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J. Biol. Chem., Vol. 263, Issue 20, 9767-9775, 07, 1988
P Boulanger and L Letellier
The hypothesis of a channel-mediated transport of phage DNA into
Escherichia coli cytoplasmic membrane has been formulated for a long time.
In this paper, we present experimental evidence in favor of this proposal.
We have analyzed the kinetics of the K+ efflux induced by T4 phage and
ghosts (phage depleted of DNA) using a potassium selective electrode. We
show that the K+ efflux is not catalyzed by the K+ transport systems. The
Km of K+ efflux is the same for phage and ghosts. The rate of K+ efflux is
linearly related to the multiplicity of infection. This suggests that phage
and ghosts induce the formation of similar channels and that one channel is
induced by one virion. The K+ efflux is associated with an influx of H+ and
Na+ or Li+ which compete for entry through the channel. These ion fluxes
may be responsible for the cell depolarization. The phage-induced channels
allow the passage of DNA. They are only transiently opened, and their
closing leads to cellular repolarization. The ghost-induced channels remain
open. The insertion and conformation of the channels in the membrane depend
on the temperature and their confirmation is voltage- dependent. We give an
estimate of their size.
Characterization of ion channels involved in the penetration of phage T4 DNA into Escherichia coli cells
Laboratoire des Biomembranes, U.A. 1116, Universite Paris Sud, Orsay, France.
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