HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Morales, T. I. Articles by Hascall, V. C. Search for Related Content PubMed PubMed Citation Articles by Morales, T. I. Articles by Hascall, V. C. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 263, Issue 8, 3632-3638, Mar, 1988

Correlated metabolism of proteoglycans and hyaluronic acid in bovine cartilage organ cultures

TI Morales and VC Hascall
Bone Research Branch, National Institute of Dental Research, Bethesda, Maryland 20892.

Proteoglycans exist in cartilage as complexes in which many proteoglycan molecules are bound to a central filament of hyaluronic acid. Many studies have investigated changes taking place in proteoglycan monomer structure during cartilage catabolism usually under the assumption that hyaluronic acid is a relatively inert metabolic component of the complex. In this paper we present organ culture data supporting a new hypothesis that the catabolism of proteoglycans and hyaluronic acid are coordinately regulated by chondrocytes. The data indicates that: 1) newly synthesized hyaluronate and proteoglycan maintain a nearly constant ratio, almost identical to that existing for the total chemical amounts of these two components in cartilage tissue; 2) these two components are catabolized with virtually identical kinetics; and 3) this catabolic relationship in vitro reflects the loss of hyaluronate and proteoglycans from native, undissociated aggregates as isolated from the tissue. We conclude that hyaluronate catabolism is an integral part of the overall mechanism of proteoglycan resorption in cartilage and that further understanding of this process may be key to the elucidation of the regulatory pathways for proteoglycan resorption in health and disease.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Ohno, H.-J. Im, C. B. Knudson, and W. Knudson Hyaluronan Oligosaccharides Induce Matrix Metalloproteinase 13 via Transcriptional Activation of NF{kappa}B and p38 MAP Kinase in Articular Chondrocytes J. Biol. Chem., June 30, 2006; 281(26): 17952 - 17960. [Abstract] [Full Text] [PDF]

				W Hui, H E Barksby, D A Young, T E Cawston, N McKie, and A D Rowan Oncostatin M in combination with tumour necrosis factor {alpha} induces a chondrocyte membrane associated aggrecanase that is distinct from ADAMTS aggrecanase-1 or -2 Ann Rheum Dis, November 1, 2005; 64(11): 1624 - 1632. [Abstract] [Full Text] [PDF]

				G. Chow and W. Knudson Characterization of Promoter Elements of the Human HYAL-2 Gene J. Biol. Chem., July 22, 2005; 280(29): 26904 - 26912. [Abstract] [Full Text] [PDF]

				M. Ohno-Nakahara, K. Honda, K. Tanimoto, N. Tanaka, T. Doi, A. Suzuki, K. Yoneno, Y. Nakatani, M. Ueki, S. Ohno, et al. Induction of CD44 and MMP Expression by Hyaluronidase Treatment of Articular Chondrocytes J. Biochem., May 1, 2004; 135(5): 567 - 575. [Abstract] [Full Text] [PDF]

				M. I. Tammi, A. J. Day, and E. A. Turley Hyaluronan and Homeostasis: A Balancing Act J. Biol. Chem., February 8, 2002; 277(7): 4581 - 4584. [Full Text] [PDF]

				A. Calabro, V. C. Hascall, and R. J. Midura Adaptation of FACE methodology for microanalysis of total hyaluronan and chondroitin sulfate composition from cartilage Glycobiology, March 1, 2000; 10(3): 283 - 293. [Abstract] [Full Text] [PDF]

				A. H. K. Plaas, L. A. West, S. Wong-Palms, and F. R. T. Nelson Glycosaminoglycan Sulfation in Human Osteoarthritis. DISEASE-RELATED ALTERATIONS AT THE NON-REDUCING TERMINI OF CHONDROITIN AND DERMATAN SULFATE J. Biol. Chem., May 15, 1998; 273(20): 12642 - 12649. [Abstract] [Full Text] [PDF]

				P. H. Weigel, V. C. Hascall, and M. Tammi Hyaluronan Synthases J. Biol. Chem., May 30, 1997; 272(22): 13997 - 14000. [Full Text] [PDF]

				A. Rossi, J. Bonaventure, A.-L. Delezoide, G. Cetta, and A. Superti-Furga Undersulfation of Proteoglycans Synthesized by Chondrocytes from a Patient with Achondrogenesis Type 1B Homozygous for an L483P Substitution in the Diastrophic Dysplasia Sulfate Transporter J. Biol. Chem., August 2, 1996; 271(31): 18456 - 18464. [Abstract] [Full Text] [PDF]

				G. Chow, C. B. Knudson, G. Homandberg, and W. Knudson Increased Expression of CD44 in Bovine Articular Chondrocytes by Catabolic Cellular Mediators J. Biol. Chem., November 17, 1995; 270(46): 27734 - 27741. [Abstract] [Full Text] [PDF]

				M. W. Lark, J. T. Gordy, J. R. Weidner, J. Ayala, J. H. Kimura, H. R. Williams, R. A. Mumford, C. R. Flannery, S. S. Carlson, M. Iwata, et al. Cell-mediated Catabolism of Aggrecan J. Biol. Chem., February 10, 1995; 270(6): 2550 - 2556. [Abstract] [Full Text] [PDF]

				Q Hua, C. Knudson, and W Knudson Internalization of hyaluronan by chondrocytes occurs via receptor-mediated endocytosis J. Cell Sci., January 9, 1993; 106(1): 365 - 375. [Abstract] [PDF]