JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by LaPointe, M. C.
Right arrow Articles by Gardner, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by LaPointe, M. C.
Right arrow Articles by Gardner, D. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J. Biol. Chem., Vol. 263, Issue 19, 9075-9078, 07, 1988

Upstream sequences confer atrial-specific expression on the human atrial natriuretic factor gene

MC LaPointe, JP Wu, B Greenberg and DG Gardner
Department of Medicine, University of California, San Francisco 94143.

5'-Flanking sequences from the human atrial natriuretic factor (hANF) gene were subcloned into a reporter plasmid (pSVOCAT) and transfected into primary cultures of neonatal rat atrial cardiocytes. Hybrid hANFCAT genes containing either 2500 or 409 base pairs of 5'-flanking sequence DNA were expressed at similar levels. When sequences between - 409 and -332 were deleted, reporter gene (CAT) activity decreased significantly. Expression of the hANFCAT constructs was specific for atrial cells, as no expression was detected in primary cultures of ventricular cardiocytes or nonmyocardial cells derived from the neonatal hearts. Correct transcription start sites for the transfected hANF genes were confirmed by S1 nuclease mapping and RNase protection analysis. A "gel shift" assay was used to identify a specific cardiac nuclear protein which bound to the 5'-flanking sequence of the hANF gene. A 192-base pair PvuII fragment (-400 to -208) associated with a protein in these extracts in a tissue- and sequence-specific fashion. These findings indicate that the DNA sequence between -409 and -332 in the hANF gene harbors a tissue-specific element whose activity may involve association with a cardiac-specific nuclear protein.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J Am Coll CardiolHome page
S. Rubattu, G. Bigatti, A. Evangelista, C. Lanzani, R. Stanzione, L. Zagato, P. Manunta, S. Marchitti, V. Venturelli, G. Bianchi, et al.
Association of Atrial Natriuretic Peptide and Type A Natriuretic Peptide Receptor Gene Polymorphisms With Left Ventricular Mass in Human Essential Hypertension
J. Am. Coll. Cardiol., August 1, 2006; 48(3): 499 - 505.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
A. C. Houweling, M. M. van Borren, A. F.M. Moorman, and V. M. Christoffels
Expression and regulation of the atrial natriuretic factor encoding gene Nppa during development and disease
Cardiovasc Res, September 1, 2005; 67(4): 583 - 593.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
B. Kovacic-Milivojevic, F. Roediger, E. A.C. Almeida, C. H. Damsky, D. G. Gardner, and D. Ilic
Focal Adhesion Kinase and p130Cas Mediate Both Sarcomeric Organization and Activation of Genes Associated with Cardiac Myocyte Hypertrophy
Mol. Biol. Cell, August 1, 2001; 12(8): 2290 - 2307.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Kovacic, C. H. Damsky, and D. G. Gardner
c-Src Activation Plays a Role in Endothelin-dependent Hypertrophy of the Cardiac Myocyte
J. Biol. Chem., December 25, 1998; 273(52): 35185 - 35193.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. C.J. Ribeiro, R. R. Cavalieri, N. Lomri, C. M. Rahmaoui, J. D. Baxter, and B. F. Scharschmidt
Thyroid Hormone Export Regulates Cellular Hormone Content and Response
J. Biol. Chem., July 19, 1996; 271(29): 17147 - 17151.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1988 by the American Society for Biochemistry and Molecular Biology.