HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yamada, M. Articles by Okazaki, T. Search for Related Content PubMed PubMed Citation Articles by Yamada, M. Articles by Okazaki, T. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 265, Issue 13, 7687-7692, May, 1990

Structural organization of the rat cytochrome c oxidase subunit IV gene

M Yamada, N Amuro, Y Goto and T Okazaki
Department of Biochemistry, Nippon Medical School, Tokyo, Japan.

We have isolated two overlapping clones covering the entire length of the gene of nuclear-encoded sub-unit IV of cytochrome c oxidase (COXIV) from a rat genomic library in Charon 4A and determined the structural organization of the gene. The gene spans approximately 7.0 kilobases and contains five exons interrupted by four introns. Of these exons, exon 2 codes for a whole length of the presequence of the rat COXIV precursor protein, while exons 3 to 5 encode three distinct structural domains of the mature protein. The 5'-flanking region of the gene lacks conventional TATA and CAAT boxes, but has a high G + C content and contains two putative binding sites for transcription factor SP1 and a sequence resembling the AP-4 responsive element. These results indicate that the promoter region of the rat COXIV gene possesses characteristic features common in housekeeping genes. The chloramphenicol acetyltransferase assay performed by constructing an improved phagemid, pBlueCAT3, revealed that a 773-base pair DNA fragment immediately preceding the cap site has a strong promoter activity. An octanucleotide sequence, -TTCTTGGT-, which is very close to the yeast HAP2/HAP3 responsive element, is located in the 5'-upstream region of the present gene.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				N. Lenka, A. Basu, J. Mullick, and N. G. Avadhani The Role of an E Box Binding Basic Helix Loop Helix Protein in the Cardiac Muscle-specific Expression of the Rat Cytochrome Oxidase Subunit VIII Gene J. Biol. Chem., November 22, 1996; 271(47): 30281 - 30289. [Abstract] [Full Text] [PDF]