|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 265, Issue 16, 9027-9032, Jun, 1990
M Maeda, K Oshiman, S Tamura and M Futai
The human gastric (H+ + K+)-ATPase gene (15 kilobases) was cloned, and its
nucleotide sequence was determined. The gene has 22 exons and codes a
protein of 1,035 residues including the initiator methionine (Mr =
114,047). A conserved lysine-rich sequence with inserted glycine residues
was found near the amino terminus of the enzyme. The phosphorylation site
and pyridoxal 5'-phosphate- and fluorescein isothiocyanate-binding residues
found in the rat and pig enzymes are also conserved in the human enzyme.
The positions of introns in the human (H+ + K+)-ATPase gene are essentially
the same as those in the human (Na+ + K+)-ATPase alpha and alpha III
subunits; but the first introns of the two enzymes are difficult to align,
and unlike in the (Na+ + K+)-ATPase gene, the sixth exon in the (H+ +
K+)-ATPase gene is not separated by an intron. Furthermore, the ninth
intron is located two bases upstream of the position for the corresponding
intron of the (Na+ + K+)-ATPase alpha III subunit. The similarity in
organization of these two ATPase genes and the homology in the primary
structures of their proteins (approximately 60%) suggest that these two
genes were derived from a common ancestral gene. However, the 5'-flanking
regions of the genes for (H+ + K+)-ATPase and the (Na+ + K+)-ATPase alpha
(+) subunit show no apparent sequence homology, indicating that their
transcriptions are regulated differently. The control region of the
fast-twitch sarcoplasmic reticulum Ca2(+)-ATPase gene also showed no
sequence homology to that of (H+ + K+)-ATPase. The 5'-flanking region of
the (H+ + K+)-ATPase gene contains potential binding sites for RNA
polymerase II and various transcriptional regulation factors and several
direct and inverted repeat sequences which may be important for specific
and controlled expression of the gene in gastric parietal cells. There are
two polyadenylation signals in the 3'-flanking region of the (H+ +
K+)-ATPase gene, but the sequence of this region shows no homology to those
of the corresponding regions of the genes for the (Na+ + K+)-ATPase alpha
and alpha III subunits.
Human gastric (H+ + K+)-ATPase gene. Similarity to (Na+ + K+)-ATPase genes in exon/intron organization but difference in control region
Department of Organic Chemistry and Biochemistry, Osaka University, Japan.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Diller, O. Vagin, G. Sachs, and H.-J. Apell Electrogenic Partial Reactions of the Gastric H,K-ATPase Biophys. J., May 1, 2005; 88(5): 3348 - 3359. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Halleck, R. A. Schlegel, and P. L. Williamson Reanalysis of ATP11B, a Type IV P-type ATPase J. Biol. Chem., March 15, 2002; 277(12): 9736 - 9740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Daiho, K. Yamasaki, H. Suzuki, T. Saino, and T. Kanazawa Deletions or Specific Substitutions of a Few Residues in the NH2-terminal Region (Ala3 to Thr9) of Sarcoplasmic Reticulum Ca2+-ATPase Cause Inactivation and Rapid Degradation of the Enzyme Expressed in COS-1 Cells J. Biol. Chem., August 20, 1999; 274(34): 23910 - 23915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Halleck, D. Pradhan, C. Blackman, C. Berkes, P. Williamson, and R. A. Schlegel Multiple Members of a Third Subfamily of P-Type ATPases Identified by Genomic Sequences and ESTs Genome Res., April 1, 1998; 8(4): 354 - 361. [Abstract] [Full Text]
|
|
|
|
|
|
T. Saino, T. Daiho, and T. Kanazawa Modification of Arginine-198 in Sarcoplasmic Reticulum Ca2+-ATPase by 1,2-Cyclohexanedione Causes Inhibition of Formation of the Phosphoenzyme Intermediate from Inorganic Phosphate J. Biol. Chem., August 22, 1997; 272(34): 21142 - 21150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Asano, S. Matsuda, Y. Tega, K. Shimizu, S. Sakamoto, and N. Takeguchi Mutational Analysis of Putative SCH 28080 Binding Sites of the Gastric H+,K+-ATPase J. Biol. Chem., July 11, 1997; 272(28): 17668 - 17674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kimura, H. Suzuki, T. Daiho, K. Yamasaki, and T. Kanazawa Identification of Arginyl Residues Located at the ATP Binding Site of Sarcoplasmic Reticulum Ca2+-ATPase. MODIFICATION WITH 1,2-CYCLOHEXANEDIONE J. Biol. Chem., November 15, 1996; 271(46): 28933 - 28941. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Asano, Y. Tega, K. Konishi, M. Fujioka, and N. Takeguchi Functional Expression of Gastric H[IMAGE],K[IMAGE]-ATPase and Site-directed Mutagenesis of the Putative Cation Binding Site and the Catalytic Center J. Biol. Chem., February 2, 1996; 271(5): 2740 - 2745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Togawa, T. Ishiguro, S. Kaya, A. Shimada, T. Imagawa, and K. Taniguchi Reversible Phosphorylation of Both Tyr[IMAGE] and Tyr[IMAGE] in the [IMAGE]-Chain of Pig Stomach H[IMAGE],K[IMAGE]-ATPase by a Membrane-bound Kinase and a Phosphatase J. Biol. Chem., June 30, 1995; 270(26): 15475 - 15478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Daiho, K. Yamasaki, T. Saino, M. Kamidochi, K. Satoh, H. Iizuka, and H. Suzuki Mutations of Either or Both Cys876 and Cys888 Residues of Sarcoplasmic Reticulum Ca2+-ATPase Result in a Complete Loss of Ca2+ Transport Activity without a Loss of Ca2+-dependent ATPase Activity. ROLE OF THE CYS876-CYS888 DISULFIDE BOND J. Biol. Chem., August 24, 2001; 276(35): 32771 - 32778. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |