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J. Biol. Chem., Vol. 265, Issue 13, 7248-7256, 05, 1990
JE Vance
A crude rat liver mitochondrial fraction that was capable of the rapid,
linked synthesis of phosphatidylserine (PtdSer), phosphatidylethanolamine
(PtdEtn), and phosphatidylcholine (PtdCho) labeled from [3-3H] serine has
been fractionated. PtdSer synthase, PtdEtn methyltransferase, and
CDP-choline:diacylglycerol cholinephosphotransferase activities were
present in the crude mitochondrial preparation but were absent from highly
purified mitochondria and could be attributed to the presence of a membrane
fraction, X. Thus, previous claims of the mitochondrial location of some of
these enzymes might be explained by the presence of fraction X in the
mitochondrial preparation. Fraction X had many similarities to microsomes
except that it sedimented with mitochondria (at 10,000 x g). However, the
specific activities of PtdSer synthase and glucose-6- phosphate phosphatase
in fraction X were almost twice that of microsomes, and the specific
activities of CTP:phosphocholine cytidylyltransferase and NADPH:cytochrome
c reductase in fraction X were much lower than in microsomes. The marker
enzymes for mitochondria, Golgi apparatus, plasma membrane, lysosomes, and
peroxisomes all had low activities in fraction X. Polyacrylamide gel
electrophoresis revealed distinct differences, as well as similarities,
among the proteins of fraction X, microsomes, and rough and smooth
endoplasmic reticulum. The combined mitochondria-fraction X membranes can
synthesize PtdSer, PtdEtn, and PtdCho from serine. Thus, fraction X in
combination with mitochondria might be responsible for the observed
compartmentalization of a serine-labeled pool of phospholipids previously
identified (Vance, J. E., and Vance, D. E. (1986) J. Biol. Chem. 261,
4486-4491) and might be involved in the transfer of lipids between the
endoplasmic reticulum and mitochondria.
Phospholipid synthesis in a membrane fraction associated with mitochondria
Department of Medicine, University of Alberta, Edmonton, Canada.
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