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J. Biol. Chem., Vol. 265, Issue 22, 12771-12773, 08, 1990
JC Boyington, BJ Gaffney and LM Amzel
Crystals of lipoxygenase-1 from soybeans have been grown by the method of
vapor diffusion in the presence of sodium formate, ammonium acetate, and
lithium chloride at pH 7.0. This enzyme contains a non-heme iron and is
closely related to a human lipoxygenase found in leukocytes that
participates in the biosynthesis of leukotrienes and lipoxins. The crystals
are monoclinic space group C2 with cell dimensions of a = 183.8 A, b =
123.2 A, c = 94.3 A and beta = 102.9 degrees. They diffract beyond 2.7 A,
are stable for several days in the x-ray beam, and appear to be suitable
for x-ray diffraction studies.
Crystallization and preliminary x-ray analysis of soybean lipoxygenase- 1, a non-heme iron-containing dioxygenase
Department of Biophysics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
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