HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Meichle, A. Articles by Kronke, M. Search for Related Content PubMed PubMed Citation Articles by Meichle, A. Articles by Kronke, M. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 265, Issue 14, 8339-8343, May, 1990

Protein kinase C-independent activation of nuclear factor kappa B by tumor necrosis factor

A Meichle, S Schutze, G Hensel, D Brunsing and M Kronke
Klinische Arbeitsgruppe der Max-Planck-Gesellschaft, Gottingen, Federal Republic of Germany.

Tumor necrosis factor (TNF) is one of the few physiological inducers of the pleiotropic transcription factor NF kappa B. NF kappa B may play a central role in mediating TNF's gene regulatory action; however, the molecular mechanisms of TNF-induced NF kappa B activation are poorly understood. In this study, we demonstrate with two human leukemic cell lines, K562 and Jurkat, that TNF induces rapid and transient activation and translocation of protein kinase C (PK-C) from the cytosol to the membranes, which is followed by the emergence of kappa B-binding activity. In order to investigate whether TNF-mediated PK-C activation can be linked to induction of NF kappa B, we tried to block TNF action by use of various protein kinase C inhibitors as well as down- regulation of PK-C. Preincubation of Jurkat cells with protein kinase inhibitor H7 or staurosporine blocked PK-C activation by either TNF or phorbol 12-myristate 12-acetate (PMA). This pretreatment regimen completely inhibited NF kappa B activation by PMA. In contrast, TNF's ability to induce NF kappa B remained unaffected. In addition, NF kappa B was TNF-inducible in Jurkat cells depleted for PK-C by long-term exposure to high dose phorbol ester. The data indicate that PK-C is not required for NF kappa B activation by TNF and imply a novel, PK-C- independent mechanism of physiological NF kappa B activation.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. L. Kim, B. Zhang, I. P. Mills, M. E. Milla, K. R. Brunden, and V. M.-Y. Lee Effects of TNF{alpha}-Converting Enzyme Inhibition on Amyloid {beta} Production and APP Processing In Vitro and In Vivo J. Neurosci., November 12, 2008; 28(46): 12052 - 12061. [Abstract] [Full Text] [PDF]

				S. C. Morley, K. S. Weber, H. Kao, and P. M. Allen Protein Kinase C-{theta} Is Required for Efficient Positive Selection J. Immunol., October 1, 2008; 181(7): 4696 - 4708. [Abstract] [Full Text] [PDF]

				N. Abboushi, A. El-Hed, W. El-Assaad, L. Kozhaya, M. E. El-Sabban, A. Bazarbachi, R. Badreddine, A. Bielawska, J. Usta, and G. S. Dbaibo Ceramide Inhibits IL-2 Production by Preventing Protein Kinase C-Dependent NF-{kappa}B Activation: Possible Role in Protein Kinase C{theta} Regulation J. Immunol., September 1, 2004; 173(5): 3193 - 3200. [Abstract] [Full Text] [PDF]

				V. Matthews, B. Schuster, S. Schutze, I. Bussmeyer, A. Ludwig, C. Hundhausen, T. Sadowski, P. Saftig, D. Hartmann, K.-J. Kallen, et al. Cellular Cholesterol Depletion Triggers Shedding of the Human Interleukin-6 Receptor by ADAM10 and ADAM17 (TACE) J. Biol. Chem., October 3, 2003; 278(40): 38829 - 38839. [Abstract] [Full Text] [PDF]

				B. Madan, S. Batra, and B. Ghosh 2'-Hydroxychalcone Inhibits Nuclear Factor-kappa B and Blocks Tumor Necrosis Factor-alpha - and Lipopolysaccharide-Induced Adhesion of Neutrophils to Human Umbilical Vein Endothelial Cells Mol. Pharmacol., September 1, 2000; 58(3): 526 - 534. [Abstract] [Full Text]

				M. Ruiz-Ortega, O. Lorenzo, M. Ruperez, S. Konig, B. Wittig, and J. Egido Angiotensin II Activates Nuclear Transcription Factor {kappa}B Through AT1 and AT2 in Vascular Smooth Muscle Cells : Molecular Mechanisms Circ. Res., June 23, 2000; 86(12): 1266 - 1272. [Abstract] [Full Text] [PDF]

				V. Machelon, F. Nomé, and D. Emilie Regulated on Activation Normal T Expressed and Secreted Chemokine Is Induced by Tumor Necrosis Factor-{alpha} in Granulosa Cells from Human Preovulatory Follicle J. Clin. Endocrinol. Metab., January 1, 2000; 85(1): 417 - 424. [Abstract] [Full Text]

				S. A. Trushin, K. N. Pennington, A. Algeciras-Schimnich, and C. V. Paya Protein Kinase C and Calcineurin Synergize to Activate Ikappa B Kinase and NF-kappa B in T Lymphocytes J. Biol. Chem., August 13, 1999; 274(33): 22923 - 22931. [Abstract] [Full Text] [PDF]

				R. Zidovetzki, J.-L. Wang, J. A. Kim, P. Chen, M. Fisher, and F. M. Hofman Endothelin-1 Enhances Plasminogen Activator Inhibitor-1 Production by Human Brain Endothelial Cells via Protein Kinase C-Dependent Pathway Arterioscler. Thromb. Vasc. Biol., July 1, 1999; 19(7): 1768 - 1775. [Abstract] [Full Text] [PDF]

				Y. Han, T. Meng, N. R. Murray, A. P. Fields, and A. R. Brasier Interleukin-1-induced Nuclear Factor-kappa B-Ikappa Balpha Autoregulatory Feedback Loop in Hepatocytes. A ROLE FOR PROTEIN KINASE Calpha IN POST-TRANSCRIPTIONAL REGULATION OF Ikappa Balpha RESYNTHESIS J. Biol. Chem., January 8, 1999; 274(2): 939 - 947. [Abstract] [Full Text] [PDF]

				S. K. Manna and B. B. Aggarwal {alpha}-Melanocyte-Stimulating Hormone Inhibits the Nuclear Transcription Factor NF-{kappa}B Activation Induced by Various Inflammatory Agents J. Immunol., September 15, 1998; 161(6): 2873 - 2880. [Abstract] [Full Text] [PDF]

				H. Nakamura, K. J. Haley, T. Nakamura, A. D. Luster, and C. M. Lilly Differential regulation of eotaxin expression by TNF-alpha and PMA in human monocytic U-937 cells Am J Physiol Lung Cell Mol Physiol, September 1, 1998; 275(3): L601 - L610. [Abstract] [Full Text] [PDF]

				M. Hiramoto, N. Shimizu, K. Sugimoto, J. Tang, Y. Kawakami, M. Ito, S. Aizawa, H. Tanaka, I. Makino, and H. Handa Nuclear Targeted Suppression of NF-{kappa}B Activity by the Novel Quinone Derivative E3330 J. Immunol., January 15, 1998; 160(2): 810 - 819. [Abstract] [Full Text] [PDF]

				W. S. Lai, M. J. Thompson, and P. J. Blackshear Characteristics of the Intron Involvement in the Mitogen-induced Expression of Zfp-36 J. Biol. Chem., January 2, 1998; 273(1): 506 - 517. [Abstract] [Full Text] [PDF]

				U. R. Pendurthi, J. T. Williams, and L. V. M. Rao Inhibition of Tissue Factor Gene Activation in Cultured Endothelial Cells by Curcumin : Suppression of Activation of Transcription Factors Egr-1, AP-1, and NF-{kappa}B Arterioscler. Thromb. Vasc. Biol., December 1, 1997; 17(12): 3406 - 3413. [Abstract] [Full Text]

				S. A. G. Reddy, J. H. Huang, and W. S.-L. Liao Phosphatidylinositol 3-Kinase in Interleukin 1 Signaling. PHYSICAL INTERACTION WITH THE INTERLEUKIN 1 RECEPTOR AND REQUIREMENT IN NFkappa B AND AP-1 ACTIVATION J. Biol. Chem., November 14, 1997; 272(46): 29167 - 29173. [Abstract] [Full Text] [PDF]

				J. J. Breton and M. C. Chabot-Fletcher The Natural Product Hymenialdisine Inhibits Interleukin-8 Production in U937 Cells by Inhibition of Nuclear Factor-kappa B J. Pharmacol. Exp. Ther., July 1, 1997; 282(1): 459 - 466. [Abstract] [Full Text]

				A. R. Brasier, J. Li, and K. A. Wimbish Tumor Necrosis Factor Activates Angiotensinogen Gene Expression by the Rel A Transactivator Hypertension, April 1, 1996; 27(4): 1009 - 1017. [Abstract] [Full Text]

				S. Maruyama, T. Kurosaki, K. Sada, Y. Yamanashi, T. Yamamoto, and H. Yamamura Physical and Functional Association of Cortactin with Syk in Human Leukemic Cell Line K562 J. Biol. Chem., March 22, 1996; 271(12): 6631 - 6635. [Abstract] [Full Text] [PDF]

				A. R. Brasier and J. Li Mechanisms for Inducible Control of Angiotensinogen Gene Transcription Hypertension, March 1, 1996; 27(3): 465 - 475. [Abstract] [Full Text]

				A. Lægreid, L. Thommesen, T. G. Jahr, A. Sundan, and T. Espevik Tumor Necrosis Factor Induces Lipopolysaccharide Tolerance in a Human Adenocarcinoma Cell Line Mainly through the TNF p55 Receptor J. Biol. Chem., October 27, 1995; 270(43): 25418 - 25425. [Abstract] [Full Text] [PDF]

				S. Singh and B. B. Aggarwal Activation of Transcription Factor NF-kappaB Is Suppressed by Curcumin (Diferuloylmethane) J. Biol. Chem., October 20, 1995; 270(42): 24995 - 25000. [Abstract] [Full Text] [PDF]

				M. Muroi, Y. Muroi, N. Ito, N.R. Rice, and T. Suzuki Effects of protease inhibitors on LPS-mediated activation of a mouse macrophage cell line (J774) Innate Immunity, October 1, 1995; 2(5): 337 - 347. [Abstract] [PDF]

				P. Wang, P. Wu, M. I. Siegel, R. W. Egan, and M. M. Billah Interleukin (IL)-10 Inhibits Nuclear Factor [IMAGE]B (NF[IMAGE]B) Activation in Human Monocytes J. Biol. Chem., April 21, 1995; 270(16): 9558 - 9563. [Abstract] [Full Text] [PDF]