HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bligny, R. Articles by Douce, R. Search for Related Content PubMed PubMed Citation Articles by Bligny, R. Articles by Douce, R. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 264, Issue 9, 4888-4895, 03, 1989

Transport and phosphorylation of choline in higher plant cells. Phosphorus-31 nuclear magnetic resonance studies

R Bligny, MF Foray, C Roby and R Douce
Laboratoire de Physiologie Cellulaire Vegetale, Centre d'Etudes Nucleaires, Grenoble, France.

When sycamore cells were suspended in basal medium containing choline, the latter was taken up by the cells very rapidly. A facilitated diffusion system appertained at low concentrations of choline and exhibited Michaelis-Menten kinetics. At higher choline concentrations simple diffusion appeared to be the principal mode of uptake. Addition of choline to the perfusate of compressed sycamore cells monitored by 31P NMR spectroscopy resulted in a dramatic accumulation of P-choline in the cytoplasmic compartment containing choline kinase and not in the vacuole. The total accumulation of P-choline over a 10-h period exhibited Michaelis-Menten kinetics. During this period, in the absence of Pi in the perfusion medium there was a marked depletion of glucose-6- P, and the cytoplasmic Pi resonance disappeared almost completely. When a threshold of cytoplasmic Pi was attained, the phosphorylation of choline was sustained by the continuous release of Pi from the vacuole although at a much lower rate. However, when 100 microM inorganic phosphate was present in the perfusion medium, externally added Pi was preferentially used to sustain P-choline synthesis. It is clear, therefore, that cytosolic choline kinase associated with a carrier- mediated transport system for choline uptake appeared as effective systems for continuously trapping cytoplasmic Pi including vacuolar Pi entering the cytoplasm.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. Inoue and Y. Moriyasu Autophagy is not a Main Contributor to the Degradation of Phospholipids in Tobacco Cells Cultured under Sucrose Starvation Conditions Plant Cell Physiol., April 1, 2006; 47(4): 471 - 480. [Abstract] [Full Text] [PDF]

				S. Nogues, G. Tcherkez, G. Cornic, and J. Ghashghaie Respiratory Carbon Metabolism following Illumination in Intact French Bean Leaves Using 13C/12C Isotope Labeling Plant Physiology, October 1, 2004; 136(2): 3245 - 3254. [Abstract] [Full Text] [PDF]

				B. van der Rest, A.-M. Boisson, E. Gout, R. Bligny, and R. Douce Glycerophosphocholine Metabolism in Higher Plant Cells. Evidence of a New Glyceryl-Phosphodiester Phosphodiesterase Plant Physiology, September 1, 2002; 130(1): 244 - 255. [Abstract] [Full Text] [PDF]

				S. D. McNeil, M. L. Nuccio, M. J. Ziemak, and A. D. Hanson Enhanced synthesis of choline and glycine betaine in transgenic tobacco plants that overexpress phosphoethanolamine N-methyltransferase PNAS, July 24, 2001; (2001) 171228998. [Abstract] [Full Text] [PDF]

				S. D. McNeil, D. Rhodes, B. L. Russell, M. L. Nuccio, Y. Shachar-Hill, and A. D. Hanson Metabolic Modeling Identifies Key Constraints on an Engineered Glycine Betaine Synthesis Pathway in Tobacco Plant Physiology, September 1, 2000; 124(1): 153 - 162. [Abstract] [Full Text]

				S. D. McNeil, M. L. Nuccio, M. J. Ziemak, and A. D. Hanson Enhanced synthesis of choline and glycine betaine in transgenic tobacco plants that overexpress phosphoethanolamine N-methyltransferase PNAS, August 14, 2001; 98(17): 10001 - 10005. [Abstract] [Full Text] [PDF]