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J. Biol. Chem., Vol. 265, Issue 13, 7257-7259, May, 1990

Reappraisal of the effect of Ca2+ on the activity of liver microsomal glucose-6-phosphatase

G Mithieux, F Vega, M Beylot and JP Riou
Institut National de la Sante et de la Recherche Medicale, Unite 197, Faculte de Medecine Alexis Carrel, Lyon, France.

It has recently been reported that free Ca2+, a second hormonal messenger in the liver, can modulate the activity of liver glucose-6- phosphatase by inhibition (van de Werve, G. (1989) J. Biol. Chem. 264, 6033-6036) or activation (Yamagushi, M., Mori, S., and Suketa, Y. (1989) Chem. Pharm. Bull. (Tokyo) 37, 388-390). Such a controversial role for Ca2+ is reinvestigated by comparing the effect of the addition of free Ca2+ (10(-10) to 20.10(-3) M) under the form of CaCl2 or of Ca- EGTA buffers. We show that the glucose-6-phosphatase activity is: 1) increased in the presence of CaCl2 at concentrations higher than 10(-4) M and unaffected in the presence of CaCl2 at lower concentrations; 2) decreased in the presence of Ca-EGTA buffers yielding free Ca2+ concentrations higher than 10(-8) M; 3) the latter effect is not depending on free Ca2+ or free EGTA concentrations, but on Ca.EGTA complex concentration. In addition, these effects can be reproduced in the same concentration ranges by MgCl2 and Mg-EDTA buffers, respectively. It is concluded that a physiological role for free Ca2+ on the activity of liver glucose-6-phosphatase remains to be established.
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