J. Biol. Chem., Vol. 263, Issue 25, 12373-12377, 09, 1988
Mass spectrometric determination of the inorganic carbon species assimilated by photoautotrophic cells of Euphorbia characias L
F Rebeille, P Gans, P Chagvardieff, M Pean, P Tapie and P Thibault
Department de Biologie, C.E.N. de Cadarache, Saint-Paul-lez-Durance, France.
The chemical forms of inorganic carbon, CO2 or HCO3-, incorporated during
photosynthesis in photoautotrophic Euphorbia characias cell suspension
cultures were determined in experiments using 13CO2 and a mass spectrometry
technique. From the equations of the CO2 hydration reaction, a kinetic
model was first developed, and the effect of photosynthesis on the external
CO2 concentration was simulated. It was predicted from this model that CO2
and HCO3- uptakes could be differentiated by recording only the CO2
variation rate in the external medium, successively in absence then in
presence of an exogenous carbonic anhydrase activity. The results obtained
with either CO2-grown or air-grown photoautotrophic cells were in good
agreement with the model and demonstrated that CO2 was the sole species
taken up during photosynthesis. In addition no accumulation of inorganic
carbon within the cells was observed in the light. Similarly, in dark, CO2
was the only species released by respiration in the external medium.
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