JBC Avanti Polar Lipids

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J. Biol. Chem., Vol. 263, Issue 29, 14614-14616, Oct, 1988

mRNA-induced expression of the cardiac Na+-Ca2+ exchanger in Xenopus oocytes

E Sigel, R Baur, H Porzig and H Reuter
Institute of Pharmacology, University of Bern, Switzerland.

Xenopus oocytes were injected with total mRNA isolated from hearts of 1- day-old chicks. After 5 days of incubation the follicular cell layers were removed and the oocytes were loaded with Na+ by incubation in hypertonic EGTA solution at 37 degrees C. The Na+-loaded oocytes accumulated 45Ca2+ from a Na+-free medium at a 3-18-fold higher rate than noninjected oocytes or oocytes injected with control solution containing no mRNA. Oocytes not subjected to the Na+-loading procedure showed no mRNA-dependent 45Ca2+ uptake. Size fractionation of the mRNA using sucrose density gradient centrifugation under denaturing conditions led to the identification of a 25 S fraction competent for induction of the Na+-Ca2+ exchange system.
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