| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 263, Issue 28, 14002-14007, 10, 1988
BJ Bowman, R Allen, MA Wechser and EJ Bowman
Department of Biology, Thimann Laboratories, University of California, Santa Cruz 95064.
In partially purified preparations of the vacuolar ATPase from Neurospora crassa, the two most prominent components are polypeptides of Mr = 70,000 and 60,000. We previously reported the isolation of the gene vma-1, which encodes the Mr = 70,000 polypeptide, and presented evidence that the polypeptide contains the site of ATP hydrolysis (Bowman, E. J., Tenney, K., and Bowman, B. J. (1988) J. Biol. Chem. 263, 13994-14001). We now report the isolation of a gene (designated vma-2), that encodes the Mr = 60,000 polypeptide. Analysis of the DNA sequence shows that the polypeptide has 513 amino acids and a molecular mass of 56,808 daltons (and will thus be referred to as the 57-kDa polypeptide). It is fairly rich in polar amino acids and has no apparent membrane-spanning domains. The vma-2 gene contains five short introns (55-71 bases), all clustered in the 5' end of the coding region. The gene maps to the right arm of linkage group II, near 5 S RNA gene 3. Thus, it is unlinked to vma-1 and to other known ATPase genes in N. crassa. The 57-kDa polypeptide shows 25% amino acid sequence identity with the vma-1 gene product. It shows essentially the same degree of similarity (25-28%) to both the alpha and beta subunits of F0F1 ATPases. Analysis of specific regions of the 57-kDa polypeptide, however, suggests it may have a function like that of the alpha subunit in F0F1 ATPases. The data indicate that all four types of ATPase polypeptides have evolved from a common ancestor and that the vacuolar-type ATPases have a structure surprisingly similar to that of the F0F1 ATPases.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. G. Paunescu, L. M. Russo, N. Da Silva, J. Kovacikova, N. Mohebbi, A. N. Van Hoek, M. McKee, C. A. Wagner, S. Breton, and D. Brown Compensatory membrane expression of the V-ATPase B2 subunit isoform in renal medullary intercalated cells of B1-deficient mice Am J Physiol Renal Physiol, December 1, 2007; 293(6): F1915 - F1926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Chavez, E. J. Bowman, J. C. Reidling, K. H. Haw, and B. J. Bowman Analysis of Strains with Mutations in Six Genes Encoding Subunits of the V-ATPase: EUKARYOTES DIFFER IN THE COMPOSITION OF THE V0 SECTOR OF THE ENZYME J. Biol. Chem., September 15, 2006; 281(37): 27052 - 27062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Kane The Where, When, and How of Organelle Acidification by the Yeast Vacuolar H+-ATPase Microbiol. Mol. Biol. Rev., March 1, 2006; 70(1): 177 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Inoue and M. Forgac Cysteine-mediated Cross-linking Indicates That Subunit C of the V-ATPase Is in Close Proximity to Subunits E and G of the V1 Domain and Subunit a of the V0 Domain J. Biol. Chem., July 29, 2005; 280(30): 27896 - 27903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Shao and M. Forgac Involvement of the Nonhomologous Region of Subunit A of the Yeast V-ATPase in Coupling and in Vivo Dissociation J. Biol. Chem., November 19, 2004; 279(47): 48663 - 48670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fethiere, D. Venzke, M. Diepholz, A. Seybert, A. Geerlof, M. Gentzel, M. Wilm, and B. Bottcher Building the Stator of the Yeast Vacuolar-ATPase: SPECIFIC INTERACTION BETWEEN SUBUNITS E AND G J. Biol. Chem., September 24, 2004; 279(39): 40670 - 40676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. G. Paunescu, N. D. Silva, V. Marshansky, M. McKee, S. Breton, and D. Brown Expression of the 56-kDa B2 subunit isoform of the vacuolar H+-ATPase in proton-secreting cells of the kidney and epididymis Am J Physiol Cell Physiol, July 1, 2004; 287(1): C149 - C162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Shao, T. Nishi, S. Kawasaki-Nishi, and M. Forgac Mutational Analysis of the Non-homologous Region of Subunit A of the Yeast V-ATPase J. Biol. Chem., April 4, 2003; 278(15): 12985 - 12991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nishi, S. Kawasaki-Nishi, and M. Forgac The First Putative Transmembrane Segment of Subunit c" (Vma16p) of the Yeast V-ATPase Is Not Necessary for Function J. Biol. Chem., February 14, 2003; 278(8): 5821 - 5827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Vasilyeva, Q. Liu, K. J. MacLeod, J. D. Baleja, and M. Forgac Cysteine Scanning Mutagenesis of the Noncatalytic Nucleotide Binding Site of the Yeast V-ATPase J. Biol. Chem., January 7, 2000; 275(1): 255 - 260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Bowman and B. Bowman Cellular role of the V-ATPase in Neurospora crassa: analysis of mutants resistant to concanamycin or lacking the catalytic subunit A J. Exp. Biol., January 1, 2000; 203(1): 97 - 106. [Abstract]
|
|
|
|
 |
|
 N. Nelson and W. R. Harvey Vacuolar and Plasma Membrane Proton-Adenosinetriphosphatases Physiol Rev, April 1, 1999; 79(2): 361 - 385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-H. Leng, M. F. Manolson, and M. Forgac Function of the COOH-terminal Domain of Vph1p in Activity and Assembly of the Yeast V-ATPase J. Biol. Chem., March 20, 1998; 273(12): 6717 - 6723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhou, S.-B. Peng, B. P. Crider, C. Slaughter, X.-S. Xie, and D. K. Stone Molecular Characterization of the 50- and 57-kDa Subunits of the Bovine Vacuolar Proton Pump J. Biol. Chem., March 6, 1998; 273(10): 5878 - 5884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. MacLeod, E. Vasilyeva, J. D. Baleja, and M. Forgac Mutational Analysis of the Nucleotide Binding Sites of the Yeast Vacuolar Proton-translocating ATPase J. Biol. Chem., January 2, 1998; 273(1): 150 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Liu, X.-H. Leng, P. R. Newman, E. Vasilyeva, P. M. Kane, and M. Forgac Site-directed Mutagenesis of the Yeast V-ATPase A Subunit J. Biol. Chem., May 2, 1997; 272(18): 11750 - 11756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-H. Leng, M. F. Manolson, Q. Liu, and M. Forgac Site-directed Mutagenesis of the 100-kDa Subunit (Vph1p) of the Yeast Vacuolar (H+)-ATPase J. Biol. Chem., September 13, 1996; 271(37): 22487 - 22493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Vasilyeva and M. Forgac 3'-O-(4-Benzoyl)benzoyladenosine 5'-Triphosphate Inhibits Activity of the Vacuolar (H+)-ATPase from Bovine Brain Clathrin-coated Vesicles by Modification of a Rapidly Exchangeable, Noncatalytic Nucleotide Binding Site on the B Subunit J. Biol. Chem., May 31, 1996; 271(22): 12775 - 12782. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-B. Peng, B. P. Crider, S. J. Tsai, X.-S. Xie, and D. K. Stone Identification of a 14-kDa Subunit Associated with the Catalytic Sector of Clathrin-coated Vesicle H[IMAGE]-ATPase J. Biol. Chem., February 9, 1996; 271(6): 3324 - 3327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Liu, P. M. Kane, P. R. Newman, and M. Forgac Site-directed Mutagenesis of the Yeast V-ATPase B Subunit (Vma2p) J. Biol. Chem., January 26, 1996; 271(4): 2018 - 2022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-B. Peng and S. B. Peng Nucleotide Labeling and Reconstitution of the Recombinant 58-kDa Subunit of the Vacuolar Proton-translocating ATPase J. Biol. Chem., July 14, 1995; 270(28): 16926 - 16931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, E. Vasilyeva, Y. Feng, and M. Forgac J. Biol. Chem., June 30, 1995; 270(26): 15494 - 15500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Adessi, A Chapel, M Vincon, T Rabilloud, G Klein, M Satre, and J Garin Identification of major proteins associated with Dictyostelium discoideum endocytic vesicles J. Cell Sci., January 10, 1995; 108(10): 3331 - 3337. [Abstract] [PDF]
|
|
|
|
 |
|
 P. Kane, C. Yamashiro, D. Wolczyk, N Neff, M Goebl, and T. Stevens Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase Science, November 2, 1990; 250(4981): 651 - 657. [Abstract] [PDF]
|
|
|
|
|
|
C. Landolt-Marticorena, K. M. Williams, J. Correa, W. Chen, and M. F. Manolson Evidence That the NH2 Terminus of Vph1p, an Integral Subunit of the V0 Sector of the Yeast V-ATPase, Interacts Directly with the Vma1p and Vma13p Subunits of the V1 Sector J. Biol. Chem., May 12, 2000; 275(20): 15449 - 15457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Xu and M. Forgac Subunit D (Vma8p) of the Yeast Vacuolar H+-ATPase Plays a Role in Coupling of Proton Transport and ATP Hydrolysis J. Biol. Chem., July 14, 2000; 275(29): 22075 - 22081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kawasaki-Nishi, T. Nishi, and M. Forgac Yeast V-ATPase Complexes Containing Different Isoforms of the 100-kDa a-subunit Differ in Coupling Efficiency and in Vivo Dissociation J. Biol. Chem., May 18, 2001; 276(21): 17941 - 17948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Arata, J. D. Baleja, and M. Forgac Cysteine-directed Cross-linking to Subunit B Suggests That Subunit E Forms Part of the Peripheral Stalk of the Vacuolar H+-ATPase J. Biol. Chem., January 25, 2002; 277(5): 3357 - 3363. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
