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J. Biol. Chem., Vol. 265, Issue 17, 10164-10171, 06, 1990
JE Lindsley and MM Cox
The recA protein from Escherichia coli can homologously align two duplex
DNA molecules; however, this interaction is much less efficient than the
alignment of a single strand and a duplex. Three strand paranemic joints
are readily detected. In contrast, duplex-duplex pairing is detected only
when the incoming (second) duplex is negatively supercoiled, and even here
the pairing is inefficient. The recA protein-promoted four strand exchange
reaction is initiated in a three strand region, with efficiency increasing
with the length of potential three strand pairing available for initiation.
This indicates that a paranemic joint involving three DNA strands may be an
important intermediate in all recA protein-mediated DNA strand exchange
reactions and that the presence of three strands rather than four is a
fundamental structural parameter of paranemic joints.
On RecA protein-mediated homologous alignment of two DNA molecules. Three strands versus four strands
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706.
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  N. Haruta, X. Yu, S. Yang, E. H. Egelman, and M. M. Cox A DNA Pairing-enhanced Conformation of Bacterial RecA Proteins J. Biol. Chem., December 26, 2003; 278(52): 52710 - 52723. [Abstract] [Full Text] [PDF]
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J. M. Bork, M. M. Cox, and R. B. Inman RecA Protein Filaments Disassemble in the 5' to 3' Direction on Single-stranded DNA J. Biol. Chem., November 30, 2001; 276(49): 45740 - 45743. [Abstract] [Full Text] [PDF]
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 E. N. Zaitsev and S. C. Kowalczykowski A novel pairing process promoted by Escherichia coli RecA protein: inverse DNA and RNA strand exchange Genes & Dev., March 15, 2000; 14(6): 740 - 749. [Abstract] [Full Text]
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B. L. Webb, M. M. Cox, and R. B. Inman ATP Hydrolysis and DNA Binding by the Escherichia coli RecF Protein J. Biol. Chem., May 28, 1999; 274(22): 15367 - 15374. [Abstract] [Full Text] [PDF]
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B. C. Wong, S.-K. Chiu, and S. A. Chow The Role of Negative Superhelicity and Length of Homology in the Formation of Paranemic Joints Promoted by RecA Protein J. Biol. Chem., May 15, 1998; 273(20): 12120 - 12127. [Abstract] [Full Text] [PDF]
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K. J. MacFarland, Q. Shan, R. B. Inman, and M. M. Cox RecA as a Motor Protein. TESTING MODELS FOR THE ROLE OF ATP HYDROLYSIS IN DNA STRAND EXCHANGE J. Biol. Chem., July 11, 1997; 272(28): 17675 - 17685. [Abstract] [Full Text] [PDF]
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Q. Shan, M. M. Cox, and R. B. Inman DNA Strand Exchange Promoted by RecA K72R J. Biol. Chem., March 8, 1996; 271(10): 5712 - 5724. [Abstract] [Full Text] [PDF]
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W. A. Bedale and M. M. Cox Evidence for the Coupling of ATP Hydrolysis to the Final (Extension) Phase of RecA Protein-mediated DNA Strand Exchange J. Biol. Chem., March 8, 1996; 271(10): 5725 - 5732. [Abstract] [Full Text] [PDF]
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M. M. Cox and M. M. Cox Alignment of 3 (but Not 4) DNA Strands within a RecA Protein Filament J. Biol. Chem., November 3, 1995; 270(44): 26021 - 26024. [Full Text] [PDF]
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