HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Seufert, W. Articles by Messer, W. Search for Related Content PubMed PubMed Citation Articles by Seufert, W. Articles by Messer, W. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 263, Issue 6, 2719-2723, 02, 1988

Functionality of the dnaA protein binding site in DNA replication is orientation-dependent

W Seufert, B Dobrinski, R Lurz and W Messer
Max-Planck-Institut fur Molekulare Genetik, Berlin, West Germany.

We analyzed the functionality of different dnaA protein binding sites by assaying in vitro dnaA-dependent replication of pBR322 derivatives. Single dnaA sites from oriC and from the mioC and dnaA gene promoters were active when combined with the primer generating element of pBR322 in a proper distance. Prereplisome assembly did not require sequences in addition to the 9-base pair consensus dnaA binding site. Inversion of the structurally asymmetric dnaA site relative to its orientation in wild type pBR322 resulted in a marked reduction in replication efficiency, as observed with five different dnaA sites studied. The direction of DNA replication was not affected.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Sun, M. Zhou, Y. Li, and H. Xiang Molecular Characterization of the Minimal Replicon and the Unidirectional Theta Replication of pSCM201 in Extremely Halophilic Archaea J. Bacteriol., December 1, 2006; 188(23): 8136 - 8144. [Abstract] [Full Text] [PDF]

				E. Viguera, P. Hernandez, D. B. Krimer, R. Lurz, and J. B. Schvartzman Visualisation of plasmid replication intermediates containing reversed forks Nucleic Acids Res., January 15, 2000; 28(2): 498 - 503. [Abstract] [Full Text] [PDF]

				G. del Solar, R. Giraldo, M. J. Ruiz-Echevarria, M. Espinosa, and R. Diaz-Orejas Replication and Control of Circular Bacterial Plasmids Microbiol. Mol. Biol. Rev., June 1, 1998; 62(2): 434 - 464. [Abstract] [Full Text] [PDF]

				E. Viguera, P. Hernandez, D. B. Krimer, A. S. Boistov, R. Lurz, J. C. Alonso, and J. B. Schvartzman The ColE1 Unidirectional Origin Acts as a Polar Replication Fork Pausing Site J. Biol. Chem., September 13, 1996; 271(37): 22414 - 22421. [Abstract] [Full Text] [PDF]