| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 265, Issue 14, 7733-7736, 05, 1990
AD Richards, LH Phylip, WG Farmerie, PE Scarborough, A Alvarez, BM Dunn, PH Hirel, J Konvalinka, P Strop and L Pavlickova
By replacement of the P1' residue in a capsid/nucleocapsid cleavage site
mimic with 4-NO2-phenylalanine (Nph), an excellent chromogenic substrate,
Lys-Ala-Arg-Val-Leu*Nph-Glu-Ala-Met, for HIV-1 proteinase (kappa cat = 20
s-1, Km = 22 microM) has been prepared. Substitution of the Leu residue in
P1 with norleucine, Met, Phe, or Tyr had minimal effects on the kinetic
parameters (kappa cat and kappa cat/Km) determined at different pH values,
whereas peptides containing Ile or Val in P1 were hydrolyzed extremely
slowly. The spectrophotometric assay has been used to characterize the
proteinase further with respect to pH dependence, ionic strength
dependence, and the effect of competitive inhibitors of various types.
Sensitive, soluble chromogenic substrates for HIV-1 proteinase
Department of Biochemistry, University of Wales College of Cardiff, United Kingdom.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. G. Saskova, M. Kozisek, M. Lepsik, J. Brynda, P. Rezacova, J. Vaclavikova, R. M. Kagan, L. Machala, and J. Konvalinka Enzymatic and structural analysis of the I47A mutation contributing to the reduced susceptibility to HIV protease inhibitor lopinavir Protein Sci., September 1, 2008; 17(9): 1555 - 1564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ishima, D. A. Torchia, and J. M. Louis Mutational and Structural Studies Aimed at Characterizing the Monomer of HIV-1 Protease and Its Precursor J. Biol. Chem., June 8, 2007; 282(23): 17190 - 17199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kawe, P. Forrer, P. Amstutz, and A. Pluckthun Isolation of Intracellular Proteinase Inhibitors Derived from Designed Ankyrin Repeat Proteins by Genetic Screening J. Biol. Chem., December 29, 2006; 281(52): 40252 - 40263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Pettit, J. C. Clemente, J. A. Jeung, B. M. Dunn, and A. H. Kaplan Ordered Processing of the Human Immunodeficiency Virus Type 1 GagPol Precursor Is Influenced by the Context of the Embedded Viral Protease J. Virol., August 15, 2005; 79(16): 10601 - 10607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chatterjee, P. Mridula, R. K. Mishra, R. Mittal, and R. V. Hosur Folding Regulates Autoprocessing of HIV-1 Protease Precursor J. Biol. Chem., March 25, 2005; 280(12): 11369 - 11378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Pettit, L. E. Everitt, S. Choudhury, B. M. Dunn, and A. H. Kaplan Initial Cleavage of the Human Immunodeficiency Virus Type 1 GagPol Precursor by Its Activated Protease Occurs by an Intramolecular Mechanism J. Virol., August 15, 2004; 78(16): 8477 - 8485. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ishima, D. A. Torchia, S. M. Lynch, A. M. Gronenborn, and J. M. Louis Solution Structure of the Mature HIV-1 Protease Monomer: INSIGHT INTO THE TERTIARY FOLD AND STABILITY OF A PRECURSOR J. Biol. Chem., October 31, 2003; 278(44): 43311 - 43319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Kohler A yeast-based growth assay for the analysis of site-specific proteases Nucleic Acids Res., February 15, 2003; 31(4): e16 - e16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Louis, R. Ishima, I. Nesheiwat, L. K. Pannell, S. M. Lynch, D. A. Torchia, and A. M. Gronenborn Revisiting Monomeric HIV-1 Protease. CHARACTERIZATION AND REDESIGN FOR IMPROVED PROPERTIES J. Biol. Chem., February 14, 2003; 278(8): 6085 - 6092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Pettit, G. J. Henderson, C. A. Schiffer, and R. Swanstrom Replacement of the P1 Amino Acid of Human Immunodeficiency Virus Type 1 Gag Processing Sites Can Inhibit or Enhance the Rate of Cleavage by the Viral Protease J. Virol., September 11, 2002; 76(20): 10226 - 10233. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Fodor and V. M. Vogt Characterization of the Protease of a Fish Retrovirus, Walleye Dermal Sarcoma Virus J. Virol., March 27, 2002; 76(9): 4341 - 4349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Dauber, R. Ziermann, N. Parkin, D. J. Maly, S. Mahrus, J. L. Harris, J. A. Ellman, C. Petropoulos, and C. S. Craik Altered Substrate Specificity of Drug-Resistant Human Immunodeficiency Virus Type 1 Protease J. Virol., February 1, 2002; 76(3): 1359 - 1368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-C. Lin, Z. Beck, T. Lee, V.-D. Le, G. M. Morris, A. J. Olson, C.-H. Wong, and J. H. Elder Alteration of Substrate and Inhibitor Specificity of Feline Immunodeficiency Virus Protease J. Virol., May 15, 2000; 74(10): 4710 - 4720. [Abstract] [Full Text]
|
|
|
|
|
|
C. Paulus, S. Hellebrand, U. Tessmer, H. Wolf, H.-G. Krausslich, and R. Wagner Competitive Inhibition of Human Immunodeficiency Virus Type-1 Protease by the Gag-Pol Transframe Protein J. Biol. Chem., July 30, 1999; 274(31): 21539 - 21543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Davis, K. Yusa, L. A. Gillim, F. M. Newcomb, H. Mitsuya, and R. Yarchoan Conserved Cysteines of the Human Immunodeficiency Virus Type 1 Protease Are Involved in Regulation of Polyprotein Processing and Viral Maturation of Immature Virions J. Virol., February 1, 1999; 73(2): 1156 - 1164. [Abstract] [Full Text]
|
|
|
|
|
|
K. Wiegers, G. Rutter, H. Kottler, U. Tessmer, H. Hohenberg, and H.-G. Krausslich Sequential Steps in Human Immunodeficiency Virus Particle Maturation Revealed by Alterations of Individual Gag Polyprotein Cleavage Sites J. Virol., April 1, 1998; 72(4): 2846 - 2854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pazhanisamy, C. M. Stuver, A. B. Cullinan, N. Margolin, B. G. Rao, and D. J. Livingston Kinetic Characterization of Human Immunodeficiency Virus Type-1 Protease-resistant Variants J. Biol. Chem., July 26, 1996; 271(30): 17979 - 17985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Józ. Tözsér, Pét. Bagossi, I. T. Weber, T. D. Copeland, and S. Oroszlan Comparative Studies on the Substrate Specificity of Avian Myeloblastosis Virus Proteinase and Lentiviral Proteinases J. Biol. Chem., March 22, 1996; 271(12): 6781 - 6788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.án Szeltner and Lás. Polgár Conformational Stability and Catalytic Activity of HIV-1 Protease Are Both Enhanced at High Salt Concentration J. Biol. Chem., March 8, 1996; 271(10): 5458 - 5463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Hrusková-Heidingsfeldová, M. Andreansky, M. Fábry, I. Bláha, P. Strop, and E. Hunter Cloning, Bacterial Expression, and Characterization of the Mason-Pfizer Monkey Virus Proteinase J. Biol. Chem., June 23, 1995; 270(25): 15053 - 15058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Dash and M. Rao Interactions of a Novel Inhibitor from an Extremophilic Bacillus sp. with HIV-1 Protease. IMPLICATIONS FOR THE MECHANISM OF INACTIVATION J. Biol. Chem., January 19, 2001; 276(4): 2487 - 2493. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
