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J. Biol. Chem., Vol. 263, Issue 6, 2750-2755, Feb, 1988

CAMP-dependent protein kinase of sea urchin sperm phosphorylates sperm histone H1 on a single site

DC Porter, GW Moy and VD Vacquier
Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla 92093.

The phosphorylation of sperm specific histone H1 in the sea urchin Strongylocentrotus purpuratus occurs both in vivo and in vitro on a single serine site in the sequence Arg-Lys-Gly-Ser(P)-Ser-Asn-Ala-Arg. This is a preferred sequence for cAMP-dependent protein kinase. The in vitro phosphorylation is completely dependent on cAMP and is inhibited by the peptide protein kinase inhibitor. The protein kinase inhibitor H- 8 blocks the in vivo phosphorylation of H1 without damaging motility, the acrosome reaction or the ability of sperm to fuse with and activate eggs.
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