HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hammarback, J. A. Articles by Vallee, R. B. Search for Related Content PubMed PubMed Citation Articles by Hammarback, J. A. Articles by Vallee, R. B. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 265, Issue 22, 12763-12766, Aug, 1990

Antibody exchange immunochemistry

JA Hammarback and RB Vallee
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.

Antibodies are found to transfer rapidly between antigen samples attached to separate solid supports. The half-times for this antibody exchange at 20 and 37 degrees C were 7 and 1 h, respectively. Antibody exchange was exhibited by all 17 polyclonal and monoclonal antibodies tested and was readily detected by enzyme-linked immunosorbent assay, immunoblotting, or immunocytochemical assays. Taking advantage of this phenomenon, antibodies can be affinity-purified using less than a picomole of antigen and small amounts of antibody.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. Allen, N. Bala, R. Ali, D. Nishida, M. Aihara, M Ishida, and A. Fok Rapid bulk replacement of acceptor membrane by donor membrane during phagosome to phagoacidosome transformation in Paramecium J. Cell Sci., January 3, 1995; 108(3): 1263 - 1274. [Abstract] [PDF]