| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 264, Issue 25, 14797-14805, Sep, 1989
MH Lee and RM Bell
The specificity of the phospholipid cofactor requirement of rat brain
protein kinase C was investigated using Triton X-100 mixed micellar
methods. Sixteen analogues of phosphatidylserine were prepared and tested
for their ability to support protein kinase C activity, [3H]phorbol
12,13-dibutyrate binding, and protein kinase C binding to mixed micelles.
Phosphatidylserinol, -L-serine methyl ester, -N-acetyl- L-serine,
-2-hydroxyacetate, -3-hydroxypropionate, and -4- hydroxybutyrate did not
activate protein kinase C in mixed micelles containing 2 mol % of
sn-1,2-dioleoylglycerol. This indicates that both the carboxyl and amino
moieties are important for activation. Phosphatidyl-D-serine and
-L-homoserine were incapable of supporting full activation; this
demonstrates stereospecificity and the importance of the distance between
the phosphate and carboxyl and amino moieties. Since
1,2-rac-phosphatidyl-L-serine and 1,3-phosphatidyl-L-serine fully supported
protein kinase C activity, the stereochemistry within the glycerol backbone
at the interface was not necessary for maximal activation. Neither
lysophosphatidyl-L-serine nor 1-oleoyl-2-acetyl-sn-
glycero-3-phospho-L-serine supported protein kinase C activity implying
that the interfacial conformation is critical to the activation process.
The phospholipid dependencies of [3H]phorbol 12,13-dibutyrate binding and
of protein kinase C binding to mixed micelles containing sn-
1,2-dioleoylglycerol did not mirror those for activation. The data
demonstrate that protein kinase C possesses a high degree of specificity
with respect to phospholipid activation and implicate several functional
groups within the phospho-L-serine polar head group in binding and
activation.
Phospholipid functional groups involved in protein kinase C activation, phorbol ester binding, and binding to mixed micelles
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. R. Dries and A. C. Newton Kinetic Analysis of the Interaction of the C1 Domain of Protein Kinase C with Lipid Membranes by Stopped-flow Spectroscopy J. Biol. Chem., March 21, 2008; 283(12): 7885 - 7893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wang, J. Silva, K. Krishnamurthy, E. Tran, B. G. Condie, and E. Bieberich Direct Binding to Ceramide Activates Protein Kinase C{zeta} before the Formation of a Pro-apoptotic Complex with PAR-4 in Differentiating Stem Cells J. Biol. Chem., July 15, 2005; 280(28): 26415 - 26424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. S. Lorenzo, J. W. Kung, D. A. Bottorff, S. H. Garfield, J. C. Stone, and P. M. Blumberg Phorbol Esters Modulate the Ras Exchange Factor RasGRP3 Cancer Res., February 1, 2001; 61(3): 943 - 949. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. S. Lorenzo, M. Beheshti, G. R. Pettit, J. C. Stone, and P. M. Blumberg The Guanine Nucleotide Exchange Factor RasGRP Is a High -Affinity Target for Diacylglycerol and Phorbol Esters Mol. Pharmacol., May 1, 2000; 57(5): 840 - 846. [Abstract] [Full Text]
|
|
|
|
|
|
J. W. Lomasney, H.-F. Cheng, S. R. Roffler, and K. King Activation of Phospholipase C delta 1 through C2 Domain by a Ca2+-Enzyme-Phosphatidylserine Ternary Complex J. Biol. Chem., July 30, 1999; 274(31): 21995 - 22001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Nemes, L. N. Marekov, and P. M. Steinert Involucrin Cross-linking by Transglutaminase 1. BINDING TO MEMBRANES DIRECTS RESIDUE SPECIFICITY J. Biol. Chem., April 16, 1999; 274(16): 11013 - 11021. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Sando, O. I. Chertihin, J. M. Owens, and R. H. Kretsinger Contributions to Maxima in Protein Kinase C Activation J. Biol. Chem., December 18, 1998; 273(51): 34022 - 34027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Pepio, X. Fan, and W. S. Sossin The Role of C2 Domains in Ca2+-activated and Ca2+-independent Protein Kinase Cs in Aplysia J. Biol. Chem., July 24, 1998; 273(30): 19040 - 19048. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Johnson, A. S. Edwards, and A. C. Newton A Putative Phosphatidylserine Binding Motif Is Not Involved in the Lipid Regulation of Protein Kinase C J. Biol. Chem., December 5, 1997; 272(49): 30787 - 30792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Lomasney, H.-F. Cheng, L.-P. Wang, Y.-S. Kuan, S.-M. Liu, S. W. Fesik, and K. King Phosphatidylinositol 4,5-Bisphosphate Binding to the Pleckstrin Homology Domain of Phospholipase C-delta 1 Enhances Enzyme Activity J. Biol. Chem., October 11, 1996; 271(41): 25316 - 25326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Munnik, T. d. Vrije, R. F. Irvine, and A. Musgrave Identification of Diacylglycerol Pyrophosphate as a Novel Metabolic Product of Phosphatidic Acid during G-protein Activation in Plants J. Biol. Chem., June 28, 1996; 271(26): 15708 - 15715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ghosh, J. C. Strum, V. A. Sciorra, L. Daniel, and R. M. Bell Raf-1 Kinase Possesses Distinct Binding Domains for Phosphatidylserine and Phosphatidic Acid J. Biol. Chem., April 5, 1996; 271(14): 8472 - 8480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Igarashi, M. Kaneda, A. Yamaji, T. C. Saido, U. Kikkawa, Y. Ono, K. Inoue, and M. Umeda A Novel Phosphatidylserine-binding Peptide Motif Defined by an Anti-idiotypic Monoclonal Antibody J. Biol. Chem., December 8, 1995; 270(49): 29075 - 29078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rytömaa and P. K. J. Kinnunen Reversibility of the Binding of Cytochrome c to Liposomes J. Biol. Chem., February 17, 1995; 270(7): 3197 - 3202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. H. W. Pap, P. A. W. van den Berg, J. W. Borst, and A. J. W. G Visser The Interaction between Protein Kinase C and Lipid Cofactors Studied by Simultaneous Observation of Lipid and Protein Fluorescence J. Biol. Chem., January 20, 1995; 270(3): 1254 - 1260. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
