|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 263, Issue 2, 740-744, 01, 1988
PB Iynedjian, A Gjinovci and AE Renold
The purpose of this work was to investigate the molecular mechanism
responsible for the induction of hepatic glucokinase in diabetic rats
acutely treated with insulin. Experimental diabetes was provoked by
injection of streptozotocin 8-10 days before the experiments. Regular
insulin was given by three intraperitoneal injections at 8-h intervals, and
the time course of glucokinase induction was followed over a time period of
24 h. The amount of glucokinase in liver was estimated by Western blotting
of total cytosol protein with affinity-purified antibodies, as well as by
conventional enzyme activity assay. Both measurements showed that
glucokinase was reduced by more than 90% in the livers of diabetic rats as
compared to normal controls. Following insulin administration, the amount
(and activity) of glucokinase increased in a time-dependent fashion, after
an initial lag of 4 h, to reach 65% of the nondiabetic control level 24 h
after the initial dose of insulin. Northern blot analysis with a cloned
cDNA probe was used to quantitate glucokinase mRNA. In contrast with the
slow onset of enzyme accumulation, the amount of glucokinase mRNA was shown
to be increased dramatically as early as 1 h after insulin administration.
The abundance of specific mRNA increased until 8 h after the initial dose
of insulin. Subsequently, the level of the mRNA decayed rapidly so that
little message was left after 16 h and virtually none after 24 h. Run- on
transcription experiments with isolated nuclei showed that the rate of
transcription of the glucokinase gene was increased about 20-fold within 45
min of insulin administration and returned to the prestimulation level
after 8 h. From these data, it was concluded that the induction of
glucokinase resulted primarily from a burst in the transcriptional activity
of the gene, leading to a short-term accumulation of glucokinase mRNA. The
more sustained elevation of the enzyme level can be accounted for by the
long half-life of the enzyme (greater than 30 h). The virtually immediate
activation of glucokinase gene transcription suggests a direct effect of
insulin on the liver cell.
Stimulation by insulin of glucokinase gene transcription in liver of diabetic rats
Institut de Biochimie Clinique, University of Geneva School of Medicine, Switzerland.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Hirota, J.-i. Sakamaki, J. Ishida, Y. Shimamoto, S. Nishihara, N. Kodama, K. Ohta, M. Yamamoto, K. Tanimoto, and A. Fukamizu A Combination of HNF-4 and Foxo1 Is Required for Reciprocal Transcriptional Regulation of Glucokinase and Glucose-6-phosphatase Genes in Response to Fasting and Feeding J. Biol. Chem., November 21, 2008; 283(47): 32432 - 32441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Pino, K.-A. Kim, K. D. Shelton, J. Lindner, S. Odili, C. Li, H. W. Collins, M. Shiota, F. M. Matschinsky, and M. A. Magnuson Glucokinase Thermolability and Hepatic Regulatory Protein Binding Are Essential Factors for Predicting the Blood Glucose Phenotype of Missense Mutations J. Biol. Chem., May 4, 2007; 282(18): 13906 - 13916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. J. Jung, M.-K. Lee, Y. B. Park, S.-M. Jeon, and M.-S. Choi Antihyperglycemic and Antioxidant Properties of Caffeic Acid in db/db Mice J. Pharmacol. Exp. Ther., August 1, 2006; 318(2): 476 - 483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Baltrusch, F. Francini, S. Lenzen, and M. Tiedge Interaction of Glucokinase With the Liver Regulatory Protein Is Conferred by Leucine-Asparagine Motifs of the Enzyme Diabetes, October 1, 2005; 54(10): 2829 - 2837. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Yeo, R. L. P. G. Jentjens, G. A. Wallis, and A. E. Jeukendrup Caffeine increases exogenous carbohydrate oxidation during exercise J Appl Physiol, September 1, 2005; 99(3): 844 - 850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. J. Jung, M.-K. Lee, K.-S. Jeong, and M.-S. Choi The Hypoglycemic Effects of Hesperidin and Naringin Are Partly Mediated by Hepatic Glucose-Regulating Enzymes in C57BL/KsJ-db/db Mice J. Nutr., October 1, 2004; 134(10): 2499 - 2503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Basu, A. Basu, C. M. Johnson, W. F. Schwenk, and R. A. Rizza Insulin Dose-Response Curves for Stimulation of Splanchnic Glucose Uptake and Suppression of Endogenous Glucose Production Differ in Nondiabetic Humans and Are Abnormal in People With Type 2 Diabetes Diabetes, August 1, 2004; 53(8): 2042 - 2050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-Y. Kim, H.-i. Kim, T.-H. Kim, S.-S. Im, S.-K. Park, I.-K. Lee, K.-S. Kim, and Y.-H. Ahn SREBP-1c Mediates the Insulin-dependent Hepatic Glucokinase Expression J. Biol. Chem., July 16, 2004; 279(29): 30823 - 30829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Collier and D. K. Scott Sweet Changes: Glucose Homeostasis Can Be Altered by Manipulating Genes Controlling Hepatic Glucose Metabolism Mol. Endocrinol., May 1, 2004; 18(5): 1051 - 1063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Wu, D. A. Okar, A. K. Stoeckman, L.-J. Peng, A. H. Herrera, J. E. Herrera, H. C. Towle, and A. J. Lange A Potential Role for Fructose-2,6-Bisphosphate in the Stimulation of Hepatic Glucokinase Gene Expression Endocrinology, February 1, 2004; 145(2): 650 - 658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Murphy, G. Regan, I. G. Bogdarina, A. J.L. Clark, R. A. Iles, R. D. Cohen, G. A. Hitman, C. L. Berry, Z. Coade, C. J. Petry, et al. Fetal Programming of Perivenous Glucose Uptake Reveals a Regulatory Mechanism Governing Hepatic Glucose Output During Refeeding Diabetes, June 1, 2003; 52(6): 1326 - 1332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Ribaux, A. Gjinovci, H. B. Sadowski, and P. B. Iynedjian Discrimination between Signaling Pathways in Regulation of Specific Gene Expression by Insulin and Growth Hormone in Hepatocytes Endocrinology, October 1, 2002; 143(10): 3766 - 3772. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Wu, D. A. Okar, C. B. Newgard, and A. J. Lange Increasing fructose 2,6-bisphosphate overcomes hepatic insulin resistance of type 2 diabetes Am J Physiol Endocrinol Metab, January 1, 2002; 282(1): E38 - E45. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Becard, I. Hainault, D. Azzout-Marniche, L. Bertry-Coussot, P. Ferre, and F. Foufelle Adenovirus-Mediated Overexpression of Sterol Regulatory Element Binding Protein-1c Mimics Insulin Effects on Hepatic Gene Expression and Glucose Homeostasis in Diabetic Mice Diabetes, November 1, 2001; 50(11): 2425 - 2430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shiota, C. Postic, Y. Fujimoto, T. L. Jetton, K. Dixon, D. Pan, J. Grimsby, J. F. Grippo, M. A. Magnuson, and A. D. Cherrington Glucokinase Gene Locus Transgenic Mice Are Resistant to the Development of Obesity-Induced Type 2 Diabetes Diabetes, March 1, 2001; 50(3): 622 - 629. [Abstract] [Full Text]
|
|
|
|
|
|
D. Sawka-Verhelle, S. Tartare-Deckert, J.-F. Decaux, J. Girard, and E. Van Obberghen Stat 5B, Activated by Insulin in a Jak-Independent Fashion, Plays a Role in Glucokinase Gene Transcription Endocrinology, June 1, 2000; 141(6): 1977 - 1988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Shiota, J. Coffey, J. Grimsby, J. F. Grippo, and M. A. Magnuson Nuclear Import of Hepatic Glucokinase Depends upon Glucokinase Regulatory Protein, whereas Export Is Due to a Nuclear Export Signal Sequence in Glucokinase J. Biol. Chem., December 24, 1999; 274(52): 37125 - 37130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Farrelly, K. S. Brown, A. Tieman, J. Ren, S. A. Lira, D. Hagan, R. Gregg, K. A. Mookhtiar, and N. Hariharan Mice mutant for glucokinase regulatory protein exhibit decreased liver glucokinase: A sequestration mechanism in metabolic regulation PNAS, December 7, 1999; 96(25): 14511 - 14516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Foretz, C. Guichard, P. Ferre, and F. Foufelle Sterol regulatory element binding protein-1c is a major mediator of insulin action on the hepatic expression of glucokinase and lipogenesis-related genes PNAS, October 26, 1999; 96(22): 12737 - 12742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Wagle, S. Jivraj, G. L. Garlock, and S. R. Stapleton Insulin Regulation of Glucose-6-phosphate Dehydrogenase Gene Expression Is Rapamycin-sensitive and Requires Phosphatidylinositol 3-Kinase J. Biol. Chem., June 12, 1998; 273(24): 14968 - 14974. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Koopmans, L. Mandarino, and R. A. Defronzo Time course of insulin action on tissue-specific intracellular glucose metabolism in normal rats Am J Physiol Endocrinol Metab, April 1, 1998; 274(4): E642 - E650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Massillon, W. Chen, N. Barzilai, D. Prus-Wertheimer, M. Hawkins, R. Liu, R. Taub, and L. Rossetti Carbon Flux via the Pentose Phosphate Pathway Regulates the Hepatic Expression of the Glucose-6-phosphatase and Phosphoenolpyruvate Carboxykinase Genes in Conscious Rats J. Biol. Chem., January 2, 1998; 273(1): 228 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. D. Niswender, C. Postic, T. L. Jetton, B. D. Bennett, D. W. Piston, S. Efrat, and M. A. Magnuson Cell-specific Expression and Regulation of a Glucokinase Gene Locus Transgene J. Biol. Chem., September 5, 1997; 272(36): 22564 - 22569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mitanchez, B. Doiron, R. Chen, and A. Kahn Glucose-Stimulated Genes and Prospects of Gene Therapy for Type I Diabetes Endocr. Rev., August 1, 1997; 18(4): 520 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. B. Iynedjian, S. Marie, H. Wang, A. Gjinovci, and K. Nazaryan Liver-specific Enhancer of the Glucokinase Gene J. Biol. Chem., November 15, 1996; 271(46): 29113 - 29120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. V. Rao, S. Pugazhenthi, and R. L. Khandelwal The Effects of Streptozotocin-induced Diabetes and Insulin Supplementation on Expression of the Glycogen Phosphorylase Gene in Rat Liver J. Biol. Chem., October 20, 1995; 270(42): 24955 - 24960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. O'Brien, P. Lucas, C. Forest, M. Magnuson, and D. Granner Identification of a sequence in the PEPCK gene that mediates a negative effect of insulin on transcription Science, August 3, 1990; 249(4968): 533 - 537. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |