| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 263, Issue 33, 17307-17311, Nov, 1988
SS Stojilkovic, JP Chang, D Ngo and KJ Catt
The role of protein kinase C in luteinizing hormone (LH) release was
analyzed in studies on the actions of phorbol esters and gonadotropin-
releasing hormone (GnRH) in normal and protein kinase C
(Ca2+/phospholipid-dependent enzyme)-depleted pituitary cell cultures. LH
secretory responses of normal pituitary cells to GnRH were reduced but not
abolished in Ca2+-deficient medium, consistent with the existence of
extracellular Ca2+-dependent and -independent components of GnRH action.
Both of these components could be elicited by treatment with
12-O-tetradecanoylphorbol 13-acetate (TPA). The LH secretory responses to
TPA and GnRH were additive only at low doses and converged to a common
maximum at high concentrations of the agonists in the presence or absence
of extracellular Ca2+. The release of stored LH by GnRH and TPA was
accompanied by secretion of newly synthesized LH from 2 to 5 h during
stimulation by either of the agonists. LH synthesis was increased in a
progressive and dose-dependent manner by GnRH and TPA, and the ratio
between newly synthesized and released hormone was near 1:2. TPA caused
rapid and complete translocation of cytosolic protein kinase C to the
particulate fraction of pituitary cells, followed by a progressive decrease
in total enzyme content to approximately 10% after 6 h. Partial recovery of
the cytosolic enzyme (to 20%) occurred after washing and reincubation for
15 h. Such kinase C-depleted cells showed prominent, dose-dependent
reductions in the actions of GnRH and TPA on LH release and synthesis in
both normal and Ca2+-deficient media. These observations support the
hypothesis that protein kinase C participates in LH biosynthesis and
secretion in pituitary gonadotrophs and is involved in the actions of GnRH
upon these processes.
Evidence for a role of protein kinase C in luteinizing hormone synthesis and secretion. Impaired responses to gonadotropin-releasing hormone in protein kinase C-depleted pituitary cells
Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20982.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. K. Cheng and P. C. K. Leung Molecular Biology of Gonadotropin-Releasing Hormone (GnRH)-I, GnRH-II, and Their Receptors in Humans Endocr. Rev., April 1, 2005; 26(2): 283 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Jorgensen, C. C. Quirk, and J. H. Nilson Multiple and Overlapping Combinatorial Codes Orchestrate Hormonal Responsiveness and Dictate Cell-Specific Expression of the Genes Encoding Luteinizing Hormone Endocr. Rev., August 1, 2004; 25(4): 521 - 542. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Junoy, H. Maccario, J.-L. Mas, A. Enjalbert, and S. V. Drouva Proteasome Implication in Phorbol Ester- and GnRH-Induced Selective Down-Regulation of PKC ({alpha}, {epsilon}, {zeta}) in {alpha}T3-1 and L{beta}T2 Gonadotrope Cell Lines Endocrinology, April 1, 2002; 143(4): 1386 - 1403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Roberson, T. Zhang, H. L. Li, and J. M. Mulvaney Activation of the p38 Mitogen-Activated Protein Kinase Pathway by Gonadotropin-Releasing Hormone Endocrinology, March 1, 1999; 140(3): 1310 - 1318. [Abstract] [Full Text]
|
|
|
|
|
|
C. Rabadan-Diehl and G. Aguilera Glucocorticoids Increase Vasopressin V1b Receptor Coupling to Phospholipase C Endocrinology, July 1, 1998; 139(7): 3220 - 3226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Saunders, E. Sabbagh, W. W. Chin, and U. B. Kaiser Differential Use of Signal Transduction Pathways in the Gonadotropin-Releasing Hormone-Mediated Regulation of Gonadotropin Subunit Gene Expression Endocrinology, April 1, 1998; 139(4): 1835 - 1843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Billiard, D.-S. Koh, D. F. Babcock, and B. Hille Protein kinase C as a signal for exocytosis PNAS, October 28, 1997; 94(22): 12192 - 12197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Harris, N. Reiss, and Z. Naor Differential Activation of Protein Kinase C delta and epsilon Gene Expression by Gonadotropin-releasing Hormone in alpha T3-1 Cells. AUTOREGULATION BY PROTEIN KINASE C J. Biol. Chem., May 23, 1997; 272(21): 13534 - 13540. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
