulla PNMT using mixed oligodeoxyribonucleotide probes whose synthesis was based on the partial amino acid sequence of tryptic peptides from the bovine enzyme. By screening a bovine adrenal medulla cDNA library, a cDNA clone with an insert of about 200 base pairs (bp) was isolated. This clone consisted of 84 bp of carboxyl-terminal coding region, which contained amino acid sequences corresponding to two tryptic peptides, and about 100 bp of 3'-untranslated region. Using this cDNA fragment as the probe, we screened a human pheochromocytoma cDNA library and isolated a cDNA clone with an insert of about 1.0 kilobase pairs, which contained the complete coding region of the enzyme. Northern blot analysis of human pheochromocytoma poly(A)+ RNA using this cDNA insert as the probe showed a single RNA species of about 1,000 nucleotides, suggesting that this clone is a full-length cDNA. Determination of the nucleotide sequence revealed that human PNMT consists of 282-amino acid residues with a predicted molecular weight of 30,853, including initial methionine. The amino acid sequence of the human PNMT was highly homologous (88%) to that of the bovine enzyme. Chromosomal assignment of the gene for human PNMT was carried out using mouse-human somatic cell hybrids. The PNMT gene was assigned to chromosome 17.
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