| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 264, Issue 36, 21522-21528, 12, 1989
L Fliegel, K Burns, DH MacLennan, RA Reithmeier and M Michalak
A cDNA clone encoding the high affinity Ca2+-binding protein (HACBP) of
rabbit skeletal muscle sarcoplasmic reticulum was isolated and sequenced.
The cDNA encoded a protein of 418 amino acids, but a comparison of the
deduced amino acid sequence with the NH2-terminal amino acid sequence of
the purified protein indicates that a 17-residue NH2-terminal signal
sequence was removed during synthesis. This was confirmed by studies of in
vitro translation of mRNA encoding the protein. Structural predictions did
not reveal any potential transmembrane segments in the protein. The
COOH-terminal sequence of the high affinity Ca2+-binding protein,
Lys-Asp-Glu-Leu, is the same as that proposed to be an endoplasmic
reticulum retention signal (Munro, S., and Pelham, H. R. B. (1987) Cell 48,
899-907). All of these characteristics suggest that the protein is
localized in the lumen of the sarcoplasmic reticulum. The mature protein of
Mr 46,567 contains 109 acidic and 52 basic amino acids. Structural
predictions suggest that the first half of the molecule forms a globular
domain of 8 anti- parallel beta-strands with a helix-turn-helix motif at
the extreme NH2 terminus. The next one-third of the sequence is
proline-rich. This segment can be subdivided into a charged region which
contains a 17- amino acid repeat, followed by a proline, serine, and
threonine-rich segment extending from Pro-246 to Thr-316. Thirty-seven
acidic residues are clustered within 56 amino acids at the COOH terminus of
the protein. Although the protein binds 1 mol of Ca2+/mol with high
affinity, no "EF-hand" consensus sequence was observed in the protein. The
acidic COOH terminus, however, could account for the low affinity, high
capacity Ca2+ binding observed in the protein. In agreement with other
involved laboratories, we have chosen the name calreticulin for the
protein.
Molecular cloning of the high affinity calcium-binding protein (calreticulin) of skeletal muscle sarcoplasmic reticulum
Department of Pediatrics, University of Alberta, Edmonton, Canada.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Nagase, H. Yamakawa, S. Tadokoro, D. Nakajima, S. Inoue, K. Yamaguchi, Y. Itokawa, R. F. Kikuno, H. Koga, and O. Ohara Exploration of Human ORFeome: High-Throughput Preparation of ORF Clones and Efficient Characterization of Their Protein Products DNA Res, June 1, 2008; 15(3): 137 - 149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-Y. Jia, C.-Y. Xu, R.-L. Jing, R.-Z. Li, X.-G. Mao, J.-P. Wang, and X.-P. Chang Molecular cloning and characterization of wheat calreticulin (CRT) gene involved in drought-stressed responses J. Exp. Bot., March 1, 2008; 59(4): 739 - 751. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hageman, M. J. Vos, M. A. W. H. van Waarde, and H. H. Kampinga Comparison of Intra-organellar Chaperone Capacity for Dealing with Stress-induced Protein Unfolding J. Biol. Chem., November 23, 2007; 282(47): 34334 - 34345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. N. Hebert and M. Molinari In and Out of the ER: Protein Folding, Quality Control, Degradation, and Related Human Diseases Physiol Rev, October 1, 2007; 87(4): 1377 - 1408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ottolia, K. D. Philipson, and S. John Xenopus oocyte plasma membrane sheets for FRET analysis Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1519 - C1522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Decca, M. A. Carpio, C. Bosc, M. R. Galiano, D. Job, A. Andrieux, and M. E. Hallak Post-translational Arginylation of Calreticulin: A NEW ISOSPECIES OF CALRETICULIN COMPONENT OF STRESS GRANULES J. Biol. Chem., March 16, 2007; 282(11): 8237 - 8245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-L. Ong, P.-C. Lin, X. Zhang, S.-M. Chia, and H. Yu Kinectin-dependent Assembly of Translation Elongation Factor-1 Complex on Endoplasmic Reticulum Regulates Protein Synthesis J. Biol. Chem., November 3, 2006; 281(44): 33621 - 33634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. B. Williams Beyond lectins: the calnexin/calreticulin chaperone system of the endoplasmic reticulum J. Cell Sci., February 15, 2006; 119(4): 615 - 623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Kolesnikova, B. Berghofer, S. Bamberg, and S. Becker Multivesicular Bodies as a Platform for Formation of the Marburg Virus Envelope J. Virol., November 15, 2004; 78(22): 12277 - 12287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Okamoto, K. Moriishi, T. Miyamura, and Y. Matsuura Intramembrane Proteolysis and Endoplasmic Reticulum Retention of Hepatitis C Virus Core Protein J. Virol., June 15, 2004; 78(12): 6370 - 6380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Ferreira, C. Valck, G. Sanchez, A. Gingras, S. Tzima, M. C. Molina, R. Sim, W. Schwaeble, and A. Ferreira The Classical Activation Pathway of the Human Complement System Is Specifically Inhibited by Calreticulin from Trypanosoma cruzi J. Immunol., March 1, 2004; 172(5): 3042 - 3050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kapoor, H. Srinivas, E. Kandiah, E. Gemma, L. Ellgaard, S. Oscarson, A. Helenius, and A. Surolia Interactions of Substrate with Calreticulin, an Endoplasmic Reticulum Chaperone J. Biol. Chem., February 14, 2003; 278(8): 6194 - 6200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Leach, M. F. Cohen-Doyle, D. Y. Thomas, and D. B. Williams Localization of the Lectin, ERp57 Binding, and Polypeptide Binding Sites of Calnexin and Calreticulin J. Biol. Chem., August 9, 2002; 277(33): 29686 - 29697. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Wedlich-Soldner, I. Schulz, A. Straube, and G. Steinberg Dynein Supports Motility of Endoplasmic Reticulum in the Fungus Ustilago maydis Mol. Biol. Cell, March 1, 2002; 13(3): 965 - 977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-J. Park, D.-G. Lee, J.-R. Yu, S.-k. Jung, K. Choi, J. Lee, J. Lee, Y. S. Kim, J. I. Lee, J. Y. Kwon, et al. Calreticulin, a Calcium-binding Molecular Chaperone, Is Required for Stress Response and Fertility in Caenorhabditis elegans Mol. Biol. Cell, September 1, 2001; 12(9): 2835 - 2845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. L. Roderick, J. D. Lechleiter, and P. Camacho Cytosolic Phosphorylation of Calnexin Controls Intracellular Ca2+ Oscillations via an Interaction with SERCA2b J. Cell Biol., June 12, 2000; 149(6): 1235 - 1248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-S. Yoon, H. Lee, Y. Jung, E. Yu, H.-B. Moon, K. Song, and I. Lee Nuclear Matrix of Calreticulin in Hepatocellular Carcinoma Cancer Res., February 1, 2000; 60(4): 1117 - 1120. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Goto, J. Adjaye, C. H. Rodeck, and M. Monk Identification of genes expressed in human primordial germ cells at the time of entry of the female germ line into meiosis Mol. Hum. Reprod., September 1, 1999; 5(9): 851 - 860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Fadel, E. Dziak, C.-M. Lo, J. Ferrier, N. Mesaeli, M. Michalak, and M. Opas Calreticulin Affects Focal Contact-dependent but Not Close Contact-dependent Cell-substratum Adhesion J. Biol. Chem., May 21, 1999; 274(21): 15085 - 15094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Mesaeli, K. Nakamura, E. Zvaritch, P. Dickie, E. Dziak, K.-H. Krause, M. Opas, D. H. MacLennan, and M. Michalak Calreticulin Is Essential for Cardiac Development J. Cell Biol., March 8, 1999; 144(5): 857 - 868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Knittler, K. Gulow, A. Seelig, and J. C. Howard MHC Class I Molecules Compete in the Endoplasmic Reticulum for Access to Transporter Associated with Antigen Processing J. Immunol., December 1, 1998; 161(11): 5967 - 5977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ohsako, L. Janulis, Y. Hayashi, and D. Bunick Characterization of Domains in Mice of Calnexin-t, a Putative Molecular Chaperone Required in Sperm Fertility, with Use of Glutathione S-Transferase-Fusion Proteins Biol Reprod, November 1, 1998; 59(5): 1214 - 1223. [Abstract] [Full Text]
|
|
|
|
|
|
L. M. John, J. D. Lechleiter, and P. Camacho Differential Modulation of SERCA2 Isoforms by Calreticulin J. Cell Biol., August 24, 1998; 142(4): 963 - 973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Choukhi, S. Ung, C. Wychowski, and J. Dubuisson Involvement of Endoplasmic Reticulum Chaperones in the Folding of Hepatitis C Virus Glycoproteins J. Virol., May 1, 1998; 72(5): 3851 - 3858. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Yabe, T. Nakamura, N. Kanazawa, K. Tashiro, and T. Honjo Calumenin, a Ca2+-binding Protein Retained in the Endoplasmic Reticulum with a Novel Carboxyl-terminal Sequence, HDEF J. Biol. Chem., July 18, 1997; 272(29): 18232 - 18239. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Denning, K. G. Leidal, V. A. Holst, S. S. Iyer, D. W. Pearson, J. R. Clark, W. M. Nauseef, and R. A. Clark Calreticulin Biosynthesis and Processing in Human Myeloid Cells: Demonstration of Signal Peptide Cleavage and N-Glycosylation Blood, July 1, 1997; 90(1): 372 - 381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Michalak, K. Burns, C. Andrin, N. Mesaeli, G. H. Jass, J. L. Busaan, and M. Opas Endoplasmic Reticulum Form of Calreticulin Modulates Glucocorticoid-sensitive Gene Expression J. Biol. Chem., November 15, 1996; 271(46): 29436 - 29445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Mery, N. Mesaeli, M. Michalak, M. Opas, D. P. Lew, and K.-H. Krause Overexpression of Calreticulin Increases Intracellular Ca[IMAGE] Storage and Decreases Store-operated Ca[IMAGE] Influx J. Biol. Chem., April 19, 1996; 271(16): 9332 - 9339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Gray, P. W. Park, T. J. Broekelmann, G. J. Laurent, J. T. Reeves, K. R. Stenmark, and R. P. Mecham The Mitogenic Effects of the Bbeta Chain of Fibrinogen Are Mediated through Cell Surface Calreticulin J. Biol. Chem., November 3, 1995; 270(44): 26602 - 26606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Wada, S.-i. Imai, M. Kai, F. Sakane, and H. Kanoh Chaperone Function of Calreticulin When Expressed in the Endoplasmic Reticulum as the Membrane-anchored and Soluble Forms J. Biol. Chem., September 1, 1995; 270(35): 20298 - 20304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Conway, L. Liu, B. Nowakowski, M. Steiner-Mosonyi, S. P. Ribeiro, and M. Michalak Heat Shock-sensitive Expression of Calreticulin J. Biol. Chem., July 14, 1995; 270(28): 17011 - 17016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kuwabara, D. J. Pinsky, A. M. Schmidt, C. Benedict, J. Brett, S. Ogawa, M. J. Broekman, A. J. Marcus, R. R. Sciacca, M. Michalak, et al. Calreticulin, an Antithrombotic Agent Which Binds to Vitamin K-dependent Coagulation Factors, Stimulates Endothelial Nitric Oxide Production, and Limits Thrombosis in Canine Coronary Arteries J. Biol. Chem., April 7, 1995; 270(14): 8179 - 8187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Goicoechea, A. W. Orr, M. A. Pallero, P. Eggleton, and J. E. Murphy-Ullrich Thrombospondin Mediates Focal Adhesion Disassembly through Interactions with Cell Surface Calreticulin J. Biol. Chem., November 10, 2000; 275(46): 36358 - 36368. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
