HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stern, L. Articles by Kunos, G. Search for Related Content PubMed PubMed Citation Articles by Stern, L. Articles by Kunos, G. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 263, Issue 31, 15876-15879, 11, 1988

Synergistic regulation of pulmonary beta-adrenergic receptors by glucocorticoids and interleukin-1

L Stern and G Kunos
Department of Pharmacology and Medicine, McGill University, Montreal, Canada.

Coculturing IM9 human lymphocytes and A549 human lung adenocarcinoma cells results in a 2-3-fold increase in the density of beta-adrenergic receptors in the latter, as quantified by 125I-cyanopindolol binding. Lymphocyte-conditioned medium (LCM) has the same effect, which is moderately sensitive to heat, is retained by ultrafiltration over a Mr 10,000 cut-off filter, and is reduced by trypsin treatment or by preincubation of lymphocytes with 0.3 micrograms/ml cycloheximide. Treatment of lung cells with cycloheximide also prevents the effect of LCM. Glucocorticoids, which also increase beta-receptor density in A549 cells, markedly potentiate the effect of LCM. Gel permeation high pressure liquid chromatography of LCM yields three peaks of biological activity with Mr 70,000, 35,000, and 15,000. Monocytic interleukin-1 (IL-1) mimics the effect of LCM in that it increases beta-receptor density in A549 cells (EC50 0.3 pM), and its effect is potentiated by cortisol. Recombinant IL-1 alpha is somewhat more potent than IL-1 beta, while interleukin-2 and interferon-alpha are ineffective. Tumor necrosis factor alpha causes a small increase in beta-receptors, which is not influenced by glucocorticoids. A polyclonal anti-IL-1 antibody inhibits the effect of IL-1 and the effect of the 15-kDa but not the 35- and 70-kDa fractions of LCM. The activity of the latter two fractions is also unaffected by anti-tumor necrosis factor alpha antibody. These results indicate that lymphocytes release protein factors including IL- 1 that up-regulate pulmonary beta-adrenergic receptors by an action that involves protein synthesis. The possible relevance of this regulatory mechanism for the pathomechanism of certain respiratory diseases is discussed.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T. Li, S. Varadarajulu, L. L. Beard, J. Yun, and H. G. Folkesson A Noninflammatory Interleukin-1beta Fragment Stimulates Fetal Lung Fluid Absorption in Guinea Pigs J. Pharmacol. Exp. Ther., February 1, 2007; 320(2): 877 - 884. [Abstract] [Full Text] [PDF]

				X. Ye, R. Acharya, J. B. Herbert, S. E. Hamilton, and H. G. Folkesson IL-1{beta} stimulates alveolar fluid absorption in fetal guinea pig lungs via the hypothalamus-pituitary-adrenal gland axis Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L756 - L766. [Abstract] [Full Text] [PDF]

				S. K. Lutgendorf, S. Cole, E. Costanzo, S. Bradley, J. Coffin, S. Jabbari, K. Rainwater, J. M. Ritchie, M. Yang, and A. K. Sood Stress-Related Mediators Stimulate Vascular Endothelial Growth Factor Secretion by Two Ovarian Cancer Cell Lines Clin. Cancer Res., October 1, 2003; 9(12): 4514 - 4521. [Abstract] [Full Text] [PDF]

				I. J. Elenkov, R. L. Wilder, G. P. Chrousos, and E. S. Vizi The Sympathetic Nerve---An Integrative Interface between Two Supersystems: The Brain and the Immune System Pharmacol. Rev., December 1, 2000; 52(4): 595 - 638. [Abstract] [Full Text] [PDF]

				K. E. Hadri, A. Courtalon, X. Gauthereau, A.-M. Chambaut-Guerin, J. Pairault, and B. Feve Differential Regulation by Tumor Necrosis Factor-alpha of beta 1-, beta 2-, and beta 3-Adrenoreceptor Gene Expression in 3T3-F442A Adipocytes J. Biol. Chem., September 26, 1997; 272(39): 24514 - 24521. [Abstract] [Full Text] [PDF]

				V. Dumasius, J. I. Sznajder, Z. S. Azzam, J. Boja, G. M. Mutlu, M. B. Maron, and P. Factor {beta}2-Adrenergic Receptor Overexpression Increases Alveolar Fluid Clearance and Responsiveness to Endogenous Catecholamines in Rats Circ. Res., November 9, 2001; 89(10): 907 - 914. [Abstract] [Full Text] [PDF]