HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Felipo, V. Articles by Grisolia, S. Search for Related Content PubMed PubMed Citation Articles by Felipo, V. Articles by Grisolia, S. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 265, Issue 17, 9599-9601, Jun, 1990

A specific inhibitor of protein kinase C induces differentiation of neuroblastoma cells

V Felipo, MD Minana and S Grisolia
Instituto de Investigaciones Citologicas de la Caja de Ahorros de Valencia, Centro Asociado del Consejo Superior de Investigaciones Cientificas, Spain.

Recent reports suggest that protein kinase C is involved in neural differentiation. We show that 1-(5-isoquinolinylsulfonyl)-2- methylpiperazine (H7), the more specific inhibitor of protein kinase C known, induces morphological and functional differentiation of neuro 2a cells, as indicated by the marked increase in the number of neurites/cell and in acetylcholinesterase activity. HA 1004 does not induce differentiation of neural cells. The induction of differentiation by H7 was very rapid; 3 h after addition of H7 the percentages of differentiated cells were 17, 33, 37, 55, and 75% for 17, 50, 85, 250, and 500 microM H7, respectively, while for controls it was 9%. When 500 microM H7 was added to the culture medium, protein kinase C was inhibited by 72 and 62% in cytosol and membrane, respectively. Also, acetylcholinesterase activity (a marker of functional differentiation) increased with time, reaching a 7-fold increase after 48 h.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. Ronca, S.-L. Chan, and V. C. Yu 1-(5-Isoquinolinesulfonyl)-2-methylpiperazine Induces Apoptosis in Human Neuroblastoma Cells, SH-SY5Y, through a p53-dependent Pathway J. Biol. Chem., February 14, 1997; 272(7): 4252 - 4260. [Abstract] [Full Text] [PDF]

				M. J. Clemens, I. Trayner, and J. Menaya The role of protein kinase C isoenzymes in the regulation of cell proliferation and differentiation J. Cell Sci., December 1, 1992; 103(4): 881 - 887. [PDF]