HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sung, Y. C. Articles by Fuchs, J. A. Search for Related Content PubMed PubMed Citation Articles by Sung, Y. C. Articles by Fuchs, J. A. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 263, Issue 29, 14769-14775, Oct, 1988

Characterization of the cyn operon in Escherichia coli K12

YC Sung and JA Fuchs
Department of Biochemistry, University of Minnesota, St. Paul 55108.

Escherichia coli can overcome the toxicity of environmental cyanate by hydrolysis of cyanate to ammonia and bicarbonate. This reaction is catalyzed by the enzyme cyanase, encoded by the cynS gene. The nucleotide sequence of cynS has been reported (Sung, Y.-c., Anderson, P. M., and Fuchs, J. A. (1987) J. Bacteriol. 169, 5224-5230). The nucleotide sequence of the complete cyn operon has now been determined. The cyn operon is approximately 2600 base pairs and includes cynT, cynS, and cynX, which encode cyanate permease, cyanase, and a protein of unknown function, respectively. Two cyanate-inducible transcripts of 1500 and 2500 nucleotides, respectively, were detected by Northern blot analysis. S1 nuclease mapping experiments indicated that two different cyn mRNAs have a common 5'-end and two different 3'-ends. One 3'-end was located within the coding region of cynX, whereas the other 3'-end includes the entire DNA sequence of cynX. The longer transcript contained 98 nucleotides complementary to lac mRNA produced by the predominant lac transcription termination sequence. Termination vectors were used to show that both 3'-ends were generated by sequences that caused transcriptional termination in vivo. Expression vectors were used to demonstrate that a protein corresponding to the expected size was synthesized from the DNA fragment containing the open reading frame designated cynX. The predicted amino acid sequence of cynX indicates that it is a very hydrophobic protein. The level of cynX expression was significantly less than that of cynT or cynS expression.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				V. M. Luque-Almagro, M.-J. Huertas, L. P. Saez, M. M. Luque-Romero, C. Moreno-Vivian, F. Castillo, M. D. Roldan, and R. Blasco Characterization of the Pseudomonas pseudoalcaligenes CECT5344 Cyanase, an Enzyme That Is Not Essential for Cyanide Assimilation Appl. Envir. Microbiol., October 15, 2008; 74(20): 6280 - 6288. [Abstract] [Full Text] [PDF]

				G. S. Espie, F. Jalali, T. Tong, N. J. Zacal, and A. K.-C. So Involvement of the cynABDS Operon and the CO2-Concentrating Mechanism in the Light-Dependent Transport and Metabolism of Cyanate by Cyanobacteria J. Bacteriol., February 1, 2007; 189(3): 1013 - 1024. [Abstract] [Full Text] [PDF]

				J. D. Cronk, J. A. Endrizzi, M. R. Cronk, J. W. O'neill, and K. Y.J. Zhang Crystal structure of E. coli {beta}-carbonic anhydrase, an enzyme with an unusual pH-dependent activity Protein Sci., May 1, 2001; 10(5): 911 - 922. [Abstract] [Full Text]

				M. K. B. Berlyn Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map Microbiol. Mol. Biol. Rev., September 1, 1998; 62(3): 814 - 984. [Abstract] [Full Text] [PDF]

				S. S. Pao, I. T. Paulsen, and M. H. Saier Jr. Major Facilitator Superfamily Microbiol. Mol. Biol. Rev., March 1, 1998; 62(1): 1 - 34. [Abstract] [Full Text] [PDF]

				S. Mitsuhashi and S. Miyachi Amino Acid Sequence Homology between N- and C-terminal Halves of a Carbonic Anhydrase in Porphyridium purpureum, as Deduced from the Cloned cDNA J. Biol. Chem., November 8, 1996; 271(45): 28703 - 28709. [Abstract] [Full Text] [PDF]