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J. Biol. Chem., Vol. 263, Issue 19, 9087-9093, 07, 1988

Amino acid sequence of bovine spleen cathepsin B

B Meloun, M Baudys, J Pohl, M Pavlik and V Kostka
Department of Biochemistry, Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Science, Prague.

The complete amino acid sequence of bovine spleen cathepsin B was determined by manual and automatic Edman degradation of fragments prepared by proteolytic or chemical digestion of the enzyme. The single- chain form of the enzyme consists of 253 amino acid residues and its Mr is 27,468 (carbohydrate moiety not included). The light chain (residues 1-47) and the heavy chain (residues 50-253) of the enzyme are linked by the sequence -Gly-Arg (residues 48 and 49) in the single-chain form. Bovine spleen cathepsin B shows 80% sequence homology with cathepsins B from other species. An outstanding feature of bovine spleen cathepsin B not observed with the other cathepsins B is the presence of two additional half-cystine residues.
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