HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Konrad, M. Search for Related Content PubMed PubMed Citation Articles by Konrad, M. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 263, Issue 36, 19468-19474, 12, 1988

Analysis and in vivo disruption of the gene coding for adenylate kinase (ADK1) in the yeast Saccharomyces cerevisiae

M Konrad
Department of Molecular Genetics, Max-Planck-Institute for Biophysical Chemistry, Gottingen, West Germany.

The gene (designated ADK1) encoding the so-called cytosolic adenylate kinase of the yeast Saccharomyces cerevisiae was isolated using a single mixed oligonucleotide hybridization probe designed from the published amino acid sequence. ADK1 was found to be identical to an adenylate kinase gene recently isolated by an approach entirely different from ours (Magdolen, V., Oechsner, U., and Bandlow, W. (1987) Curr. Genet. 12, 405-411). The gene resides on yeast chromosome IV adjacent to the histone gene H2A-1. Southern blot analysis revealed only one copy of the gene, and no other related yeast DNA sequences were detected. By gene disruption it is shown that the ADK1 gene is needed for normal cell proliferation but is not essential for cell viability. Immunological studies confirmed the absence of the ADK1 gene product in mutant cells; in extracts of total cellular protein, however, there were still about 10% of the wild-type enzymatic activity present. This indicates the existence of two or more adenylate kinase isozymes in yeast. From preliminary 31P NMR measurements on suspensions of yeast cells, a significant decrease in the level of nucleoside triphosphates was found in the mutant strain carrying the disrupted and partially deleted ADK1 locus.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				W. Cao, L. Haig-Ladewig, G. L. Gerton, and S. B. Moss Adenylate Kinases 1 and 2 Are Part of the Accessory Structures in the Mouse Sperm Flagellum Biol Reprod, October 1, 2006; 75(4): 492 - 500. [Abstract] [Full Text] [PDF]

				L. Mateos, A. Jimenez, J. L. Revuelta, and M. A. Santos Purine Biosynthesis, Riboflavin Production, and Trophic-Phase Span Are Controlled by a Myb-Related Transcription Factor in the Fungus Ashbya gossypii. Appl. Envir. Microbiol., July 1, 2006; 72(7): 5052 - 5060. [Abstract] [Full Text] [PDF]

				A. Zuzuarregui, L. Monteoliva, C. Gil, and M.{m. d.}l. del Olmo Transcriptomic and Proteomic Approach for Understanding the Molecular Basis of Adaptation of Saccharomyces cerevisiae to Wine Fermentation Appl. Envir. Microbiol., January 1, 2006; 72(1): 836 - 847. [Abstract] [Full Text] [PDF]

				S. Huang and E. K. O'Shea A Systematic High-Throughput Screen of a Yeast Deletion Collection for Mutants Defective in PHO5 Regulation Genetics, April 1, 2005; 169(4): 1859 - 1871. [Abstract] [Full Text] [PDF]

				M. L. Ginger, E. S. Ngazoa, C. A. Pereira, T. J. Pullen, M. Kabiri, K. Becker, K. Gull, and D. Steverding Intracellular Positioning of Isoforms Explains an Unusually Large Adenylate Kinase Gene Family in the Parasite Trypanosoma brucei J. Biol. Chem., March 25, 2005; 280(12): 11781 - 11789. [Abstract] [Full Text] [PDF]