| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 263, Issue 17, 8003-8010, Jun, 1988
MC Maiden, MC Jones-Mortimer and PJ Henderson
Department of Biochemistry, University of Cambridge, United Kingdom.
A lambda placMu1 insertion was made into araE, the gene for arabinose- proton symport in Escherichia coli. A phage containing an araE'-'lacZ fusion was recovered from the lysogen and its restriction map compared with that of the 61-min region of the E. coli genome to establish the gene order thyA araE orf lysR lysA galR; araE was transcribed toward orf. A 4.8-kilobase SalI-EcoRI DNA fragment containing araE was subcloned from the phage lambda d(lysA+ galR+ araE+) into the plasmid vector pBR322. From this plasmid a 2.8-kilobase HincII-PvuII DNA fragment including araE was sequenced and also subcloned into the expression vector pAD284. The araE gene was 1416-base pairs long, encoding a hydrophobic protein of 472 amino acids with a calculated Mr of 51,683. The amino acid sequence was homologous with the xylose- proton symporter of E. coli and the glucose transporters from a human hepatoma HepG2 cell line, human erythrocytes, and rat brain. The overexpressed araE gene product was identified in Coomassie-stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels of cell membranes as a protein of apparent Mr 35,000 +/- 1,150. Arabinose protected this protein against reaction with N-ethylmaleimide.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  W.-J. Liang, K. J. Wilson, H. Xie, J. Knol, S. Suzuki, N. G. Rutherford, P. J. F. Henderson, and R. A. Jefferson The gusBC Genes of Escherichia coli Encode a Glucuronide Transport System J. Bacteriol., April 1, 2005; 187(7): 2377 - 2385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Khlebnikov, O. Risa, T. Skaug, T. A. Carrier, and J. D. Keasling Regulatable Arabinose-Inducible Gene Expression System with Consistent Control in All Cells of a Culture J. Bacteriol., December 15, 2000; 182(24): 7029 - 7034. [Abstract] [Full Text]
|
|
|
|
|
|
S. Bost, F. Silva, and D. Belin Transcriptional Activation of ydeA, Which Encodes a Member of the Major Facilitator Superfamily, Interferes with Arabinose Accumulation and Induction of the Escherichia coli Arabinose PBAD Promoter J. Bacteriol., April 1, 1999; 181(7): 2185 - 2191. [Abstract] [Full Text]
|
|
|
|
|
|
W. Ogawa, Y.-M. Kim, T. Mizushima, and T. Tsuchiya Cloning and Expression of the Gene for the Na+-Coupled Serine Transporter from Escherichia coli and Characteristics of the Transporter J. Bacteriol., December 15, 1998; 180(24): 6749 - 6752. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. K. B. Berlyn Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map Microbiol. Mol. Biol. Rev., September 1, 1998; 62(3): 814 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Pao, I. T. Paulsen, and M. H. Saier Jr. Major Facilitator Superfamily Microbiol. Mol. Biol. Rev., March 1, 1998; 62(1): 1 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. P. McDonald, A. R. Walmsley, and P. J. F. Henderson Asparagine 394 in Putative Helix 11 of the Galactose-H+ Symport Protein (GalP) from Escherichia coli Is Associated with the Internal Binding Site for Cytochalasin B and Sugar J. Biol. Chem., June 13, 1997; 272(24): 15189 - 15199. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
