J. Biol. Chem., Vol. 265, Issue 11, 5956-5959, Apr, 1990
Release of dialkylglycerol from purple membrane phospholipids by phospholipase D
A Muga, JL Arrondo, JI Gurtubay and FM Goni
Department of Biochemistry, University of Basque Country, Bilbao, Spain.
When the major polar lipid of purple membrane, a dialkyl analogue of
phosphatidyl glycerophosphate, is treated with phospholipase D under the
usual assay conditions for this enzyme, the reaction yields dialkylglycerol
and glycerol bisphosphate, i.e. the kind of products that would be expected
from a phospholipase C reaction. The effect is seen both in native purple
membranes and with the pure phospholipid in the form of liposomes. The
specific activity and kinetic parameters Km and Vmax of phospholipase D for
the purple membrane phospholipid are similar to those for egg
phosphatidylcholine. The presence of phospholipase C impurities in the
phospholipase D preparations has been ruled out as an explanation for the
above observations. A hypothesis is suggested, taking into account the
peculiar headgroup structure of the bacterial lipid, to explain the
seemingly anomalous enzyme behavior.
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