|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 265, Issue 16, 8986-8988, Jun, 1990
T Restle, B Muller and RS Goody
Recombinant human immunodeficiency virus type 1 reverse transcriptase has
been used to investigate the process of dimer formation and the properties
of the different mono- and dimeric forms of the enzyme. The studies show
that reverse transcriptase activity is exclusively confined to the dimeric
forms. As we also demonstrate, the association rate constant between the
monomers is relatively low so that the dimer- monomer equilibrium is very
slowly established. This offers a new and potentially interesting target
for antiviral chemotherapy with presumably higher specificity than the
currently used nucleoside analogs (Yarchoan, R., Mitsuya, H., Myers, C.E.,
and Broder, S. (1989) N. Eng. J. Med. 321, 726-738), which in their active
triphosphorylated form are also inhibitors of cellular polymerases.
Dimerization of human immunodeficiency virus type 1 reverse transcriptase. A target for chemotherapeutic intervention
Max-Planck-Institut fur Medizinische Forschung, Abteilung Biophysik, Heidelberg, Federal Republic of Germany.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Agopian, E. Gros, G. Aldrian-Herrada, N. Bosquet, P. Clayette, and G. Divita A New Generation of Peptide-based Inhibitors Targeting HIV-1 Reverse Transcriptase Conformational Flexibility J. Biol. Chem., January 2, 2009; 284(1): 254 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Basavapathruni and K. S. Anderson Reverse transcription of the HIV-1 pandemic FASEB J, December 1, 2007; 21(14): 3795 - 3808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Abram and M. A. Parniak Virion Instability of Human Immunodeficiency Virus Type 1 Reverse Transcriptase (RT) Mutated in the Protease Cleavage Site between RT p51 and the RT RNase H Domain J. Virol., September 15, 2005; 79(18): 11952 - 11961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mulky and J. C. Kappes Analysis of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Subunit Structure/Function in the Context of Infectious Virions and Human Target Cells Antimicrob. Agents Chemother., September 1, 2005; 49(9): 3762 - 3769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wapling, K. L. Moore, S. Sonza, J. Mak, and G. Tachedjian Mutations That Abrogate Human Immunodeficiency Virus Type 1 Reverse Transcriptase Dimerization Affect Maturation of the Reverse Transcriptase Heterodimer J. Virol., August 15, 2005; 79(16): 10247 - 10257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. T. Dye, D. J. Miller, and P. Ahlquist In Vivo Self-Interaction of Nodavirus RNA Replicase Protein A Revealed by Fluorescence Resonance Energy Transfer J. Virol., July 15, 2005; 79(14): 8909 - 8919. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Balzarini, J. Auwerx, F. Rodriguez-Barrios, A. Chedad, V. Farkas, F. Ceccherini-Silberstein, C. Garcia-Aparicio, S. Velazquez, E. De Clercq, C.-F. Perno, et al. The Amino Acid Asn136 in HIV-1 Reverse Transcriptase (RT) Maintains Efficient Association of Both RT Subunits and Enables the Rational Design of Novel RT Inhibitors Mol. Pharmacol., July 1, 2005; 68(1): 49 - 60. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Buxton, G. Tachedjian, and J. Mak Analysis of the Contribution of Reverse Transcriptase and Integrase Proteins to Retroviral RNA Dimer Conformation J. Virol., May 15, 2005; 79(10): 6338 - 6348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mulky, S. G. Sarafianos, E. Arnold, X. Wu, and J. C. Kappes Subunit-Specific Analysis of the Human Immunodeficiency Virus Type 1 Reverse Transcriptase In Vivo J. Virol., July 1, 2004; 78(13): 7089 - 7096. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Rothwell, S. Berger, O. Kensch, S. Felekyan, M. Antonik, B. M. Wohrl, T. Restle, R. S. Goody, and C. A. M. Seidel Multiparameter single-molecule fluorescence spectroscopy reveals heterogeneity of HIV-1 reverse transcriptase:primer/template complexes PNAS, February 18, 2003; 100(4): 1655 - 1660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Chaloin, M. J. Lehmann, G. Sczakiel, and T. Restle Endogenous expression of a high-affinity pseudoknot RNA aptamer suppresses replication of HIV-1 Nucleic Acids Res., September 15, 2002; 30(18): 4001 - 4008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Sluis-Cremer, D. Arion, and M. A. Parniak Destabilization of the HIV-1 Reverse Transcriptase Dimer upon Interaction with N-Acyl Hydrazone Inhibitors Mol. Pharmacol., August 1, 2002; 62(2): 398 - 405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Arai, K. Masutomi, S. Khurts, S. Kaneko, K. Kobayashi, and S. Murakami Two Independent Regions of Human Telomerase Reverse Transcriptase Are Important for Its Oligomerization and Telomerase Activity J. Biol. Chem., March 1, 2002; 277(10): 8538 - 8544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Moriarty, S. Huard, S. Dupuis, and C. Autexier Functional Multimerization of Human Telomerase Requires an RNA Interaction Domain in the N Terminus of the Catalytic Subunit Mol. Cell. Biol., February 15, 2002; 22(4): 1253 - 1265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Beattie, W. Zhou, M. O. Robinson, and L. Harrington Functional Multimerization of the Human Telomerase Reverse Transcriptase Mol. Cell. Biol., September 15, 2001; 21(18): 6151 - 6160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Hughes Molecular matchmaking: NNRTIs can enhance the dimerization of HIV type 1 reverse transcriptase PNAS, June 19, 2001; 98(13): 6991 - 6992. [Full Text] [PDF]
|
|
|
|
|
|
G. Tachedjian, H.-E. G. Aronson, and S. P. Goff Analysis of mutations and suppressors affecting interactions between the subunits of the HIV type 1 reverse transcriptase PNAS, June 6, 2000; 97(12): 6334 - 6339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Werner and B. M. Wöhrl Asymmetric Subunit Organization of Heterodimeric Rous Sarcoma Virus Reverse Transcriptase alpha beta : Localization of the Polymerase and RNase H Active Sites in the alpha Subunit J. Virol., April 1, 2000; 74(7): 3245 - 3252. [Abstract] [Full Text]
|
|
|
|
|
|
S. Werner and B. M. Wohrl Soluble Rous Sarcoma Virus Reverse Transcriptases alpha , alpha beta , and beta Purified from Insect Cells Are Processive DNA Polymerases That Lack an RNase H 3' right-arrow 5' Directed Processing Activity J. Biol. Chem., September 10, 1999; 274(37): 26329 - 26336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Morris, V. Robert-Hebmann, L. Chaloin, J. Mery, F. Heitz, C. Devaux, R. S. Goody, and G. Divita A New Potent HIV-1 Reverse Transcriptase Inhibitor. A SYNTHETIC PEPTIDE DERIVED FROM THE INTERFACE SUBUNIT DOMAINS J. Biol. Chem., August 27, 1999; 274(35): 24941 - 24946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Trentin, N. Rebeyrotte, and R. Z. Mamoun Human T-Cell Leukemia Virus Type 1 Reverse Transcriptase (RT) Originates from the pro and pol Open Reading Frames and Requires the Presence of RT-RNase H (RH) and RT-RH-Integrase Proteins for Its Activity J. Virol., August 1, 1998; 72(8): 6504 - 6510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Canard, S. R. Sarfati, and C. C. Richardson Enhanced Binding of Azidothymidine-resistant Human Immunodeficiency Virus 1 Reverse Transcriptase to the 3'-Azido-3'-deoxythymidine 5'-Monophosphate-terminated Primer J. Biol. Chem., June 5, 1998; 273(23): 14596 - 14604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Huang, R. Zensen, M. Cho, and M. A. Martin Construction and Characterization of a Temperature-Sensitive Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant J. Virol., March 1, 1998; 72(3): 2047 - 2054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Wohrl, R. Krebs, S. H. Thrall, S. F. J. Le Grice, A. J. Scheidig, and R. S. Goody Kinetic Analysis of Four HIV-1 Reverse Transcriptase Enzymes Mutated in the Primer Grip Region of p66. IMPLICATIONS FOR DNA SYNTHESIS AND DIMERIZATION J. Biol. Chem., July 11, 1997; 272(28): 17581 - 17587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Divita, J. G. Baillon, K. Rittinger, J.-C. Chermann, and R. S. Goody Interface Peptides as Structure-based Human Immunodeficiency Virus Reverse Transcriptase Inhibitors J. Biol. Chem., December 1, 1995; 270(48): 28642 - 28646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ghosh, K. J. Howard, C. E. Cameron, S. J. Benkovic, S. H. Hughes, and S. F. J. Le Grice Truncating alpha-Helix E` of p66 Human Immunodeficiency Virus Reverse Transcriptase Modulates RNase H Function and Impairs DNA Strand Transfer J. Biol. Chem., March 31, 1995; 270(13): 7068 - 7076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Kensch, B. A. Connolly, H.-J. Steinhoff, A. McGregor, R. S. Goody, and T. Restle HIV-1 Reverse Transcriptase-Pseudoknot RNA Aptamer Interaction Has a Binding Affinity in the Low Picomolar Range Coupled with High Specificity J. Biol. Chem., June 9, 2000; 275(24): 18271 - 18278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Qin, H. Luo, T. Nomura, N. Hayashi, T. Yamashita, and S. Murakami Oligomeric Interaction of Hepatitis C Virus NS5B Is Critical for Catalytic Activity of RNA-dependent RNA Polymerase J. Biol. Chem., January 11, 2002; 277(3): 2132 - 2137. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |