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J. Biol. Chem., Vol. 263, Issue 35, 18834-18841, 12, 1988
LL Muldoon, KD Rodland and BE Magun
Transforming growth factor type beta (TGF beta) alters the cellular
response to epidermal growth factor (EGF) for a variety of processes
ranging from early transport activities and gene transcription to
mitogenesis. In order to test the hypothesis that altered signal
transduction mechanisms may mediate both the transforming effects of TGF
beta and the modulation of EGF-stimulated processes by TGF beta, we have
examined second messenger levels in response to growth factor treatment.
The addition of EGF or prolonged treatment with TGF beta increased the rate
of 45Ca influx in serum-deprived, confluent Rat-1 cells, while the addition
of EGF to TGF beta-pretreated cells produced an additive increase in Ca2+
influx. The stimulation of Ca2+ influx by TGF beta was only observed at
incubation times greater than 1 h and was inhibited by inclusion of
actinomycin D, suggesting that a newly transcribed gene product was
required for the observed response to TGF beta. Both EGF and TGF beta
displayed similar time and concentration dependencies for stimulation of
Ca2+ influx and for accumulation of inositol trisphosphate (IP3). The
increase in IP3 accumulation in response to either EGF or TGF beta required
the presence of extracellular Ca2+, and the observed concentration
dependencies were similar for the stimulation of phosphatidylinositol
turnover and Ca2+ influx. The EGF- and TGF beta-stimulated increases in
Ca2+ influx could be blocked by cobalt, cadmium, and
[ethylenebis(oxyethylenenitrilo)] tetraacetic acid, but not by specific
Ca2+ channel blockers such as nifedipine or verapamil, suggesting that
these growth factors do not act via L-type voltage-sensitive calcium
channels. Those calcium blockers which inhibited Ca2+ influx also inhibited
inositol phosphate release. These data, taken together, indicate that Ca2+
influx and inositol phosphate release are coupled in Rat-1 cells and
suggest that influx of Ca2+ from the extracellular medium is responsible
for the changes in IP3 accumulation observed in response to both EGF and
TGF beta.
Transforming growth factor beta and epidermal growth factor alter calcium influx and phosphatidylinositol turnover in rat-1 fibroblasts
Oregon Health Sciences University, Portland 97201.
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