| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 263, Issue 20, 9598-9606, 07, 1988
ES Furfine and RH Abeles
Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02254.
Extracts of Klebsiella pneumoniae oxidatively convert 1-phospho-5-S- methylthioribose (1-PMTR) to alpha-keto-gamma-methylthiobutyrate, a precursor of methionine, and to S-methylthiopropionate and formate. One equivalent of formate is produced per equivalent of alpha-keto-gamma- methylthiobutyrate and two equivalents of formate per equivalent of methylthiopropionate. Two compounds were identified as intermediates in this reaction sequence: 1-phospho-5-S-methylthioribulose (1-PMT- ribulose) and 1-phospho-2,3-diketo-5-S-methylpentane. The enzyme, 1- PMTR isomerase, which converts 1-PMTR to 1-PMT-ribulose was highly purified. In addition, a protein fraction was isolated which converts 1- PMT-ribulose to the phosphodiketone. A second protein fraction was isolated that converts the phosphodiketone to an intermediate which has not been isolated so far. This intermediate is oxidatively converted to alpha-keto-gamma-methylthiobutyrate and S-methylthiopropionate by a third protein fraction. Methylthiopropionate is not derived from free alpha-keto-gamma-methylthiobutyrate.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Ashida, Y. Saito, T. Nakano, N. Tandeau de Marsac, A. Sekowska, A. Danchin, and A. Yokota RuBisCO-like proteins as the enolase enzyme in the methionine salvage pathway: functional and evolutionary relationships between RuBisCO-like proteins and photosynthetic RuBisCO J. Exp. Bot., May 1, 2008; 59(7): 1543 - 1554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bumann, S. Djafarzadeh, A. E. Oberholzer, P. Bigler, M. Altmann, H. Trachsel, and U. Baumann Crystal Structure of Yeast Ypr118w, a Methylthioribose-1-phosphate Isomerase Related to Regulatory eIF2B Subunits J. Biol. Chem., August 27, 2004; 279(35): 37087 - 37094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Subhi, P. Diegelman, C. W. Porter, B. Tang, Z. J. Lu, G. D. Markham, and W. D. Kruger Methylthioadenosine Phosphorylase Regulates Ornithine Decarboxylase by Production of Downstream Metabolites J. Biol. Chem., December 12, 2003; 278(50): 49868 - 49873. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ashida, Y. Saito, C. Kojima, K. Kobayashi, N. Ogasawara, and A. Yokota A Functional Link Between RuBisCO-like Protein of Bacillus and Photosynthetic RuBisCO Science, October 10, 2003; 302(5643): 286 - 290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Christopher, P. Diegelman, C. W. Porter, and W. D. Kruger Methylthioadenosine Phosphorylase, a Gene Frequently Codeleted with p16cdkN2a/ARF, Acts as a Tumor Suppressor in a Breast Cancer Cell Line Cancer Res., November 15, 2002; 62(22): 6639 - 6644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Murphy, F. J. Grundy, and T. M. Henkin Prediction of Gene Function in Methylthioadenosine Recycling from Regulatory Signals J. Bacteriol., April 15, 2002; 184(8): 2314 - 2318. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Dai, P. C. Wensink, and R. H. Abeles One Protein, Two Enzymes J. Biol. Chem., January 15, 1999; 274(3): 1193 - 1195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Wray and R. H. Abeles The Methionine Salvage Pathway in Klebsiella pneumoniae and Rat Liver J. Biol. Chem., February 17, 1995; 270(7): 3147 - 3153. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
