HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Crabb, J. W. Articles by Saari, J. C. Search for Related Content PubMed PubMed Citation Articles by Crabb, J. W. Articles by Saari, J. C. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 263, Issue 35, 18688-18692, 12, 1988

Cloning of the cDNAs encoding the cellular retinaldehyde-binding protein from bovine and human retina and comparison of the protein structures

JW Crabb, S Goldflam, SE Harris and JC Saari
W. Alton Jones Cell Science Center, Inc., Lake Placid, New York 12946.

A 1173-base pair cDNA encoding bovine cellular retinaldehyde-binding protein (CRALBP) was cloned from a bovine retinal cDNA expression library using as probes both anti-CRALBP polyclonal and monoclonal antibodies. The amino acid sequence deduced from the cDNA corresponds exactly to that determined by direct analysis of NH2-terminally acetylated bovine CRALBP (Crabb, J. W., Johnson, C. M., Carr, S. A., Armes, L. G., and Saari, J. C. (1988) J. Biol. Chem. 263, 18678-18687). Nick-translated bovine CRALBP cDNA probes were then used to clone from a human retinal cDNA library a 1317-base pair cDNA encoding human CRALBP. Bovine and human CRALBP are 92% identical in amino acid sequence and not related to any other known protein sequence. Both the bovine and human proteins contain 316 residues and have calculated molecular weights of 36,378 and 36,347, respectively, exclusive of the NH2-terminal blocking groups. The CRALBP cDNA clones should prove valuable as tools for studying the physiological role of the protein in vision and visual disorders.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				E. V. Kostenko, G. M. Mahon, L. Cheng, and I. P. Whitehead The Sec14 Homology Domain Regulates the Cellular Distribution and Transforming Activity of the Rho-specific Guanine Nucleotide Exchange Factor Dbs J. Biol. Chem., January 28, 2005; 280(4): 2807 - 2817. [Abstract] [Full Text] [PDF]

				C. S. Alge, S. Suppmann, S. G. Priglinger, A. S. Neubauer, C. A. May, S. Hauck, U. Welge-Lussen, M. Ueffing, and A. Kampik Comparative Proteome Analysis of Native Differentiated and Cultured Dedifferentiated Human RPE Cells Invest. Ophthalmol. Vis. Sci., August 1, 2003; 44(8): 3629 - 3641. [Abstract] [Full Text] [PDF]

				M. Alizadeh, M. Wada, C. M. Gelfman, J. T. Handa, and L. M. Hjelmeland Downregulation of Differentiation Specific Gene Expression by Oxidative Stress in ARPE-19 Cells Invest. Ophthalmol. Vis. Sci., October 1, 2001; 42(11): 2706 - 2713. [Abstract] [Full Text] [PDF]

				N. Shibata, M. Arita, Y. Misaki, N. Dohmae, K. Takio, T. Ono, K. Inoue, and H. Arai Supernatant protein factor, which stimulates the conversion of squalene to lanosterol, is a cytosolic squalene transfer protein and enhances cholesterol biosynthesis PNAS, February 27, 2001; 98(5): 2244 - 2249. [Abstract] [Full Text] [PDF]

				J. W. Crabb, Z. Nie, Y. Chen, J. D. Hulmes, K. A. West, J. T. Kapron, S. E. Ruuska, N. Noy, and J. C. Saari Cellular Retinaldehyde-binding Protein Ligand Interactions. GLN-210 AND LYS-221 ARE IN THE RETINOID BINDING POCKET J. Biol. Chem., August 14, 1998; 273(33): 20712 - 20720. [Abstract] [Full Text] [PDF]

				B. N. Kennedy, S. Goldflam, M. A. Chang, P. Campochiaro, A. A. Davis, D. J. Zack, and J. W. Crabb Transcriptional Regulation of Cellular Retinaldehyde-binding Protein in the Retinal Pigment Epithelium. A ROLE FOR THE PHOTORECEPTOR CONSENSUS ELEMENT J. Biol. Chem., March 6, 1998; 273(10): 5591 - 5598. [Abstract] [Full Text] [PDF]

				R Blomhoff, M. Green, T Berg, and K. Norum Transport and storage of vitamin A Science, October 19, 1990; 250(4979): 399 - 404. [Abstract] [PDF]

				J. M. Kruger, T. Fukushima, V. Cherepanov, N. Borregaard, C. Loeve, C. Shek, K. Sharma, A. K. Tanswell, C.-W. Chow, and G. P. Downey Protein-tyrosine Phosphatase MEG2 Is Expressed by Human Neutrophils. LOCALIZATION TO THE PHAGOSOME AND ACTIVATION BY POLYPHOSPHOINOSITIDES J. Biol. Chem., January 18, 2002; 277(4): 2620 - 2628. [Abstract] [Full Text] [PDF]