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J. Biol. Chem., Vol. 263, Issue 18, 8724-8726, 06, 1988

A monoclonal antibody that recognizes a cluster of a disaccharide, NeuAc alpha(2----6)GalNAc, in mucin-type glycoproteins

A Kurosaka, H Kitagawa, S Fukui, Y Numata, H Nakada, I Funakoshi, T Kawasaki, T Ogawa, H Iijima and I Yamashina
Division of Biological Engineering, Kyoto Sangyo University, Japan.

The structure of an epitopic carbohydrate recognized by a monoclonal antibody, MLS 102, was determined. A disaccharide, NeuAc alpha (2---- 6)GalNAc, the major prosthetic group of ovine submaxillary mucin (OSM) and related synthetic glycosides, NeuAc alpha(2----6)GalNAc alpha---- Ser, NeuAc alpha(2----6)GalNAc beta----Ser, and NeuAc alpha (2---- 6)GalNAc beta----propyl, reacted with MLS 102 to similar extents, but the reaction was considerably weaker compared to that of OSM. This difference in reactivity could be ascribed to the occurrence of a cluster of the disaccharide on OSM. Purification of MLS 102-reactive antigens from a Triton X-100 extract of LS 180 cells by means of immunoaffinity chromatography gave mucin fractions (cMLS 102 antigen) with an OSM-like domain. Correlation between the content of the disaccharide, NeuAc alpha(2----6)GalNAc, in mucins and their reactivity with MLS 102 was observed.
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