| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 264, Issue 25, 14621-14623, Sep, 1989
Y Hodges-Garcia, PJ Hagerman and DE Pettijohn
The histone-like protein HU serves as an accessory factor that can
facilitate the interaction of certain proteins with their specific DNA
binding sites. Examples occur in different systems for prokaryotic DNA
replication, transcription, and gene regulation. The protein-DNA
interactions that are stimulated by HU generally involve coiling or looping
of the DNA, and the possibility has been considered that HU exerts its
effect by contributing flexibility to different DNA binding sites, but
there has been no direct demonstration of this. To explore the possibility
that HU can mediate tight DNA curvatures, we studied its effect on the
formation of DNA circles when DNA ligase cyclizes short linear DNA
fragments. It is demonstrated that HU greatly increases the cyclization
rates of all fragments that were examined having lengths greater than 98
base pairs. Fragments of 99, 108, 120, or 126 base pairs could not cyclize
in the absence of HU, but cyclization went rapidly with HU, showing that HU
can mediate very tight DNA curvatures.
DNA ring closure mediated by protein HU
Department of Biochemistry, Biophysics/Genetics, University of Colorado Health Sciences Center, Denver 80262.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. V. S. R. Ram, R. Naik, M. Ganguli, and S. Habib DNA organization by the apicoplast-targeted bacterial histone-like protein of Plasmodium falciparum Nucleic Acids Res., September 1, 2008; 36(15): 5061 - 5073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Becker, J. D. Kahn, and L. J. Maher III Eukaryotic HMGB proteins as replacements for HU in E. coli repression loop formation Nucleic Acids Res., July 1, 2008; 36(12): 4009 - 4021. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chatterjee, A. Basu, A. Basu, and S. K. Das Gupta DNA Bending in the Mycobacterial Plasmid pAL5000 Origin-RepB Complex J. Bacteriol., December 1, 2007; 189(23): 8584 - 8592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-H. Chan and J. T. Y. Wong Concentration-dependent organization of DNA by the dinoflagellate histone-like protein HCc3 Nucleic Acids Res., April 4, 2007; (2007) gkm165v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kar, E. J. Choi, F. Guo, E. K. Dimitriadis, S. L. Kotova, and S. Adhya Right-handed DNA Supercoiling by an Octameric Form of Histone-like Protein HU: MODULATION OF CELLULAR TRANSCRIPTION J. Biol. Chem., December 29, 2006; 281(52): 40144 - 40153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kamau, K. T. Bauerle, and A. Grove The Saccharomyces cerevisiae High Mobility Group Box Protein HMO1 Contains Two Functional DNA Binding Domains J. Biol. Chem., December 31, 2004; 279(53): 55234 - 55240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. van Noort, S. Verbrugge, N. Goosen, C. Dekker, and R. T. Dame Dual architectural roles of HU: Formation of flexible hinges and rigid filaments PNAS, May 4, 2004; 101(18): 6969 - 6974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Arthanari, K. Wojtuszewski, I. Mukerji, and P. H. Bolton Effects of HU Binding on the Equilibrium Cyclization of Mismatched, Curved, and Normal DNA Biophys. J., March 1, 2004; 86(3): 1625 - 1631. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hashimoto, B. Imhoff, M. M. Ali, and Y. W. Kow HU Protein of Escherichia coli Has a Role in the Repair of Closely Opposed Lesions in DNA J. Biol. Chem., August 1, 2003; 278(31): 28501 - 28507. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Y. Wong, D. C. New, J. C. W. Wong, and V. K. L. Hung Histone-Like Proteins of the Dinoflagellate Crypthecodinium cohnii Have Homologies to Bacterial DNA-Binding Proteins Eukaryot. Cell, June 1, 2003; 2(3): 646 - 650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Horino, Y. Sasaki, T. Sasaki, and T. Kenri Multiple Promoter Inversions Generate Surface Antigenic Variation in Mycoplasma penetrans J. Bacteriol., January 1, 2003; 185(1): 231 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Napoli, Y. Zivanovic, C. Bocs, C. Buhler, M. Rossi, P. Forterre, and M. Ciaramella DNA bending, compaction and negative supercoiling by the architectural protein Sso7d of Sulfolobus solfataricus Nucleic Acids Res., June 15, 2002; 30(12): 2656 - 2662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Perez, M. Rehault, and M. Amouyal A functional assay in Escherichia coli to detect non-assisted interaction between galactose repressor dimers Nucleic Acids Res., September 15, 2000; 28(18): 3600 - 3604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Ross and P. Setlow The Bacillus subtilis HBsu Protein Modifies the Effects of alpha /beta -Type, Small Acid-Soluble Spore Proteins on DNA J. Bacteriol., April 1, 2000; 182(7): 1942 - 1948. [Abstract] [Full Text]
|
|
|
|
|
|
A. T. Akhmedov, B. Gross, and R. Jessberger Mammalian SMC3 C-terminal and Coiled-coil Protein Domains Specifically Bind Palindromic DNA, Do Not Block DNA Ends, and Prevent DNA Bending J. Biol. Chem., December 31, 1999; 274(53): 38216 - 38224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Elías-Arnanz and M. Salas Functional interactions between a phage histone-like protein and a transcriptional factor in regulation of phi 29 early-late transcriptional switch Genes & Dev., October 1, 1999; 13(19): 2502 - 2513. [Abstract] [Full Text]
|
|
|
|
|
|
J. Marszalek, W. Zhang, T. R. Hupp, C. Margulies, K. M. Carr, S. Cherry, and J. M. Kaguni Domains of DnaA Protein Involved in Interaction with DnaB Protein, and in Unwinding the Escherichia coli Chromosomal Origin J. Biol. Chem., August 2, 1996; 271(31): 18535 - 18542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Castaing, C. Zelwer, J. Laval, and S. Boiteux HU Protein of Escherichia coli Binds Specifically to DNA That Contains Single-strand Breaks or Gaps J. Biol. Chem., April 28, 1995; 270(17): 10291 - 10296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. T. Paull and R. C. Johnson DNA Looping by Saccharomyces cerevisiae High Mobility Group Proteins NHP6A/B J. Biol. Chem., April 14, 1995; 270(15): 8744 - 8754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Wagner and D. E. Pettijohn Increased DNA-bending Activity and Higher Affinity DNA Binding of High Mobility Group Protein HMG-1 Prepared without Acids J. Biol. Chem., March 31, 1995; 270(13): 7394 - 7398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Toulmé, E. Le Cam, C. Teyssier, E. Delain, P. Sautière, J.-C. Maurizot, and F.ço. Culard Conformational Changes of DNA Minicircles upon the Binding of the Archaebacterial Histone-like Protein MC1 J. Biol. Chem., March 17, 1995; 270(11): 6286 - 6291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Alonso, F. Weise, and F. Rojo The Bacillussubtilis Histone-like Protein Hbsu Is Required for DNA Resolution and DNA Inversion Mediated by the beta Recombinase of Plasmid pSM19035 J. Biol. Chem., February 17, 1995; 270(7): 2938 - 2945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B D Lavoie and G Chaconas Site-specific HU binding in the Mu transpososome: conversion of a sequence-independent DNA-binding protein into a chemical nuclease. Genes & Dev., December 1, 1993; 7(12b): 2510 - 2519. [Abstract] [PDF]
|
|
|
|
|
|
T T Paull, M J Haykinson, and R C Johnson The nonspecific DNA-binding and -bending proteins HMG1 and HMG2 promote the assembly of complex nucleoprotein structures. Genes & Dev., August 1, 1993; 7(8): 1521 - 1534. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
