Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blanco, L.
Right arrow Articles by Salas, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blanco, L.
Right arrow Articles by Salas, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J. Biol. Chem., Vol. 264, Issue 15, 8935-8940, 05, 1989

Highly efficient DNA synthesis by the phage phi 29 DNA polymerase. Symmetrical mode of DNA replication

L Blanco, A Bernad, JM Lazaro, G Martin, C Garmendia and M Salas
Centro de Biologia Molecular (Consejo Superior de Investigaciones Cientificas), Universidad Autonoma de Madrid, Spain.

The results presented in this paper indicate that the phi 29 DNA polymerase is the only enzyme required for efficient synthesis of full length phi 29 DNA with the phi 29 terminal protein, the initiation primer, as the only additional protein requirement. Analysis of phi 29 DNA polymerase activity in various in vitro DNA replication systems indicates that two main reasons are responsible for the efficiency of this minimal system: 1) the phi 29 DNA polymerase is highly processive in the absence of any accessory protein; 2) the polymerase itself is able to produce strand displacement coupled to the polymerization process. Using primed M13 DNA as template, the phi 29 DNA polymerase is able to synthesize DNA chains greater than 70 kilobase pairs. Furthermore, conditions that increase the stability of secondary structure in the template do not affect the processivity and strand displacement ability of the enzyme. Thus, the catalytic properties of the phi 29 DNA polymerase are appropriate for a phi 29 DNA replication mechanism involving two replication origins, strand displacement and continuous synthesis of both strands. The enzymology of phi 29 DNA replication would support a symmetrical model of DNA replication.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
T. F. F. Ng, C. Manire, K. Borrowman, T. Langer, L. Ehrhart, and M. Breitbart
Discovery of a Novel Single-Stranded DNA Virus from a Sea Turtle Fibropapilloma by Using Viral Metagenomics
J. Virol., March 15, 2009; 83(6): 2500 - 2509.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
J. Huang, J. Pang, T. Watanabe, H.-K. Ng, and H. Ohgaki
Whole Genome Amplification for Array Comparative Genomic Hybridization Using DNA Extracted from Formalin-Fixed, Paraffin-Embedded Histological Sections
J. Mol. Diagn., March 1, 2009; 11(2): 109 - 116.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
J. Eid, A. Fehr, J. Gray, K. Luong, J. Lyle, G. Otto, P. Peluso, D. Rank, P. Baybayan, B. Bettman, et al.
Real-Time DNA Sequencing from Single Polymerase Molecules
Science, January 2, 2009; 323(5910): 133 - 138.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. Rodriguez, J. M. Lazaro, M. Salas, and M. de Vega
Involvement of the TPR2 subdomain movement in the activities of {phi}29 DNA polymerase
Nucleic Acids Res., January 1, 2009; 37(1): 193 - 203.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Longas, L. Villar, J. M. Lazaro, M. de Vega, and M. Salas
Phage {varphi}29 and Nf terminal protein-priming domain specifies the internal template nucleotide to initiate DNA replication
PNAS, November 25, 2008; 105(47): 18290 - 18295.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
S. Margeridon, S. Carrouee-Durantel, I. Chemin, L. Barraud, F. Zoulim, C. Trepo, and A. Kay
Rolling Circle Amplification, a Powerful Tool for Genetic and Functional Studies of Complete Hepatitis B Virus Genomes from Low-Level Infections and for Directly Probing Covalently Closed Circular DNA
Antimicrob. Agents Chemother., September 1, 2008; 52(9): 3068 - 3073.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. Li, H.-J. Kim, C. Zheng, W. H. A. Chow, J. Lim, B. Keenan, X. Pan, B. Lemieux, and H. Kong
Primase-based whole genome amplification
Nucleic Acids Res., August 1, 2008; 36(13): e79 - e79.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
J. E. Torres-Munoz, M. Nunez, and C. K. Petito
Successful Application of Hyperbranched Multidisplacement Genomic Amplification to Detect HIV-1 Sequences in Single Neurons Removed from Autopsy Brain Sections by Laser Capture Microdissection
J. Mol. Diagn., July 1, 2008; 10(4): 317 - 324.
[Abstract] [Full Text] [PDF]

Home page
 Int J EpidemiolHome page
M. Amoli, D Carthy, H Platt, and W. Ollier
EBV Immortalization of human B lymphocytes separated from small volumes of cryo-preserved whole blood
Int. J. Epidemiol., April 1, 2008; 37(suppl_1): i41 - i45.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. J. Osborne and C. A. Thornton
Cell-free cloning of highly expanded CTG repeats by amplification of dimerized expanded repeats
Nucleic Acids Res., March 27, 2008; 36(4): e24 - e24.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. J. Fullwood, J. J. S. Tan, P. W. P. Ng, K. P. Chiu, J. Liu, C. L. Wei, and Y. Ruan
The use of multiple displacement amplification to amplify complex DNA libraries
Nucleic Acids Res., March 1, 2008; 36(5): e32 - e32.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Korlach, P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner
Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures
PNAS, January 29, 2008; 105(4): 1176 - 1181.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. Perez-Arnaiz, E. Longas, L. Villar, J. M. Lazaro, M. Salas, and M. de Vega
Involvement of phage {phi}29 DNA polymerase and terminal protein subdomains in conferring specificity during initiation of protein-primed DNA replication
Nucleic Acids Res., December 18, 2007; 35(21): 7061 - 7073.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. d. Vega and M. Salas
A highly conserved Tyrosine residue of family B DNA polymerases contributes to dictate translesion synthesis past 8-oxo-7,8-dihydro-2'-deoxyguanosine
Nucleic Acids Res., August 1, 2007; (2007) gkm545v2.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. Longas, M. de Vega, J. M. Lazaro, and M. Salas
Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity
Nucleic Acids Res., November 6, 2006; 34(20): 6051 - 6063.
[Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. Perez-Arnaiz, J. M. Lazaro, M. Salas, and M. de Vega
Involvement of {varphi}29 DNA polymerase thumb subdomain in the proper coordination of synthesis and degradation during DNA replication
Nucleic Acids Res., June 6, 2006; 34(10): 3107 - 3115.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
C. B. Abulencia, D. L. Wyborski, J. A. Garcia, M. Podar, W. Chen, S. H. Chang, H. W. Chang, D. Watson, E. L. Brodie, T. C. Hazen, et al.
Environmental whole-genome amplification to access microbial populations in contaminated sediments.
Appl. Envir. Microbiol., May 1, 2006; 72(5): 3291 - 3301.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Serrano-Heras, M. Salas, and A. Bravo
A Uracil-DNA Glycosylase Inhibitor Encoded by a Non-uracil Containing Viral DNA
J. Biol. Chem., March 17, 2006; 281(11): 7068 - 7074.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. J. Weisenberger, M. Campan, T. I. Long, M. Kim, C. Woods, E. Fiala, M. Ehrlich, and P. W. Laird
Analysis of repetitive element DNA methylation by MethyLight
Nucleic Acids Res., December 2, 2005; 33(21): 6823 - 6836.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. A. Hutchison III, H. O. Smith, C. Pfannkoch, and J. C. Venter
Cell-free cloning using {phi}29 DNA polymerase
PNAS, November 29, 2005; 102(48): 17332 - 17336.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
P. Mavingui, V. Tran Van, E. Labeyrie, E. Rances, F. Vavre, and P. Simonet
Efficient Procedure for Purification of Obligate Intracellular Wolbachia pipientis and Representative Amplification of Its Genome by Multiple-Displacement Amplification
Appl. Envir. Microbiol., November 1, 2005; 71(11): 6910 - 6917.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
A. D. Short, L. J. Kennedy, O. Forman, A. Barnes, N. Fretwell, R. Wiggall, W. Thomson, and W. E. R. Ollier
Canine DNA Subjected to Whole Genome Amplification is Suitable for a Wide Range of Molecular Applications
J. Hered., November 1, 2005; 96(7): 829 - 835.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Z. Jiang, X. Zhang, R. Deka, and L. Jin
Genome amplification of single sperm using multiple displacement amplification
Nucleic Acids Res., June 7, 2005; 33(10): e91 - e91.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
I. Rodriguez, J. M. Lazaro, L. Blanco, S. Kamtekar, A. J. Berman, J. Wang, T. A. Steitz, M. Salas, and M. de Vega
A specific subdomain in {phi}29 DNA polymerase confers both processivity and strand-displacement capacity
PNAS, May 3, 2005; 102(18): 6407 - 6412.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
M. Bredel, C. Bredel, D. Juric, Y. Kim, H. Vogel, G. R. Harsh, L. D. Recht, J. R. Pollack, and B. I. Sikic
Amplification of Whole Tumor Genomes and Gene-by-Gene Mapping of Genomic Aberrations from Limited Sources of Fresh-Frozen and Paraffin-Embedded DNA
J. Mol. Diagn., May 1, 2005; 7(2): 171 - 182.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
D. P. Erwin, I. K. Erickson, M. E. Delwiche, F. S. Colwell, J. L. Strap, and R. L. Crawford
Diversity of Oxygenase Genes from Methane- and Ammonia-Oxidizing Bacteria in the Eastern Snake River Plain Aquifer
Appl. Envir. Microbiol., April 1, 2005; 71(4): 2016 - 2025.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. G. Paez, M. Lin, R. Beroukhim, J. C. Lee, X. Zhao, D. J. Richter, S. Gabriel, P. Herman, H. Sasaki, D. Altshuler, et al.
Genome coverage and sequence fidelity of {phi}29 polymerase-based multiple strand displacement whole genome amplification
Nucleic Acids Res., May 18, 2004; 32(9): e71 - e71.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Dahl, J. Baner, M. Gullberg, M. Mendel-Hartvig, U. Landegren, and M. Nilsson
Circle-to-circle amplification for precise and sensitive DNA analysis
PNAS, March 30, 2004; 101(13): 4548 - 4553.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Lovmar, M. Fredriksson, U. Liljedahl, S. Sigurdsson, and A.-C. Syvanen
Quantitative evaluation by minisequencing and microarrays reveals accurate multiplexed SNP genotyping of whole genome amplified DNA
Nucleic Acids Res., November 1, 2003; 31(21): e129 - e129.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. E. Wellinger, P. Schar, and J. M. Sogo
Rad52-Independent Accumulation of Joint Circular Minichromosomes during S Phase in Saccharomyces cerevisiae
Mol. Cell. Biol., September 15, 2003; 23(18): 6363 - 6372.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Truniger, J. M. Lazaro, M. de Vega, L. Blanco, and M. Salas
{phi}29 DNA Polymerase Residue Leu384, Highly Conserved in Motif B of Eukaryotic Type DNA Replicases, Is Involved in Nucleotide Insertion Fidelity
J. Biol. Chem., August 29, 2003; 278(35): 33482 - 33491.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. E. Johnson and C. C. Richardson
A Covalent Linkage between the Gene 5 DNA Polymerase of Bacteriophage T7 and Escherichia coli Thioredoxin, the Processivity Factor: FATE OF THIOREDOXIN DURING DNA SYNTHESIS
J. Biol. Chem., June 20, 2003; 278(26): 23762 - 23772.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
S. Hosono, A. F. Faruqi, F. B. Dean, Y. Du, Z. Sun, X. Wu, J. Du, S. F. Kingsmore, M. Egholm, and R. S. Lasken
Unbiased Whole-Genome Amplification Directly From Clinical Samples
Genome Res., May 1, 2003; 13(5): 954 - 964.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Bibillo and T. H. Eickbush
High Processivity of the Reverse Transcriptase from a Non-long Terminal Repeat Retrotransposon
J. Biol. Chem., September 13, 2002; 277(38): 34836 - 34845.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. B. Dean, S. Hosono, L. Fang, X. Wu, A. F. Faruqi, P. Bray-Ward, Z. Sun, Q. Zong, Y. Du, J. Du, et al.
Comprehensive human genome amplification using multiple displacement amplification
PNAS, April 16, 2002; 99(8): 5261 - 5266.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. Eisenbrandt, J. M. Lazaro, M. Salas, and M. d. Vega
{Phi}29 DNA polymerase residues Tyr59, His61 and Phe69 of the highly conserved ExoII motif are essential for interaction with the terminal protein
Nucleic Acids Res., March 15, 2002; 30(6): 1379 - 1386.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Serna-Rico, M. Salas, and W. J. J. Meijer
The Bacillus subtilis Phage phi 29 Protein p16.7, Involved in phi 29 DNA Replication, Is a Membrane-localized Single-stranded DNA-binding Protein
J. Biol. Chem., February 15, 2002; 277(8): 6733 - 6742.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
X. Qi, S. Bakht, K. M. Devos, M. D. Gale, and A. Osbourn
L-RCA (ligation-rolling circle amplification): a general method for genotyping of single nucleotide polymorphisms (SNPs)
Nucleic Acids Res., November 15, 2001; 29(22): e116 - e116.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
W. J. J. Meijer, J. A. Horcajadas, and M. Salas
{phi}29 Family of Phages
Microbiol. Mol. Biol. Rev., June 1, 2001; 65(2): 261 - 287.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
F. B. Dean, J. R. Nelson, T. L. Giesler, and R. S. Lasken
Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification
Genome Res., June 1, 2001; 11(6): 1095 - 1099.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Canceill, E. Viguera, and S. D. Ehrlich
Replication Slippage of Different DNA Polymerases Is Inversely Related to Their Strand Displacement Efficiency
J. Biol. Chem., September 24, 1999; 274(39): 27481 - 27490.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
V. V. McDougal and L. A. Guarino
Autographa californica Nuclear Polyhedrosis Virus DNA Polymerase: Measurements of Processivity and Strand Displacement
J. Virol., June 1, 1999; 73(6): 4908 - 4918.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
T. Pfister and E. Wimmer
Characterization of the Nucleoside Triphosphatase Activity of Poliovirus Protein 2C Reveals a Mechanism by Which Guanidine Inhibits Poliovirus Replication
J. Biol. Chem., March 12, 1999; 274(11): 6992 - 7001.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. de Vega, L. Blanco, and M. Salas
o29 DNA Polymerase Residue Ser122, a Single-stranded DNA Ligand for 3'-5' Exonucleolysis, Is Required to Interact with the Terminal Protein
J. Biol. Chem., October 30, 1998; 273(44): 28966 - 28977.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X.-M. Yang and C. C. Richardson
Amino Acid Changes in a Unique Sequence of Bacteriophage T7 DNA Polymerase Alter the Processivity of Nucleotide Polymerization
J. Biol. Chem., March 7, 1997; 272(10): 6599 - 6606.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Blanco and M. Salas
Relating Structure to Function in [IMAGE]29 DNA Polymerase
J. Biol. Chem., April 12, 1996; 271(15): 8509 - 8512.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1989 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement