J. Biol. Chem., Vol. 263, Issue 36, 19278-19281, Dec, 1988
Crystallographic study of the complex between sulfite and bakers' yeast flavocytochrome b2
M Tegoni and FS Mathews
Centre de Genetique Moleculaire du Centre National de la Recherche Scientifique, Gif sur Yvette, France.
The complex between Saccharomyces cerevisiae flavocytochrome b2 and the
sulfite anion has been analyzed by x-ray diffraction. A map of the
difference in electron density between the complex and the native protein
has been computed. One positive peak of electron density is visible at the
active site of each of the two subunits in the asymmetric unit, very close
to the N-5 of the flavin. The molecular fragment SO3(2-) can account for
the shape of this difference in electron density. A third peak is visible
in the subunit containing pyruvate, the reaction product. It is a peak of
negative electron density localized at the position where the pyruvate
usually is in the native form. These results are interpreted on the basis
of the mechanism defined in solution for the reaction between flavins and
sulfite.
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