|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 265, Issue 11, 5938-5941, Apr, 1990
SC Bodary and JW McLean
We describe a novel integrin heterodimer on the surface of the human
embryonic kidney cell line 293. This receptor is comprised of alpha v and
beta 1 subunits, each of which has been previously found in association
with other integrin subunits. This alpha v.beta 1 complex was identified as
the predominant vitronectin receptor (VnR) on the surface of 293 cells by
immunoprecipitation with antibodies raised against the alpha v subunit.
Polymerase chain reaction analysis detected mRNAs for alpha v and beta 1
subunits while no evidence was obtained for beta 2, beta 3, or alpha IIb
integrin subunit mRNA. Immunoprecipitation of surface-iodinated proteins
with antibodies to alpha v gave bands of 150 and 120 kDa. The 120-kDa band
reacted with antibodies to beta 1 in immunoblotting experiments. 293 cells
adhere to vitronectin, fibronectin, laminin, and collagen IV, while von
Willebrand factor and fibrinogen, known ligands of the VnR (alpha v.beta
3), did not support adhesion. A polyclonal antibody directed against both
subunits of the VnR (alpha v, beta 3) inhibits attachment of 293 cells to
vitronectin but not to other adhesive proteins. A beta 1-specific
monoclonal inhibited attachment to fibronectin, laminin, and collagen IV,
known ligands of beta 1 integrins, as well as vitronectin. This novel
(alpha v. beta 1) VnR thus appears to mediate cell adhesion exclusively to
vitronectin, in contrast to previously described VnRs which have multiple
ligands.
The integrin beta 1 subunit associates with the vitronectin receptor alpha v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line
Department of Biomolecular Chemistry, Genentech, Inc., South San Francisco, California 94080.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  V. Gupta, J. L. Alonso, T. Sugimori, M. Issafi, J.-P. Xiong, and M. A. Arnaout Role of the {beta}-Subunit Arginine/Lysine Finger in Integrin Heterodimer Formation and Function J. Immunol., February 1, 2008; 180(3): 1713 - 1718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Soundararajan, B. W. Lindsey, C. Leopold, and V. F. Rafuse Easy and Rapid Differentiation of Embryonic Stem Cells into Functional Motoneurons Using Sonic Hedgehog-Producing Cells Stem Cells, July 1, 2007; 25(7): 1697 - 1706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Larson, D. C. Brown, C. Ye, and B. Hjelle Peptide Antagonists That Inhibit Sin Nombre Virus and Hantaan Virus Entry through the {beta}3-Integrin Receptor J. Virol., June 15, 2005; 79(12): 7319 - 7326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kedzierski, J. Montgomery, D. Bullen, J. Curtis, E. Gardiner, A. Jimenez-Ruiz, and E. Handman A Leucine-Rich Repeat Motif of Leishmania Parasite Surface Antigen 2 Binds to Macrophages through the Complement Receptor 3 J. Immunol., April 15, 2004; 172(8): 4902 - 4906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Matsumoto-Miyai, A. Ninomiya, H. Yamasaki, H. Tamura, Y. Nakamura, and S. Shiosaka NMDA-Dependent Proteolysis of Presynaptic Adhesion Molecule L1 in the Hippocampus by Neuropsin J. Neurosci., August 27, 2003; 23(21): 7727 - 7736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Markotic, L. Hensley, T. Geisbert, K. Spik, and C. Schmaljohn Hantaviruses induce cytopathic effects and apoptosis in continuous human embryonic kidney cells J. Gen. Virol., August 1, 2003; 84(8): 2197 - 2202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Buhusi, B. R. Midkiff, A. M. Gates, M. Richter, M. Schachner, and P. F. Maness Close Homolog of L1 Is an Enhancer of Integrin-mediated Cell Migration J. Biol. Chem., June 27, 2003; 278(27): 25024 - 25031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Hotchkiss, A. W. Ashton, and E. L. Schwartz Thymidine Phosphorylase and 2-Deoxyribose Stimulate Human Endothelial Cell Migration by Specific Activation of the Integrins {alpha}5{beta}1 and {alpha}V{beta}3 J. Biol. Chem., May 23, 2003; 278(21): 19272 - 19279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-J. Wang, J.-C. Kuo, C.-C. Yao, and R.-H. Chen DAP-kinase induces apoptosis by suppressing integrin activity and disrupting matrix survival signals J. Cell Biol., October 14, 2002; 159(1): 169 - 179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Koistinen and J. Heino The Selective Regulation of alpha Vbeta 1 Integrin Expression Is Based on the Hierarchical Formation of alpha V-containing Heterodimers J. Biol. Chem., June 28, 2002; 277(27): 24835 - 24841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Thelen, V. Kedar, A. K. Panicker, R.-S. Schmid, B. R. Midkiff, and P. F. Maness The Neural Cell Adhesion Molecule L1 Potentiates Integrin-Dependent Cell Migration to Extracellular Matrix Proteins J. Neurosci., June 15, 2002; 22(12): 4918 - 4931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Jackson, A. P. Mould, D. Sheppard, and A. M. Q. King Integrin {alpha}v{beta}1 Is a Receptor for Foot-and-Mouth Disease Virus J. Virol., February 1, 2002; 76(3): 935 - 941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tadokoro, Y. Tomiyama, S. Honda, H. Kashiwagi, S. Kosugi, M. Shiraga, T. Kiyoi, Y. Kurata, and Y. Matsuzawa Missense mutations in the beta 3 subunit have a different impact on the expression and function between alpha IIbbeta 3 and alpha vbeta 3 Blood, February 1, 2002; 99(3): 931 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Li, S. L. Brown, D. G. Stupack, X. S. Puente, D. A. Cheresh, and G. R. Nemerow Integrin {alpha}v{beta}1 Is an Adenovirus Coreceptor J. Virol., June 1, 2001; 75(11): 5405 - 5409. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. J. Yarwood and J. R. Woodgett Extracellular matrix composition determines the transcriptional response to epidermal growth factor receptor activation PNAS, March 29, 2001; (2001) 81069098. [Abstract] [Full Text]
|
|
|
|
|
|
S. Honda, Y. Tomiyama, N. Pampori, H. Kashiwagi, T. Kiyoi, S. Kosugi, S. Tadokoro, Y. Kurata, S. J. Shattil, and Y. Matsuzawa Ligand binding to integrin {alpha}v{beta}3 requires tyrosine 178 in the {alpha}v subunit Blood, January 1, 2001; 97(1): 175 - 182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-S. Li, L. Zhang, J. Gu, N. D. Amin, and H. C. Pant Integrin alpha 1beta 1-Mediated Activation of Cyclin-Dependent Kinase 5 Activity Is Involved in Neurite Outgrowth and Human Neurofilament Protein H Lys-Ser-Pro Tail Domain Phosphorylation J. Neurosci., August 15, 2000; 20(16): 6055 - 6062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Nichols, T. d. Laney, B. Zheng, D. A. Bellinger, G. A. Nickols, W. Engleman, and D. R. Clemmons Reduction in Atherosclerotic Lesion Size in Pigs by {alpha}V{beta}3 Inhibitors Is Associated With Inhibition of Insulin-Like Growth Factor-I-Mediated Signaling Circ. Res., November 26, 1999; 85(11): 1040 - 1045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Nakamura, H. Tanaka, G. A. Rodan, and L. T. Duong Echistatin Inhibits the Migration of Murine Prefusion Osteoclasts and the Formation of Multinucleated Osteoclast-Like Cells Endocrinology, December 1, 1998; 139(12): 5182 - 5193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Vitale, T. Di Matola, G. Fenzi, M. Illario, and G. Rossi Fibronectin Is Required to Prevent Thyroid Cell Apoptosis through an Integrin-Mediated Adhesion Mechanism J. Clin. Endocrinol. Metab., October 1, 1998; 83(10): 3673 - 3680. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. S. Munger, J. G. Harpel, F. G. Giancotti, and D. B. Rifkin Interactions between Growth Factors and Integrins: Latent Forms of Transforming Growth Factor-beta Are Ligands for the Integrin alpha vbeta 1 Mol. Biol. Cell, September 1, 1998; 9(9): 2627 - 2638. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. Xie, G.F. Clunn, J.S. Lymn, and A.D. Hughes Role of intracellular calcium ([Ca2+]i) and tyrosine phosphorylation in adhesion of cultured vascular smooth muscle cells to fibrinogen Cardiovasc Res, August 1, 1998; 39(2): 475 - 484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. E. Slack Tyrosine phosphorylation of paxillin and focal adhesion kinase by activation of muscarinic m3 receptors is dependent on integrin engagement by the extracellular matrix PNAS, June 23, 1998; 95(13): 7281 - 7286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Coburn, L. Magoun, S. C. Bodary, and J. M. Leong Integrins alpha vbeta 3 and alpha 5beta 1 Mediate Attachment of Lyme Disease Spirochetes to Human Cells Infect. Immun., May 1, 1998; 66(5): 1946 - 1952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Wilhide, Y. Jin, Q. Guo, L. Li, S.-X. Li, E. Rubin, and P. F. Bray The Human Integrin beta 3 Gene Is 63 kb and Contains a 5'-UTR Sequence Regulating Expression Blood, November 15, 1997; 90(10): 3951 - 3961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. O. Simon, E. M. Nutt, D. G. Abraham, G. A. Rodan, and L. T. Duong The alpha vbeta 3 Integrin Regulates alpha 5beta 1-mediated Cell Migration toward Fibronectin J. Biol. Chem., November 14, 1997; 272(46): 29380 - 29389. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Kumar, H.-N. C. P. Rogers, M. Malkowski, E. Maxwell, J. J. Catino, and L. Armstrong Biochemical Characterization of the Binding of Echistatin to Integrin alpha vbeta 3 Receptor J. Pharmacol. Exp. Ther., November 1, 1997; 283(2): 843 - 853. [Abstract] [Full Text]
|
|
|
|
|
|
R. Milner, G. Edwards, C. Streuli, and C. ffrench-Constant A Role in Migration for the alpha vbeta 1 Integrin Expressed on Oligodendrocyte Precursors J. Neurosci., November 15, 1996; 16(22): 7240 - 7252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Echtermeyer, S. Schöber, E. Pöschl, H. von der Mark, and K. von der Mark Specific Induction of Cell Motility on Laminin by alpha7 Integrin J. Biol. Chem., January 26, 1996; 271(4): 2071 - 2075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Hu, E. C. K. Lin, N. L. Kovach, J. R. Hoyer, and J. W. Smith A Biochemical Characterization of the Binding of Osteopontin to Integrins alpha(v)beta(1) and alpha(v)beta(5) J. Biol. Chem., November 3, 1995; 270(44): 26232 - 26238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Schnapp, N. Hatch, D. M. Ramos, I. V. Klimanskaya, D. Sheppard, and R. Pytela The Human Integrin alpha8beta1 Functions as a Receptor for Tenascin, Fibronectin, and Vitronectin J. Biol. Chem., September 29, 1995; 270(39): 23196 - 23202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bhattacharya, C. Fu, J. Bhattacharya, and S. Greenberg Soluble Ligands of the [IMAGE][IMAGE][IMAGE][IMAGE] Integrin Mediate Enhanced Tyrosine Phosphorylation of Multiple Proteins in Adherent Bovine Pulmonary Artery Endothelial Cells J. Biol. Chem., July 14, 1995; 270(28): 16781 - 16787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Katagiri, T. Hiroyama, N. Akamatsu, H. Suzuki, H. Yamazaki, and K. Tanoue Involvement of alpha(v)beta(3) Integrin in Mediating Fibrin Gel Retraction J. Biol. Chem., January 27, 1995; 270(4): 1785 - 1790. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gladson, J. Wilcox, L Sanders, G. Gillespie, and D. Cheresh Cerebral microenvironment influences expression of the vitronectin gene in astrocytic tumors J. Cell Sci., January 3, 1995; 108(3): 947 - 956. [Abstract] [PDF]
|
|
|
|
|
|
J. Marshall, D. Rutherford, A. McCartney, F Mitjans, S. Goodman, and I. Hart Alpha v beta 1 is a receptor for vitronectin and fibrinogen, and acts with alpha 5 beta 1 to mediate spreading on fibronectin J. Cell Sci., January 3, 1995; 108(3): 1227 - 1238. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Delannet, F. Martin, B. Bossy, D. A. Cheresh, L. F. Reichardt, and J. L. Duband Specific roles of the alpha V beta 1, alpha V beta 3 and alpha V beta 5 integrins in avian neural crest cell adhesion and migration on vitronectin Development, September 1, 1994; 120(9): 2687 - 2702. [Abstract] [PDF]
|
|
|
|
|
|
R Milner and C Ffrench-Constant A developmental analysis of oligodendroglial integrins in primary cells: changes in alpha v-associated beta subunits during differentiation Development, January 12, 1994; 120(12): 3497 - 3506. [Abstract] [PDF]
|
|
|
|
|
|
J. T. Yang, H. Rayburn, and R. O. Hynes Embryonic mesodermal defects in alpha 5 integrin-deficient mice Development, December 1, 1993; 119(4): 1093 - 1105. [Abstract] [PDF]
|
|
|
|
|
|
G Zambruno, P. Marchisio, A Melchiori, S Bondanza, R Cancedda, and M De Luca Expression of integrin receptors and their role in adhesion, spreading and migration of normal human melanocytes J. Cell Sci., January 5, 1993; 105(1): 179 - 190. [Abstract] [PDF]
|
|
|
|
 |
|
 V.-J. Uitto and H. Larjava Extracellular Matrix Molecules and their Receptors: An Overview with Special Emphasis on Periodontal Tissues Critical Reviews in Oral Biology & Medicine, January 1, 1991; 2(3): 323 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Spizz and P. J. Blackshear Overexpression of the Myristoylated Alanine-rich C-kinase Substrate Inhibits Cell Adhesion to Extracellular Matrix Components J. Biol. Chem., August 17, 2001; 276(34): 32264 - 32273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Yarwood and J. R. Woodgett Extracellular matrix composition determines the transcriptional response to epidermal growth factor receptor activation PNAS, April 10, 2001; 98(8): 4472 - 4477. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |