HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hirata, R. Articles by Anraku, Y. Search for Related Content PubMed PubMed Citation Articles by Hirata, R. Articles by Anraku, Y. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 265, Issue 12, 6726-6733, Apr, 1990

Molecular structure of a gene, VMA1, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae

R Hirata, Y Ohsumk, A Nakano, H Kawasaki, K Suzuki and Y Anraku
Department of Biology, Faculty of Science, University of Tokyo, Japan.

Subunit a of the vacuolar membrane H(+)-translocating adenosine triphosphatase of the yeast Saccharomyces cerevisiae contains a catalytic site for ATP hydrolysis. N-terminal sequences of six tryptic peptides of the subunit were determined. Based on the peptide sequence information, a 39-base oligonucleotide probe was synthesized, and the gene encoding the subunit (VMA1) was isolated from a genomic DNA library by hybridization. The nucleotide sequence of the gene predicts a polypeptide of 1,071 amino acids with a calculated molecular mass of 118,635 daltons, which is much larger than the value 67 kDa estimated on sodium dodecyl sulfate-polyacrylamide gels. N- and C-terminal regions of the deduced sequence (residues 1-284 and 739-1,071) are very similar to those of the catalytic subunits of carrot (69 kDa) and Neurospora crassa (67 kDa) vacuolar membrane H(+)-ATPases (62 and 73% identity over 600 residues, respectively). The homologous regions also show about 25% sequence identity over 400 residues with beta-subunits of F0F1-ATPases. In contrast, the internal region containing 454 amino acid residues (residues 285-738) shows no detectable sequence similarities to any known ATPase subunits and instead is similar to a yeast endonuclease encoded by the HO gene. None of the six tryptic peptides is located in this internal region. Northern blotting analysis detected a single mRNA of 3.5 kilobases, indicating that the gene has no introns. Although the reason for the discrepancy in molecular mass is unclear at present, these results suggest that a novel processing mechanism, which might involve a post-translational excision of the internal region followed by peptide ligation, operates on the yeast VMA1 product. The VMA1 gene has proven to be the same gene as the TFP1 gene (Shih, C.-K., Wagner, R., Feinstein, S., Kanik-Ennulat, C., and Neff, N. (1988) Mol. Cell. Biol. 8, 3094-3103) whose dominant mutant allele (TFP1-408) confers a dominant trifluoperazine resistance and Ca2(+)-sensitive growth. This and our findings suggest that the vacuolar membrane H(+)-ATPase participates in maintenance of cytoplasmic Ca2+ homeostasis.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. Bakhrat, K. Baranes, O. Krichevsky, I. Rom, G. Schlenstedt, S. Pietrokovski, and D. Raveh Nuclear Import of Ho Endonuclease Utilizes Two Nuclear Localization Signals and Four Importins of the Ribosomal Import System J. Biol. Chem., May 5, 2006; 281(18): 12218 - 12226. [Abstract] [Full Text] [PDF]

				M. Shimazu, T. Sekito, K. Akiyama, Y. Ohsumi, and Y. Kakinuma A Family of Basic Amino Acid Transporters of the Vacuolar Membrane from Saccharomyces cerevisiae J. Biol. Chem., February 11, 2005; 280(6): 4851 - 4857. [Abstract] [Full Text] [PDF]

				E. Shao and M. Forgac Involvement of the Nonhomologous Region of Subunit A of the Yeast V-ATPase in Coupling and in Vivo Dissociation J. Biol. Chem., November 19, 2004; 279(47): 48663 - 48670. [Abstract] [Full Text] [PDF]

				A. R. Flannery, L. A. Graham, and T. H. Stevens Topological Characterization of the c, c', and c'' Subunits of the Vacuolar ATPase from the Yeast Saccharomyces cerevisiae J. Biol. Chem., September 17, 2004; 279(38): 39856 - 39862. [Abstract] [Full Text] [PDF]

				K. L. Posey, V. Koufopanou, A. Burt, and F. S. Gimble Evolution of divergent DNA recognition specificities in VDE homing endonucleases from two yeast species Nucleic Acids Res., July 27, 2004; 32(13): 3947 - 3956. [Abstract] [Full Text] [PDF]

				A. Romanelli, A. Shekhtman, D. Cowburn, and T. W. Muir Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction PNAS, April 27, 2004; 101(17): 6397 - 6402. [Abstract] [Full Text] [PDF]

				G. Butler, C. Kenny, A. Fagan, C. Kurischko, C. Gaillardin, and K. H. Wolfe Evolution of the MAT locus and its Ho endonuclease in yeast species PNAS, February 10, 2004; 101(6): 1632 - 1637. [Abstract] [Full Text] [PDF]

				A. Bakhrat, M. S. Jurica, B. L. Stoddard, and D. Raveh Homology Modeling and Mutational Analysis of Ho Endonuclease of Yeast Genetics, February 1, 2004; 166(2): 721 - 728. [Abstract] [Full Text] [PDF]

				T. Miyake, H. Hiraishi, H. Sammoto, and B.-I. Ono Involvement of the VDE Homing Endonuclease and Rapamycin in Regulation of the Saccharomyces cerevisiae GSH11 Gene Encoding the High Affinity Glutathione Transporter J. Biol. Chem., October 10, 2003; 278(41): 39632 - 39636. [Abstract] [Full Text] [PDF]

				Y. Ding, M.-Q. Xu, I. Ghosh, X. Chen, S. Ferrandon, G. Lesage, and Z. Rao Crystal Structure of a Mini-intein Reveals a Conserved Catalytic Module Involved in Side Chain Cyclization of Asparagine during Protein Splicing J. Biol. Chem., October 3, 2003; 278(40): 39133 - 39142. [Abstract] [Full Text] [PDF]

				E. Shao, T. Nishi, S. Kawasaki-Nishi, and M. Forgac Mutational Analysis of the Non-homologous Region of Subunit A of the Yeast V-ATPase J. Biol. Chem., April 4, 2003; 278(15): 12985 - 12991. [Abstract] [Full Text] [PDF]

				Y. Nagai, S. Nogami, F. Kumagai-Sano, and Y. Ohya Karyopherin-Mediated Nuclear Import of the Homing Endonuclease VMA1-Derived Endonuclease Is Required for Self-Propagation of the Coding Region Mol. Cell. Biol., March 1, 2003; 23(5): 1726 - 1736. [Abstract] [Full Text] [PDF]

				I. Saves, C. Morlot, L. Thion, J.-L. Rolland, J. Dietrich, and J.-M. Masson Investigating the endonuclease activity of four Pyrococcus abyssi inteins Nucleic Acids Res., October 1, 2002; 30(19): 4158 - 4165. [Abstract] [Full Text] [PDF]

				I. Saves, H. Eleaume, J. Dietrich, and J.-M. Masson The Thy Pol-2 intein of Thermococcus hydrothermalis is an isoschizomer of PI-TliI and PI-TfuII endonucleases Nucleic Acids Res., November 1, 2000; 28(21): 4391 - 4396. [Abstract] [Full Text] [PDF]

				K. Frischkorn, B. Springer, E. C. Böttger, E. O. Davis, M. J. Colston, and P. Sander In Vivo Splicing and Functional Characterization of Mycobacterium leprae RecA J. Bacteriol., June 15, 2000; 182(12): 3590 - 3592. [Abstract] [Full Text]

				C. Saguez, G. Lecellier, and F. Koll Intronic GIY-YIG endonuclease gene in the mitochondrial genome of Podospora curvicolla: evidence for mobility Nucleic Acids Res., March 15, 2000; 28(6): 1299 - 1306. [Abstract] [Full Text] [PDF]

				I. Saves, V. Ozanne, J. Dietrich, and J.-M. Masson Inteins of Thermococcus fumicolans DNA Polymerase Are Endonucleases with Distinct Enzymatic Behaviors J. Biol. Chem., January 28, 2000; 275(4): 2335 - 2341. [Abstract] [Full Text] [PDF]

				D. Hu, M. Crist, X. Duan, F. A. Quiocho, and F. S. Gimble Probing the Structure of the PI-SceI-DNA Complex by Affinity Cleavage and Affinity Photocross-linking J. Biol. Chem., January 28, 2000; 275(4): 2705 - 2712. [Abstract] [Full Text] [PDF]

				E. Vasilyeva, Q. Liu, K. J. MacLeod, J. D. Baleja, and M. Forgac Cysteine Scanning Mutagenesis of the Noncatalytic Nucleotide Binding Site of the Yeast V-ATPase J. Biol. Chem., January 7, 2000; 275(1): 255 - 260. [Abstract] [Full Text] [PDF]

				K. J. MacLeod, E. Vasilyeva, K. Merdek, P. D. Vogel, and M. Forgac Photoaffinity Labeling of Wild-type and Mutant Forms of the Yeast V-ATPase A Subunit by 2-Azido-[32P]ADP J. Biol. Chem., November 12, 1999; 274(46): 32869 - 32874. [Abstract] [Full Text] [PDF]

				C. Landolt-Marticorena, W. H. Kahr, P. Zawarinski, J. Correa, and M. F. Manolson Substrate- and Inhibitor-induced Conformational Changes in the Yeast V-ATPase Provide Evidence for Communication between the Catalytic and Proton-translocating Sectors J. Biol. Chem., September 10, 1999; 274(37): 26057 - 26064. [Abstract] [Full Text] [PDF]

				C. Forster, M. A. Santos, S. Ruffert, R. Kramer, and J. L. Revuelta Physiological Consequence of Disruption of the VMA1 Gene in the Riboflavin Overproducer Ashbya gossypii J. Biol. Chem., April 2, 1999; 274(14): 9442 - 9448. [Abstract] [Full Text] [PDF]

				N. Nelson and W. R. Harvey Vacuolar and Plasma Membrane Proton-Adenosinetriphosphatases Physiol Rev, April 1, 1999; 79(2): 361 - 385. [Abstract] [Full Text] [PDF]

				J. E. G. McCarthy Posttranscriptional Control of Gene Expression in Yeast Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1492 - 1553. [Abstract] [Full Text] [PDF]

				R. Ros, C. Montesinos, A. Rimon, E. Padan, and R. Serrano J. Bacteriol., June 15, 1998; 180(12): 3131 - 3136. [Abstract] [Full Text]

				K. Nishimura, K. Igarashi, and Y. Kakinuma Proton Gradient-Driven Nickel Uptake by Vacuolar Membrane Vesicles of Saccharomyces cerevisiae J. Bacteriol., April 1, 1998; 180(7): 1962 - 1964. [Abstract] [Full Text]

				V. Lazarevic, B. Soldo, A. Dusterhoft, H. Hilbert, C. Mauel, and D. Karamata Introns and intein coding sequence in the ribonucleotide reductase genes of Bacillus subtilis temperate bacteriophage SPbeta PNAS, February 17, 1998; 95(4): 1692 - 1697. [Abstract] [Full Text] [PDF]

				K. J. MacLeod, E. Vasilyeva, J. D. Baleja, and M. Forgac Mutational Analysis of the Nucleotide Binding Sites of the Yeast Vacuolar Proton-translocating ATPase J. Biol. Chem., January 2, 1998; 273(1): 150 - 156. [Abstract] [Full Text] [PDF]

				D. D. Jackson and T. H. Stevens VMA12 Encodes a Yeast Endoplasmic Reticulum Protein Required for Vacuolar H+-ATPase Assembly J. Biol. Chem., October 10, 1997; 272(41): 25928 - 25934. [Abstract] [Full Text] [PDF]

				M. Kawasaki, S. Nogami, Y. Satow, Y. Ohya, and Y. Anraku Identification of Three Core Regions Essential for Protein Splicing of the Yeast Vma1 Protozyme. A RANDOM MUTAGENESIS STUDY OF THE ENTIRE VMA1-DERIVED ENDONUCLEASE SEQUENCE J. Biol. Chem., June 20, 1997; 272(25): 15668 - 15674. [Abstract] [Full Text] [PDF]

				Q. Liu, X.-H. Leng, P. R. Newman, E. Vasilyeva, P. M. Kane, and M. Forgac Site-directed Mutagenesis of the Yeast V-ATPase A Subunit J. Biol. Chem., May 2, 1997; 272(18): 11750 - 11756. [Abstract] [Full Text] [PDF]

				R. Hirata, L. A. Graham, A. Takatsuki, T. H. Stevens, and Y. Anraku VMA11 and VMA16 Encode Second and Third Proteolipid Subunits of the Saccharomyces cerevisiae Vacuolar Membrane H+-ATPase J. Biol. Chem., February 21, 1997; 272(8): 4795 - 4803. [Abstract] [Full Text] [PDF]

				X.-H. Leng, M. F. Manolson, Q. Liu, and M. Forgac Site-directed Mutagenesis of the 100-kDa Subunit (Vph1p) of the Yeast Vacuolar (H+)-ATPase J. Biol. Chem., September 13, 1996; 271(37): 22487 - 22493. [Abstract] [Full Text] [PDF]

				R. Wilms, C. Freiberg, E. Wegerle, I. Meier, F. Mayer, and V. Muller Subunit Structure and Organization of the Genes of the A1A0 ATPase from the Archaeon Methanosarcina mazei Go1 J. Biol. Chem., August 2, 1996; 271(31): 18843 - 18852. [Abstract] [Full Text] [PDF]

				E. Vasilyeva and M. Forgac 3'-O-(4-Benzoyl)benzoyladenosine 5'-Triphosphate Inhibits Activity of the Vacuolar (H+)-ATPase from Bovine Brain Clathrin-coated Vesicles by Modification of a Rapidly Exchangeable, Noncatalytic Nucleotide Binding Site on the B Subunit J. Biol. Chem., May 31, 1996; 271(22): 12775 - 12782. [Abstract] [Full Text] [PDF]

				J. J. Tomashek, J. L. Sonnenburg, J. M. Artimovich, and D. J. Klionsky Resolution of Subunit Interactions and Cytoplasmic Subcomplexes of the Yeast Vacuolar Proton-translocating ATPase J. Biol. Chem., April 26, 1996; 271(17): 10397 - 10404. [Abstract] [Full Text] [PDF]

				Q. Liu, P. M. Kane, P. R. Newman, and M. Forgac Site-directed Mutagenesis of the Yeast V-ATPase B Subunit (Vma2p) J. Biol. Chem., January 26, 1996; 271(4): 2018 - 2022. [Abstract] [Full Text] [PDF]

				Y Xie, M. Coukell, and Z Gombos Antisense RNA inhibition of the putative vacuolar H(+)-ATPase proteolipid of Dictyostelium reduces intracellular Ca2+ transport and cell viability J. Cell Sci., January 2, 1996; 109(2): 489 - 497. [Abstract] [PDF]

				K. J. Hill and T. H. Stevens Vma22p Is a Novel Endoplasmic Reticulum-associated Protein Required for Assembly of the Yeast Vacuolar H[IMAGE]-ATPase Complex J. Biol. Chem., September 22, 1995; 270(38): 22329 - 22336. [Abstract] [Full Text] [PDF]

				J. Zhang, E. Vasilyeva, Y. Feng, and M. Forgac J. Biol. Chem., June 30, 1995; 270(26): 15494 - 15500. [Abstract] [Full Text] [PDF]

				L. A. Graham, K. J. Hill, and T. H. Stevens VMA8 Encodes a 32-kDa V[IMAGE] Subunit of the Saccharomyces cerevisiae Vacuolar H[IMAGE]-ATPase Required for Function and Assembly of the Enzyme Complex J. Biol. Chem., June 23, 1995; 270(25): 15037 - 15044. [Abstract] [Full Text] [PDF]

				I. Tanida, A. Hasegawa, H. Iida, Y. Ohya, and Y. Anraku Cooperation of Calcineurin and Vacuolar H[IMAGE]-ATPase in Intracellular Ca[IMAGE]Homeostasis of Yeast Cells J. Biol. Chem., April 28, 1995; 270(17): 10113 - 10119. [Abstract] [Full Text] [PDF]

				F. S. Gimble and B. W. Stephens Substitutions in Conserved Dodecapeptide Motifs That Uncouple the DNA Binding and DNA Cleavage Activities of PI-SceI Endonuclease J. Biol. Chem., March 17, 1995; 270(11): 5849 - 5856. [Abstract] [Full Text] [PDF]

				P. Kane, C. Yamashiro, D. Wolczyk, N Neff, M Goebl, and T. Stevens Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase Science, November 2, 1990; 250(4981): 651 - 657. [Abstract] [PDF]

				T. Xu and M. Forgac Subunit D (Vma8p) of the Yeast Vacuolar H+-ATPase Plays a Role in Coupling of Proton Transport and ATP Hydrolysis J. Biol. Chem., July 14, 2000; 275(29): 22075 - 22081. [Abstract] [Full Text] [PDF]

				I. Ghosh, L. Sun, and M.-Q. Xu Zinc Inhibition of Protein trans-Splicing and Identification of Regions Essential for Splicing and Association of a Split Intein* J. Biol. Chem., June 22, 2001; 276(26): 24051 - 24058. [Abstract] [Full Text] [PDF]