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J. Biol. Chem., Vol. 265, Issue 18, 10196-10197, 06, 1990

Crystallization and preliminary x-ray investigation of colicin E3 in complex with its immunity protein

F Frolow and M Shoham
Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.

Crystals of the colicin E3-immunity protein complex have been grown from solutions of citrate at pH 5.6. The crystals are monoclinic, space group P2(1), with unit cell dimensions a = 67.71, b = 196.67, c = 85.58 A, and beta = 113.67 degrees. The crystals diffract to 3-A resolution and are stable in the x-ray beam for at least a day. Although the stoichiometry of the complex in solution is 1:1 there are two, three, or four such binary complex molecules in the asymmetric unit.
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