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J. Biol. Chem., Vol. 265, Issue 22, 12771-12773, 08, 1990

Crystallization and preliminary x-ray analysis of soybean lipoxygenase- 1, a non-heme iron-containing dioxygenase

JC Boyington, BJ Gaffney and LM Amzel
Department of Biophysics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

Crystals of lipoxygenase-1 from soybeans have been grown by the method of vapor diffusion in the presence of sodium formate, ammonium acetate, and lithium chloride at pH 7.0. This enzyme contains a non-heme iron and is closely related to a human lipoxygenase found in leukocytes that participates in the biosynthesis of leukotrienes and lipoxins. The crystals are monoclinic space group C2 with cell dimensions of a = 183.8 A, b = 123.2 A, c = 94.3 A and beta = 102.9 degrees. They diffract beyond 2.7 A, are stable for several days in the x-ray beam, and appear to be suitable for x-ray diffraction studies.
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This article has been cited by other articles:


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M. L. Oldham, A. R. Brash, and M. E. Newcomer
Insights from the X-ray Crystal Structure of Coral 8R-Lipoxygenase: CALCIUM ACTIVATION VIA A C2-LIKE DOMAIN AND A STRUCTURAL BASIS OF PRODUCT CHIRALITY
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J. Boyington, B. Gaffney, and L. Amzel
The three-dimensional structure of an arachidonic acid 15-lipoxygenase
Science, June 4, 1993; 260(5113): 1482 - 1486.
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