HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schaap, D. Articles by Parker, P. J. Search for Related Content PubMed PubMed Citation Articles by Schaap, D. Articles by Parker, P. J. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 265, Issue 13, 7301-7307, May, 1990

Expression, purification, and characterization of protein kinase C- epsilon

D Schaap and PJ Parker
Ludwig Institute for Cancer Research, London, Great Britain.

Of the recently described members of the protein kinase C (PKC) family (-delta, -epsilon, -zeta), no detailed properties of the purified enzymes have been presented. Here we describe the expression of PKC- epsilon in insect cells using a baculovirus vector. The recombinant enzyme has been purified to homogeneity by sequential chromatography on DEAE-cellulose, serine-Sepharose, Mono Q, and Superose 12; the protein shows a molecular mass of 90 kDa on sodium dodecyl sulfate- polyacrylamide gel electrophoresis. PKC-epsilon is dependent upon phospholipid and diacylglycerol (or phorbol esters) for activity and displays a pattern of specificity for these effectors similar to other PKC isotypes. Similarly, inhibition of PKC-epsilon by staurosporine and H-7 parallels inhibition of other PKC isotypes. However, unlike PKC- alpha, -beta, and -gamma, PKC-epsilon shows no dependence upon Ca2+. Furthermore, the substrate specificity of PKC-epsilon is quite different from other characterized PKCs. The importance of functional diversity within the PKC family is discussed.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. J. Webb, J. D. Judah, L.-C. Lo, and G. M. H. Thomas Constitutive secretion of serum albumin requires reversible protein tyrosine phosphorylation events in trans-Golgi Am J Physiol Cell Physiol, September 1, 2005; 289(3): C748 - C756. [Abstract] [Full Text] [PDF]

				X. Huang and J. W. Walker Myofilament anchoring of protein kinase C-epsilon in cardiac myocytes J. Cell Sci., April 15, 2004; 117(10): 1971 - 1978. [Abstract] [Full Text] [PDF]

				S. E. Alpert, R. W. Walenga, A. Mandal, N. Bourbon, and M. Kester 15-HETE-substituted diglycerides selectively regulate PKC isotypes in human tracheal epithelial cells Am J Physiol Lung Cell Mol Physiol, September 1, 1999; 277(3): L457 - L464. [Abstract] [Full Text] [PDF]

				A. Asada, Y. Zhao, S. Kondo, and M. Iwata Induction of Thymocyte Apoptosis by Ca2+-independent Protein Kinase C (nPKC) Activation and Its Regulation by Calcineurin Activation J. Biol. Chem., October 23, 1998; 273(43): 28392 - 28398. [Abstract] [Full Text] [PDF]

				P. Sil, V. Kandaswamy, and S. Sen Increased Protein Kinase C Activity in Myotrophin-Induced Myocyte Growth Circ. Res., June 15, 1998; 82(11): 1173 - 1188. [Abstract] [Full Text] [PDF]

				S. F. Pietromonaco, P. C. Simons, A. Altman, and L. Elias Protein Kinase C-theta Phosphorylation of Moesin in the Actin-binding Sequence J. Biol. Chem., March 27, 1998; 273(13): 7594 - 7603. [Abstract] [Full Text] [PDF]

				P. Saharinen, N. Ekman, K. Sarvas, P. Parker, K. Alitalo, and O. Silvennoinen The Bmx Tyrosine Kinase Induces Activation of the Stat Signaling Pathway, Which Is Specifically Inhibited by Protein Kinase Cdelta Blood, December 1, 1997; 90(11): 4341 - 4353. [Abstract] [Full Text] [PDF]

				P. Ping, J. Zhang, Y. Qiu, X.-L. Tang, S. Manchikalapudi, X. Cao, and R. Bolli Ischemic Preconditioning Induces Selective Translocation of Protein Kinase C Isoforms {epsilon} and {eta} in the Heart of Conscious Rabbits Without Subcellular Redistribution of Total Protein Kinase C Activity Circ. Res., September 19, 1997; 81(3): 404 - 414. [Abstract] [Full Text]

				K. Kielbassa, H.-J. Müller, H. E. Meyer, F. Marks, and M. Gschwendt Protein Kinase C[IMAGE]-specific Phosphorylation of the Elongation Factor eEF-1alpha and an eEF-1alpha Peptide at Threonine 431 J. Biol. Chem., March 17, 1995; 270(11): 6156 - 6162. [Abstract] [Full Text] [PDF]

				S. Kiley and P. Parker Differential localization of protein kinase C isozymes in U937 cells: evidence for distinct isozyme functions during monocyte differentiation J. Cell Sci., January 3, 1995; 108(3): 1003 - 1016. [Abstract] [PDF]

				Y Nishizuka Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C Science, October 23, 1992; 258(5082): 607 - 614. [Abstract] [PDF]