HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Luetje, C. W. Articles by Nathanson, N. M. Search for Related Content PubMed PubMed Citation Articles by Luetje, C. W. Articles by Nathanson, N. M. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 263, Issue 26, 13357-13365, Sep, 1988

Differential tissue expression and developmental regulation of guanine nucleotide binding regulatory proteins and their messenger RNAs in rat heart

CW Luetje, KM Tietje, JL Christian and NM Nathanson
Department of Pharmacology, University of Washington School of Medicine, Seattle 98195.

The expression and developmental regulation of the alpha and beta subunits of the guanine nucleotide binding regulatory proteins, Gi and Go, were examined in rat atria and ventricles. Protein levels were determined by quantitative immunoblot analysis using affinity purified monospecific antibodies. Northern blot and dot blot analyses were used to characterize and quantitate relative amounts of mRNA encoding these G protein subunits. The concentrations of Go alpha, Gi alpha, and beta subunit protein were found to be greater in adult atrial than in adult ventricular membranes (5.2-, 1.5-, and 2.8-fold, respectively). A corresponding 3.4-fold difference in Go alpha mRNA level was also observed, as well as a 1.3-fold difference in Gi alpha-3 mRNA level. No difference was seen between the amount of beta, Gi alpha-1, Gi alpha-2 mRNA in adult atria and adult ventricles. Comparison of neonatal and adult tissues revealed a developmental decrease in ventricular Gi alpha protein and Gi alpha-2 mRNA levels (70 and 47%, respectively). Developmental decreases were also observed in the amount of mRNA encoding beta and Go alpha in ventricles (47 and 61%, respectively), and beta and Gi alpha-2 in atria (40 and 36%, respectively), while a developmental increase in atrial Gi alpha-3 mRNA levels was observed (57%). These results demonstrate differences in the expression of G protein subunits in rat atria and ventricles, as well as regulation of the levels of these subunits during cardiac development.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Mizuno, A. Kamiya, T. Kawada, T. Miyamoto, S. Shimizu, and M. Sugimachi Muscarinic potassium channels augment dynamic and static heart rate responses to vagal stimulation Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1564 - H1570. [Abstract] [Full Text] [PDF]

				J.-C. Peter, J. Tugler, P. Eftekhari, D. Maurice, J. Hoebeke, and J.-C. Roegel Effects on heart rate of an anti-M2 acetylcholine receptor immune response in mice FASEB J, June 1, 2005; 19(8): 943 - 949. [Abstract] [Full Text] [PDF]

				T. Ivanina, D. Varon, S. Peleg, I. Rishal, Y. Porozov, C. W. Dessauer, T. Keren-Raifman, and N. Dascal G{alpha}i1 and G{alpha}i3 Differentially Interact with, and Regulate, the G Protein-activated K+ Channel J. Biol. Chem., April 23, 2004; 279(17): 17260 - 17268. [Abstract] [Full Text] [PDF]

				V. O. Rybin, E. Pak, S. Alcott, and S. F. Steinberg Developmental Changes in {beta}2-Adrenergic Receptor Signaling in Ventricular Myocytes: the Role of Gi proteins and Caveolae Microdomains Mol. Pharmacol., June 1, 2003; 63(6): 1338 - 1348. [Abstract] [Full Text] [PDF]

				M. Yamada The Role of Muscarinic K+ Channels in the Negative Chronotropic Effect of a Muscarinic Agonist J. Pharmacol. Exp. Ther., February 1, 2002; 300(2): 681 - 687. [Abstract] [Full Text] [PDF]

				N. Dzimiri Regulation of beta -Adrenoceptor Signaling in Cardiac Function and Disease Pharmacol. Rev., September 1, 1999; 51(3): 465 - 502. [Abstract] [Full Text] [PDF]

				M. O. Sowell, C. Ye, D. A. Ricupero, S. Hansen, S. J. Quinn, P. M. Vassilev, and R. M. Mortensen Targeted inactivation of alpha i2 or alpha i3 disrupts activation of the cardiac muscarinic K+ channel, IK+Ach, in intact cells PNAS, July 22, 1997; 94(15): 7921 - 7926. [Abstract] [Full Text] [PDF]

				S. F. Steinberg, H. Zhang, E. Pak, G. Pagnotta, and P. A. Boyden Characteristics of the ß-Adrenergic Receptor Complex in the Epicardial Border Zone of the 5-Day Infarcted Canine Heart Circulation, June 1, 1995; 91(11): 2824 - 2833. [Abstract] [Full Text]