HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Blackhart, B. D. Articles by McCarthy, B. J. Search for Related Content PubMed PubMed Citation Articles by Blackhart, B. D. Articles by McCarthy, B. J. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 265, Issue 15, 8358-8360, May, 1990

An expression system for human apolipoprotein B100 in a rat hepatoma cell line

BD Blackhart, ZM Yao and BJ McCarthy
Gladstone Foundation Laboratories for Cardiovascular Disease, University of California, San Francisco 94140-0608.

We have designed an in vitro expression system for human apolipoprotein (apo) B. A full-length human apoB minigene was constructed from cDNA and genomic apoB clones and inserted into a vector where its expression was directed by the cytomegalovirus promoter. The apoB minigene was expressed in a rat hepatoma cell line, McA-RH7777. Human apoB100, which is the ligand for the low density lipoprotein receptor, was secreted in low density lipoprotein or very low density lipoprotein particles, depending on the composition of the medium. A protein with the mobility of apoB48, a structurally related protein involved in cholesterol metabolism, was also produced from the human apoB minigene. This in vitro expression system for human apoB will enable investigators to identify which domains of this protein are involved in processes such as lipoprotein assembly and secretion. This system should also allow investigators to identify definitively the domain in apoB that enables the protein to bind to the low density lipoprotein receptor.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. R. Burnett, S. Zhong, Z. G. Jiang, A. J. Hooper, E. A. Fisher, R. S. McLeod, Y. Zhao, P. H. R. Barrett, R. A. Hegele, F. M. van Bockxmeer, et al. Missense Mutations in APOB within the beta{alpha}1 Domain of Human APOB-100 Result in Impaired Secretion of ApoB and ApoB-containing Lipoproteins in Familial Hypobetalipoproteinemia J. Biol. Chem., August 17, 2007; 282(33): 24270 - 24283. [Abstract] [Full Text] [PDF]

				R. J. Brown, J. R. Schultz, K. W. S. Ko, J. S. Hill, T. A. Ramsamy, A. L. White, D. L. Sparks, and Z. Yao The amino acid sequences of the carboxyl termini of human and mouse hepatic lipase influence cell surface association J. Lipid Res., July 1, 2003; 44(7): 1306 - 1314. [Abstract] [Full Text] [PDF]

				J. Vukmirica, K. Tran, X. Liang, J. Shan, J. Yuan, B. A. Miskie, R. A. Hegele, M. D. Resh, and Z. Yao Assembly and Secretion of Very Low Density Lipoproteins Containing Apolipoprotein B48 in Transfected McA-RH7777 Cells. LACK OF EVIDENCE THAT PALMITOYLATION OF APOLIPOPROTEIN B48 IS REQUIRED FOR LIPOPROTEIN SECRETION J. Biol. Chem., April 11, 2003; 278(16): 14153 - 14161. [Abstract] [Full Text] [PDF]

				J. R. Burnett, J. Shan, B. A. Miskie, A. J. Whitfield, J. Yuan, K. Tran, C. J. McKnight, R. A. Hegele, and Z. Yao A Novel Nontruncating APOB Gene Mutation, R463W, Causes Familial Hypobetalipoproteinemia J. Biol. Chem., April 4, 2003; 278(15): 13442 - 13452. [Abstract] [Full Text] [PDF]

				A. Gauthier, G. Vassiliou, F. Benoist, and R. McPherson Adipocyte Low Density Lipoprotein Receptor-related Protein Gene Expression and Function Is Regulated by Peroxisome Proliferator-activated Receptor gamma J. Biol. Chem., March 28, 2003; 278(14): 11945 - 11953. [Abstract] [Full Text] [PDF]

				X. Wang, V. Chauhan, A. T. Nguyen, J. Schultz, J. Davignon, S. G. Young, J. Boren, T. L. Innerarity, H. Rutai, and R. W. Milne Immunochemical evidence that human apoB differs when expressed in rodent versus human cells J. Lipid Res., March 1, 2003; 44(3): 547 - 553. [Abstract] [Full Text] [PDF]

				J. Vukmirica, T. Nishimaki-Mogami, K. Tran, J. Shan, R. S. McLeod, J. Yuan, and Z. Yao The N-linked oligosaccharides at the amino terminus of human apoB are important for the assembly and secretion of VLDL J. Lipid Res., September 1, 2002; 43(9): 1496 - 1507. [Abstract] [Full Text] [PDF]

				A. Kulinski, S. Rustaeus, and J. E. Vance Microsomal Triacylglycerol Transfer Protein Is Required for Lumenal Accretion of Triacylglycerol Not Associated with ApoB, as Well as for ApoB Lipidation J. Biol. Chem., August 23, 2002; 277(35): 31516 - 31525. [Abstract] [Full Text] [PDF]

				Y. Zhao, J. B. McCabe, J. Vance, and L. G. Berthiaume Palmitoylation of Apolipoprotein B Is Required for Proper Intracellular Sorting and Transport of Cholesteroyl Esters and Triglycerides Mol. Biol. Cell, February 1, 2000; 11(2): 721 - 734. [Abstract] [Full Text]

				B. Gauthier, M. Robb, F. Gaudet, G. S. Ginsburg, and R. McPherson Characterization of a cholesterol response element (CRE) in the promoter of the cholesteryl ester transfer protein gene: functional role of the transcription factors SREBP-1a, -2, and YY1. J. Lipid Res., July 1, 1999; 40(7): 1284 - 1293. [Abstract] [Full Text]

				J. Wu, J. Kim, Q. Li, P.-Y. Kwok, T. G. Cole, B. Cefalu, M. Averna, and G. Schonfeld Known mutations of apoB account for only a small minority of hypobetalipoproteinemia J. Lipid Res., May 1, 1999; 40(5): 955 - 959. [Abstract] [Full Text]

				E. Kim and S. G. Young Genetically modified mice for the study of apolipoprotein B J. Lipid Res., April 1, 1998; 39(4): 703 - 723. [Abstract] [Full Text] [PDF]

				S.-L. Wang, E. Z. Du, T. D. Martin, and R. A. Davis Coordinate Regulation of Lipogenesis, the Assembly and Secretion of Apolipoprotein B-containing Lipoproteins by Sterol Response Element Binding Protein 1 J. Biol. Chem., August 1, 1997; 272(31): 19351 - 19358. [Abstract] [Full Text] [PDF]

				C. L. Baum, E. J. Reschly, A. K. Gayen, M. E. Groh, and K. Schadick Sterol Carrier Protein-2 Overexpression Enhances Sterol Cycling and Inhibits Cholesterol Ester Synthesis and High Density Lipoprotein Cholesterol Secretion J. Biol. Chem., March 7, 1997; 272(10): 6490 - 6498. [Abstract] [Full Text] [PDF]

				R. S. McLeod, Y. Wang, S. Wang, A. Rusinol, P. Links, and Z. Yao Apolipoprotein B Sequence Requirements for Hepatic Very Low Density Lipoprotein Assembly. EVIDENCE THAT HYDROPHOBIC SEQUENCES WITHIN APOLIPOPROTEIN B48 MEDIATE LIPID RECRUITMENT J. Biol. Chem., August 2, 1996; 271(31): 18445 - 18455. [Abstract] [Full Text] [PDF]

				S. Wang, R. S. McLeod, D. A. Gordon, and Z. Yao The Microsomal Triglyceride Transfer Protein Facilitates Assembly and Secretion of Apolipoprotein B-containing Lipoproteins and Decreases Cotranslational Degradation of Apolipoprotein B in Transfected COS-7 Cells J. Biol. Chem., June 14, 1996; 271(24): 14124 - 14133. [Abstract] [Full Text] [PDF]

				D. G. Gretch, S. L. Sturley, L. Wang, B. A. Lipton, A. Dunning, K. A. A. Grunwald, J. R. Wetterau, Z. Yao, P. Talmud, and A. D. Attie The Amino Terminus of Apolipoprotein B Is Necessary but Not Sufficient for Microsomal Triglyceride Transfer Protein Responsiveness J. Biol. Chem., April 12, 1996; 271(15): 8682 - 8691. [Abstract] [Full Text] [PDF]

				M. M. Hussain, Y. Zhao, R. K. Kancha, B. D. Blackhart, and Z. Yao Characterization of Recombinant Human ApoB-48–Containing Lipoproteins in Rat Hepatoma McA-RH7777 Cells Transfected With ApoB-48 cDNA : Overexpression of ApoB-48 Decreases Synthesis of Endogenous ApoB-100 Arterioscler. Thromb. Vasc. Biol., April 1, 1995; 15(4): 485 - 494. [Abstract] [Full Text]