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J. Biol. Chem., Vol. 264, Issue 34, 20297-20302, 12, 1989
E Padan, N Maisler, D Taglicht, R Karpel and S Schuldiner
We have deleted the chromosomal ant gene from Escherichia coli by
substitution with the kan gene, which encodes kanamycin resistance. The
delta ant strains obtained cannot adapt to high sodium concentrations (700
mM, pH 6.8), which do not affect the wild type. The Na+ sensitivity of
delta ant is pH dependent, increasing at alkaline pH. Thus at pH 8.5, 100
mM NaCl retard growth of delta ant with no effect on the wild type. The
delta ant strains also cannot challenge the toxic effects of Li+ ions, a
substrate of the Na+/H+ antiporter system. However, growth of these strains
is normal on carbon sources which require Na+ ions for transport and
growth. Moreover, antiporter activity, as measured in everted membrane
vesicles, is not significantly impaired. A detailed analysis of the
remaining antiporter activity in a delta ant strain reveals kinetic
properties which differ from those displayed by the ant protein: (a) Km for
transport of Li+ ions is about 15 times higher and (b) the activity is
practically independent of intracellular pH. Our results demonstrate the
presence of an alternative Na+/H+ antiporter(s) in E. coli, additional to
ant system.
Deletion of ant in Escherichia coli reveals its function in adaptation to high salinity and an alternative Na+/H+ antiporter system(s)
Department of Microbial and Molecular Ecology, Life Science Institute, Jerusalem, Israel.
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