|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 265, Issue 15, 8550-8557, 05, 1990
K Furuno, MN Goodman and AL Goldberg
In order to clarify the cellular mechanisms of denervation atrophy of
skeletal muscle, we have studied protein turnover in denervated and control
rat soleus muscles in vitro under different conditions. By 24 h after
cutting the sciatic nerve, overall protein breakdown was greater in the
denervated soleus than in the contralateral control muscle, and by 3 days,
net proteolysis had increased about 3-fold. Since protein synthesis
increased slightly following denervation, the rise in proteolysis must be
responsible for the muscle atrophy and the differential loss of contractile
proteins. Like overall proteolysis, the breakdown of actin (as shown by
3-methyl-histidine production by the muscles) increased each day after
denervation and by 3 days was 2.5 times faster than in controls. Treatments
that block the lysosomal and Ca2(+)-dependent proteolytic systems did not
reduce the increase in overall protein degradation and actin breakdown in
the denervated muscles (maintained in complete medium at resting length).
However, the content of the lysosomal protease, cathepsin B, increased
about 2-fold by 3 days after denervation. Furthermore, conditions that
activate intralysosomal proteolysis (incubation without insulin or amino
acids) stimulated proteolysis 2-3-fold more in the denervated muscles than
in controls. Also, incubation conditions that activate the Ca2(+)-
dependent pathway (incubation with Ca2+ ionophores or allowing muscles to
shorten) were 2-3 times more effective in enhancing overall proteolysis in
the denervated muscle. None of these treatments affected 3-methylhistidine
production. Thus, multiple proteolytic systems increase in parallel in the
denervated muscle, but a nonlysosomal process (independent of Ca2+) appears
mainly responsible for the rapid loss of cell proteins, especially of
myofibrillar components.
Role of different proteolytic systems in the degradation of muscle proteins during denervation atrophy
Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts 02115.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Sandri Signaling in Muscle Atrophy and Hypertrophy Physiology, June 1, 2008; 23(3): 160 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. McClung, M. A. Whidden, A. N. Kavazis, D. J. Falk, K. C. DeRuisseau, and S. K. Powers Redox regulation of diaphragm proteolysis during mechanical ventilation Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2008; 294(5): R1608 - R1617. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Kavazis, K. C. DeRuisseau, J. M. McClung, M. A. Whidden, D. J. Falk, A. J. Smuder, T. Sugiura, and S. K. Powers Muscle: Diaphragmatic proteasome function is maintained in the ageing Fisher 344 rat Exp Physiol, September 1, 2007; 92(5): 895 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Koncarevic, R. W. Jackman, and S. C. Kandarian The ubiquitin-protein ligase Nedd4 targets Notch1 in skeletal muscle and distinguishes the subset of atrophies caused by reduced muscle tension FASEB J, February 1, 2007; 21(2): 427 - 437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Sacheck, J.-P. K. Hyatt, A. Raffaello, R. T. Jagoe, R. R. Roy, V. R. Edgerton, S. H. Lecker, and A. L. Goldberg Rapid disuse and denervation atrophy involve transcriptional changes similar to those of muscle wasting during systemic diseases FASEB J, January 1, 2007; 21(1): 140 - 155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Judge, A. Koncarevic, R. B. Hunter, H.-C. Liou, R. W. Jackman, and S. C. Kandarian Role for I{kappa}B{alpha}, but not c-Rel, in skeletal muscle atrophy Am J Physiol Cell Physiol, January 1, 2007; 292(1): C372 - C382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bartoli, N. Bourg, D. Stockholm, F. Raynaud, A. Delevacque, Y. Han, P. Borel, K. Seddik, N. Armande, and I. Richard A Mouse Model for Monitoring Calpain Activity under Physiological and Pathological Conditions J. Biol. Chem., December 22, 2006; 281(51): 39672 - 39680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sandri, J. Lin, C. Handschin, W. Yang, Z. P. Arany, S. H. Lecker, A. L. Goldberg, and B. M. Spiegelman PGC-1{alpha} protects skeletal muscle from atrophy by suppressing FoxO3 action and atrophy-specific gene transcription PNAS, October 31, 2006; 103(44): 16260 - 16265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Beehler, P. G. Sleph, L. Benmassaoud, and G. J. Grover Reduction of skeletal muscle atrophy by a proteasome inhibitor in a rat model of denervation. Experimental Biology and Medicine, March 1, 2006; 231(3): 335 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. DeRuisseau, A. N. Kavazis, M. A. Deering, D. J. Falk, D. Van Gammeren, T. Yimlamai, G. A. Ordway, and S. K. Powers Mechanical ventilation induces alterations of the ubiquitin-proteasome pathway in the diaphragm J Appl Physiol, April 1, 2005; 98(4): 1314 - 1321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Sacheck, A. Ohtsuka, S. C. McLary, and A. L. Goldberg IGF-I stimulates muscle growth by suppressing protein breakdown and expression of atrophy-related ubiquitin ligases, atrogin-1 and MuRF1 Am J Physiol Endocrinol Metab, October 1, 2004; 287(4): E591 - E601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kikuchi, T. Nakamura, S. Takayama, Y. Horiuchi, and Y. Toyama MR Imaging in the Diagnosis of Denervated and Reinnervated Skeletal Muscles: Experimental Study in Rats Radiology, December 1, 2003; 229(3): 861 - 867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Zdanowicz and S. Teichberg Effects of Insulin-Like Growth Factor-1/Binding Protein-3 Complex on Muscle Atrophy in Rats Experimental Biology and Medicine, September 1, 2003; 228(8): 891 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. E. Helman, E. Huff-Lonergan, G. M. Davenport, and S. M. Lonergan Effect of dietary protein on calpastatin in canine skeletal muscle J Anim Sci, September 1, 2003; 81(9): 2199 - 2205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Shanely, M. A. Zergeroglu, S. L. Lennon, T. Sugiura, T. Yimlamai, D. Enns, A. Belcastro, and S. K. Powers Mechanical Ventilation-induced Diaphragmatic Atrophy Is Associated with Oxidative Injury and Increased Proteolytic Activity Am. J. Respir. Crit. Care Med., November 15, 2002; 166(10): 1369 - 1374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. J. Adegoke, N. Bedard, H. P. Roest, and S. S. Wing Ubiquitin-conjugating enzyme E214k/HR6B is dispensable for increased protein catabolism in muscle of fasted mice Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E482 - E489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. Szewczyk, B. K. Peterson, and L. A. Jacobson Activation of Ras and the Mitogen-Activated Protein Kinase Pathway Promotes Protein Degradation in Muscle Cells of Caenorhabditis elegans Mol. Cell. Biol., June 15, 2002; 22(12): 4181 - 4188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Stockholm, M. Herasse, S. Marchand, C. Praud, C. Roudaut, I. Richard, A. Sebille, and J. S. Beckmann Calpain 3 mRNA expression in mice after denervation and during muscle regeneration Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1561 - C1569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Sneddon, M. I. Delday, and C. A. Maltin Amelioration of denervation-induced atrophy by clenbuterol is associated with increased PKC-alpha activity Am J Physiol Endocrinol Metab, July 1, 2000; 279(1): E188 - E195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Szewczyk, J. Hartman, S. Barmada, and L. Jacobson Genetic defects in acetylcholine signalling promote protein degradation in muscle cells of Caenorhabditis elegans J. Cell Sci., January 6, 2000; 113(11): 2003 - 2010. [Abstract] [PDF]
|
|
|
|
|
|
J. Huang and N. E. Forsberg Role of calpain in skeletal-muscle protein degradation PNAS, October 13, 1998; 95(21): 12100 - 12105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Franklin and E. M. Johnson Jr. Control of Neuronal Size Homeostasis by Trophic Factor-mediated Coupling of Protein Degradation to Protein Synthesis J. Cell Biol., September 7, 1998; 142(5): 1313 - 1324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Suzuki, T. Shen, M. Poyard, M. Best-Belpomme, J. Hanoune, and N. Defer Expression of adenylyl cyclase mRNAs in the denervated and in the developing mouse skeletal muscle Am J Physiol Cell Physiol, June 1, 1998; 274(6): C1674 - C1685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Weinstein, M. J. Slentz, K. Webster, J. A. Takeuchi, and M. E. Tischler Lysosomal proteolysis in distally or proximally denervated rat soleus muscle Am J Physiol Regulatory Integrative Comp Physiol, October 1, 1997; 273(4): R1562 - R1565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. I. Lewis, T. J. Lorusso, W.-Z. Zhan, and G. C. Sieck Interactive effects of denervation and malnutrition on diaphragm structure and function J Appl Physiol, November 1, 1996; 81(5): 2165 - 2172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Solomon and A. L. Goldberg Importance of the ATP-Ubiquitin-Proteasome Pathway in the Degradation of Soluble and Myofibrillar Proteins in Rabbit Muscle Extracts J. Biol. Chem., October 25, 1996; 271(43): 26690 - 26697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Figueiredo-Pereira, W. E. Chen, J. Li, and O. Johdo The Antitumor Drug Aclacinomycin A, Which Inhibits the Degradation of Ubiquitinated Proteins, Shows Selectivity for the Chymotrypsin-like Activity of the Bovine Pituitary 20S Proteasome J. Biol. Chem., July 12, 1996; 271(28): 16455 - 16459. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |