| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 263, Issue 25, 12175-12178, Sep, 1988
A Persechini and RH Kretsinger
Using site-directed mutagenesis we have created an altered calmodulin in
which Gln-3 and Thr-146 have both been replaced by cysteines. We have
reacted this protein with the bifunctional reagent, bismaleimidohexane,
forming an intramolecular cross-link between the two cysteines. In the
crystal structure of native calmodulin alpha- carbons at positions 3 and
146 are 37 A apart. In the bismaleimidohexane cross-linked protein these
atoms can be no more than 19 A apart, and model building studies indicate
that there is probably a bend in the central helix of calmodulin. A second
modified calmodulin was generated by cleaving the central helix of the
cross-linked protein at Lys-77 with trypsin. In this molecule, the two
lobes of calmodulin are joined solely by the bismaleimidohexane cross-link,
which bridges Cys-3 and Cys-146. Vm and Kact values for activation of
myosin light chain kinase activity by the cross-linked and
cross-linked/trypsinized proteins are not significantly different from
those for the control protein. This result indicates that one role for the
central helix may be to serve as a flexible tether between the calmodulin
lobes. This is consistent with a model calmodulin-enzyme complex in which
the central helix is bent, and the two lobes exert a concerted effect. A
detailed model of this type has been proposed for the calmodulin-myosin
light chain kinase complex (Persechini, A. and Kretsinger, R.H. (1988) J.
Cardiovasc. Pharmacol., in press).
The central helix of calmodulin functions as a flexible tether
Department of Biology, University of Virginia, Charlottesville 22901.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. E. Simpson, A. Ciruela, and D. M. F. Cooper The Role of Calmodulin Recruitment in Ca2+ Stimulation of Adenylyl Cyclase Type 8 J. Biol. Chem., June 23, 2006; 281(25): 17379 - 17389. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ikura and J. B. Ames Genetic polymorphism and protein conformational plasticity in the calmodulin superfamily: Two ways to promote multifunctionality PNAS, January 31, 2006; 103(5): 1159 - 1164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Alexandrov, U. Lehnert, N. Echols, D. Milburn, D. Engelman, and M. Gerstein Normal modes for predicting protein motions: A comprehensive database assessment and associated Web tool Protein Sci., March 1, 2005; 14(3): 633 - 643. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Andre, T. Kesvatera, B. Jonsson, K. S. Akerfeldt, and S. Linse The Role of Electrostatic Interactions in Calmodulin-Peptide Complex Formation Biophys. J., September 1, 2004; 87(3): 1929 - 1938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Shepherd and H. J. Vogel A Molecular Dynamics Study of Ca2+-Calmodulin: Evidence of Interdomain Coupling and Structural Collapse on the Nanosecond Timescale Biophys. J., August 1, 2004; 87(2): 780 - 791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nousiainen, P. J. Derrick, D. Lafitte, and P. Vainiotalo Relative Affinity Constants by Electrospray Ionization and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: Calmodulin Binding to Peptide Analogs of Myosin Light Chain Kinase Biophys. J., July 1, 2003; 85(1): 491 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Veeraraghavan, P. A. Fagan, H. Hu, V. Lee, J. F. Harper, B. Huang, and W. J. Chazin Structural Independence of the Two EF-hand Domains of Caltractin J. Biol. Chem., August 2, 2002; 277(32): 28564 - 28571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Myre and D. H. O'Day Nucleomorphin. A NOVEL, ACIDIC, NUCLEAR CALMODULIN-BINDING PROTEIN FROM DICTYOSTELIUM THAT REGULATES NUCLEAR NUMBER J. Biol. Chem., May 24, 2002; 277(22): 19735 - 19744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hayashi, M. Matsubara, Y. Jinbo, K. Titani, Y. Izumi, and N. Matsushima Nef of HIV-1 interacts directly with calcium-bound calmodulin Protein Sci., March 1, 2002; 11(3): 529 - 537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sun and T. C. Squier Ordered and Cooperative Binding of Opposing Globular Domains of Calmodulin to the Plasma Membrane Ca-ATPase J. Biol. Chem., January 21, 2000; 275(3): 1731 - 1738. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Jurado, P. S. Chockalingam, and H. W. Jarrett Apocalmodulin Physiol Rev, July 1, 1999; 79(3): 661 - 682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Persechini, P. M. Stemmer, and I. Ohashi Localization of Unique Functional Determinants in the Calmodulin Lobes to Individual EF Hands J. Biol. Chem., December 13, 1996; 271(50): 32217 - 32225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Persechini, K. J. Gansz, and R. J. Paresi A Role in Enzyme Activation for the N-terminal Leader Sequence in Calmodulin J. Biol. Chem., August 9, 1996; 271(32): 19279 - 19282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Johnson, C. Snyder, M. Walsh, and M. Flynn Effects of Myosin Light Chain Kinase and Peptides on Ca[IMAGE] Exchange with the N- and C-terminal Ca[IMAGE] Binding Sites of Calmodulin J. Biol. Chem., January 12, 1996; 271(2): 761 - 767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ho, T.-L. L. Chen, and R. L. Chisholm Both the Amino and Carboxyl Termini of Dictyostelium Myosin Essential Light Chain Are Required for Binding to Myosin Heavy Chain J. Biol. Chem., November 17, 1995; 270(46): 27977 - 27981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Craescu, A. Bouhss, J.ël Mispelter, E. Diesis, A. Popescu, M. Chiriac, and O. Bârzu Calmodulin Binding of a Peptide Derived from the Regulatory Domain of Bordetella pertussis Adenylate Cyclase J. Biol. Chem., March 31, 1995; 270(13): 7088 - 7096. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Kretsinger Calmodulin and myosin light chain kinase: how helices are bent Science, October 2, 1992; 258(5079): 50 - 51. [PDF]
|
|
|
|
|
|
W. Meador, A. Means, and F. Quiocho Target enzyme recognition by calmodulin: 2.4 A structure of a calmodulin-peptide complex Science, August 28, 1992; 257(5074): 1251 - 1255. [Abstract] [PDF]
|
|
|
|
|
|
M Ikura, G. Clore, A. Gronenborn, G Zhu, C. Klee, and A Bax Solution structure of a calmodulin-target peptide complex by multidimensional NMR Science, May 1, 1992; 256(5057): 632 - 638. [Abstract] [PDF]
|
|
|
|
|
|
J. K. Krueger, S. C. Gallagher, G. Zhi, R. Geguchadze, A. Persechini, J. T. Stull, and J. Trewhella Activation of Myosin Light Chain Kinase Requires Translocation of Bound Calmodulin J. Biol. Chem., February 9, 2001; 276(7): 4535 - 4538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Cobb and D. M. Roberts Structural Requirements for N-Trimethylation of Lysine 115 of Calmodulin J. Biol. Chem., June 16, 2000; 275(25): 18969 - 18975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Jenkins, M. J. Wolf, D. J. Mancuso, and R. W. Gross Identification of the Calmodulin-binding Domain of Recombinant Calcium-independent Phospholipase A2beta . IMPLICATIONS FOR STRUCTURE AND FUNCTION J. Biol. Chem., March 2, 2001; 276(10): 7129 - 7135. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
