|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 265, Issue 17, 10073-10080, Jun, 1990
YN Chen, LA Mickley, AM Schwartz, EM Acton, JL Hwang and AT Fojo
Development of multidrug resistance due to overexpression of P-
glycoprotein (Pgp), a cell membrane drug efflux pump, occurs commonly
during in vitro selections with adriamycin (Adr). Pgp-mediated drug
resistance can be overcome by the calcium channel blocker verapamil (Vp),
which acts as a competitive inhibitor of drug binding and efflux. In order
to identify other mechanisms of Adr resistance, we isolated an
Adr-resistant subline by selecting the human breast cancer cell line MCF-7
with incremental increases of Adr in the presence of 10 microgram/ml
verapamil. The resultant MCF-7/AdrVp subline is 900-fold resistant to Adr,
does not overexpress Pgp, and does not exhibit a decrease in Adr
accumulation. It exhibits a unique cross-resistance pattern: high
cross-resistance to the potent Adr analogue 3'-deamino-3'-
(3-cyano-4-morpholinyl)doxorubicin, lower cross-resistance to the
alkylating agent melphalan, and a sensitivity similar to the parental cell
line to vinblastine. The levels of glutathione and glutathione S-
transferase are similar in the parental line and the Adr-resistant subline.
Topoisomerase II-DNA complexes measured by the potassium- sodium dodecyl
sulfate precipitation method shows a 2-3 fold decrease in the resistant
subline. The MCF-7/AdrVp cells overexpress a novel membrane protein with an
apparent molecular mass of 95 kDa. Polyclonal antibodies raised against the
P-95 protein demonstrate a correaltion between the level of expression and
Adr resistance. Removal of Adr but not verapamil from the selection media
results in a decline in P-95 protein levels that parallels a restoration of
sensitivity to Adr. Immunohistochemistry demonstrates localization of the
P-95 protein on the cell surface. The demonstration of high levels of the
protein in clinical samples obtained from patients refractory to Adr
suggests that this protein may play a role in clinical drug resistance.
Characterization of adriamycin-resistant human breast cancer cells which display overexpression of a novel resistance-related membrane protein
Medicine Branch, National Cancer Institute, Bethesda, Maryland 20892.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Liu, Y. Liu, and J.-T. Zhang A new mechanism of drug resistance in breast cancer cells: fatty acid synthase overexpression-mediated palmitate overproduction Mol. Cancer Ther., February 1, 2008; 7(2): 263 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-P. Wu, S. Shukla, A. M. Calcagno, M. D. Hall, M. M. Gottesman, and S. V. Ambudkar Evidence for dual mode of action of a thiosemicarbazone, NSC73306: a potent substrate of the multidrug resistance linked ABCG2 transporter Mol. Cancer Ther., December 1, 2007; 6(12): 3287 - 3296. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Hatle, W. Neveu, O. Dienz, S. Rymarchyk, R. Barrantes, S. Hale, N. Farley, K. M. Lounsbury, J. P. Bond, D. Taatjes, et al. Methylation-Controlled J Protein Promotes c-Jun Degradation To Prevent ABCB1 Transporter Expression Mol. Cell. Biol., April 15, 2007; 27(8): 2952 - 2966. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Takahashi, T. Furuchi, and A. Naganuma Endocytic Ark/Prk Kinases Play a Critical Role in Adriamycin Resistance in Both Yeast and Mammalian Cells Cancer Res., December 15, 2006; 66(24): 11932 - 11937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Seamon, C. A. Rugg, S. Emanuel, A. M. Calcagno, S. V. Ambudkar, S. A. Middleton, J. Butler, V. Borowski, and L. M. Greenberger Role of the ABCG2 drug transporter in the resistance and oral bioavailability of a potent cyclin-dependent kinase/Aurora kinase inhibitor. Mol. Cancer Ther., October 1, 2006; 5(10): 2459 - 2467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Chearwae, S. Shukla, P. Limtrakul, and S. V. Ambudkar Modulation of the function of the multidrug resistance-linked ATP-binding cassette transporter ABCG2 by the cancer chemopreventive agent curcumin. Mol. Cancer Ther., August 1, 2006; 5(8): 1995 - 2006. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nakanishi, K. Shiozawa, B. A. Hassel, and D. D. Ross Complex interaction of BCRP/ABCG2 and imatinib in BCR-ABL-expressing cells: BCRP-mediated resistance to imatinib is attenuated by imatinib-induced reduction of BCRP expression Blood, July 15, 2006; 108(2): 678 - 684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu, H. Liu, B. Han, and J.-T. Zhang Identification of 14-3-3{sigma} as a Contributor to Drug Resistance in Human Breast Cancer Cells Using Functional Proteomic Analysis. Cancer Res., March 15, 2006; 66(6): 3248 - 3255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Shafran, I. Ifergan, E. Bram, G. Jansen, I. Kathmann, G. J. Peters, R. W. Robey, S. E. Bates, and Y. G. Assaraf ABCG2 Harboring the Gly482 Mutation Confers High-Level Resistance to Various Hydrophilic Antifolates Cancer Res., September 15, 2005; 65(18): 8414 - 8422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Behbod and J. M. Rosen Will cancer stem cells provide new therapeutic targets? Carcinogenesis, April 1, 2005; 26(4): 703 - 711. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Zhao and X.-b. Chang Mutation of the Aromatic Amino Acid Interacting with Adenine Moiety of ATP to a Polar Residue Alters the Properties of Multidrug Resistance Protein 1 J. Biol. Chem., November 19, 2004; 279(47): 48505 - 48512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Y. Chen, S.-J. Shih, M. Hsiao, M. L. Rothenberg, and M. Prudhomme Induction of Radiosensitization by Indolocarbazole Derivatives: The Role of DNA Topoisomerase I Mol. Pharmacol., September 1, 2004; 66(3): 553 - 560. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Bates, W. Y. Medina-Perez, G. Kohlhagen, S. Antony, T. Nadjem, R. W. Robey, and Y. Pommier ABCG2 Mediates Differential Resistance to SN-38 (7-Ethyl-10-hydroxycamptothecin) and Homocamptothecins J. Pharmacol. Exp. Ther., August 1, 2004; 310(2): 836 - 842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Piekarz, R. W. Robey, Z. Zhan, G. Kayastha, A. Sayah, A. H. Abdeldaim, S. Torrico, and S. E. Bates T-cell lymphoma as a model for the use of histone deacetylase inhibitors in cancer therapy: impact of depsipeptide on molecular markers, therapeutic targets, and mechanisms of resistance Blood, June 15, 2004; 103(12): 4636 - 4643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakanishi, L. A. Doyle, B. Hassel, Y. Wei, K. S. Bauer, S. Wu, D. W. Pumplin, H.-B. Fang, and D. D. Ross Functional Characterization of Human Breast Cancer Resistance Protein (BCRP, ABCG2) Expressed in the Oocytes of Xenopus laevis Mol. Pharmacol., December 1, 2003; 64(6): 1452 - 1462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sabourin, J. L. Nitiss, K. C. Nitiss, K. Tatebayashi, H. Ikeda, and N. Osheroff Yeast recombination pathways triggered by topoisomerase II-mediated DNA breaks Nucleic Acids Res., August 1, 2003; 31(15): 4373 - 4384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Volk, K. M. Farley, Y. Wu, F. Li, R. W. Robey, and E. Schneider Overexpression of Wild-Type Breast Cancer Resistance Protein Mediates Methotrexate Resistance Cancer Res., September 1, 2002; 62(17): 5035 - 5040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Allen, S. C. Jackson, and A. H. Schinkel A Mutation Hot Spot in the Bcrp1 (Abcg2) Multidrug Transporter in Mouse Cell Lines Selected for Doxorubicin Resistance Cancer Res., April 1, 2002; 62(8): 2294 - 2299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Allen and A. H. Schinkel Multidrug Resistance and Pharmacological Protection Mediated by the Breast Cancer Resistance Protein (BCRP/ABCG2) Mol. Cancer Ther., April 1, 2002; 1(6): 427 - 434. [Full Text] [PDF]
|
|
|
|
 |
|
 I. F. Faneyte, P. M. P. Kristel, M. Maliepaard, G. L. Scheffer, R. J. Scheper, J. H. M. Schellens, and M. J. van de Vijver Expression of the Breast Cancer Resistance Protein in Breast Cancer Clin. Cancer Res., April 1, 2002; 8(4): 1068 - 1074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. Robey, W. Y. Medina-Pérez, K. Nishiyama, T. Lahusen, K. Miyake, T. Litman, A. M. Senderowicz, D. D. Ross, and S. E. Bates Overexpression of the ATP-binding Cassette Half-Transporter, ABCG2 (MXR/BCRP/ABCP1), in Flavopiridol-resistant Human Breast Cancer Cells Clin. Cancer Res., January 1, 2001; 7(1): 145 - 152. [Abstract] [Full Text]
|
|
|
|
 |
|
 T Litman, M Brangi, E Hudson, P Fetsch, A Abati, D. Ross, K Miyake, J. Resau, and S. Bates The multidrug-resistant phenotype associated with overexpression of the new ABC half-transporter, MXR (ABCG2) J. Cell Sci., January 6, 2000; 113(11): 2011 - 2021. [Abstract] [PDF]
|
|
|
|
|
|
S. K. Rabindran, D. D. Ross, L. A. Doyle, W. Yang, and L. M. Greenberger Fumitremorgin C Reverses Multidrug Resistance in Cells Transfected with the Breast Cancer Resistance Protein Cancer Res., January 1, 2000; 60(1): 47 - 50. [Abstract] [Full Text]
|
|
|
|
|
|
J. D. Allen, R. F. Brinkhuis, J. Wijnholds, and A. H. Schinkel The Mouse Bcrp1/Mxr/Abcp Gene: Amplification and Overexpression in Cell Lines Selected for Resistance to Topotecan, Mitoxantrone, or Doxorubicin Cancer Res., September 1, 1999; 59(17): 4237 - 4241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. McAleer, M. A. Breen, N. L. White, and N. Matthews pABC11 (Also Known as MOAT-C and MRP5), a Member of the ABC Family of Proteins, Has Anion Transporter Activity but Does Not Confer Multidrug Resistance When Overexpressed in Human Embryonic Kidney 293 Cells J. Biol. Chem., August 13, 1999; 274(33): 23541 - 23548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Ross, W. Yang, L. V. Abruzzo, W. S. Dalton, E. Schneider, H. Lage, M. Dietel, L. Greenberger, S. P. C. Cole, and L. A. Doyle Atypical Multidrug Resistance: Breast Cancer Resistance Protein Messenger RNA Expression in Mitoxantrone-Selected Cell Lines J Natl Cancer Inst, March 3, 1999; 91(5): 429 - 433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Advani, H. I. Saba, M. S. Tallman, J. M. Rowe, P. H. Wiernik, J. Ramek, K. Dugan, B. Lum, J. Villena, E. Davis, et al. Treatment of Refractory and Relapsed Acute Myelogenous Leukemia With Combination Chemotherapy Plus the Multidrug Resistance Modulator PSC 833 (Valspodar) Blood, February 1, 1999; 93(3): 787 - 795. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Doyle, W. Yang, L. V. Abruzzo, T. Krogmann, Y. Gao, A. K. Rishi, and D. D. Ross A multidrug resistance transporter from human MCF-7 breast cancer cells PNAS, December 22, 1998; 95(26): 15665 - 15670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Giannakakou, D. L. Sackett, Y.-K. Kang, Z. Zhan, J. T. M. Buters, T. Fojo, and M. S. Poruchynsky Paclitaxel-resistant Human Ovarian Cancer Cells Have Mutant beta -Tubulins That Exhibit Impaired Paclitaxel-driven Polymerization J. Biol. Chem., July 4, 1997; 272(27): 17118 - 17125. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |