| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 265, Issue 22, 12880-12886, Aug, 1990
U Saxena, MG Klein and IJ Goldberg
Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
Lipoprotein lipase (LPL) hydrolyzes triglyceride in plasma lipoprotein primarily while bound to vascular endothelial cells. LPL metabolism by cultured endothelial cells was studied. Purified radioiodinated bovine LPL bound to porcine aortic endothelial cells at 4 degrees C with an association constant of 0.18 x 10(7) m-1. Analysis of the time course of LPL dissociation from endothelial cells at 4 degrees C yielded a dissociation rate constant of 3.9 x 10(-6)s-1. After 1 h at 37 degrees C, 28% of the LPL initially bound to the cell surface was no longer releasable by heparin or trypsin treatments, suggesting that LPL was internalized by the cells. Addition of heparin to the medium or pretreatment of the cells with heparinase markedly reduced the amount of LPL internalized, establishing a requirement for cell surface heparan sulfate proteoglycans in the process. When cells containing internalized LPL were incubated at 37 degrees C, a time-dependent increase in the amount of LPL in the medium and a corresponding decrease in LPL associated with the cells was found. This suggested that internalized LPL was released back into the medium. The catalytic activity, molecular size, and heparin-binding characteristics of the released LPL was similar to native LPL. Addition of either heparin, heparinase, or excess unlabeled LPL to prevent the rebinding of released 125I-LPL to the cell surface increased the amount of 125I-LPL present in the medium, suggesting that there is a process of recycling of 125I-LPL bound to the cell surface. Studies examining the effect of pH on dissociation of LPL from its binding site showed less dissociation of cell surface bound LPL at pH 5.5 compared with pH 7.4 and 8.5. These results suggest that even at acidic pH as in endocytotic vesicles, LPL remains bound to proteoglycans and this may facilitate the recycling of internalized LPL molecules.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  G. Qiu and J. S. Hill Endothelial lipase enhances low density lipoprotein binding and cell association in THP-1 macrophages Cardiovasc Res, December 1, 2007; 76(3): 528 - 538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Qi, K.-H. Kuo, A. Abrahani, D. An, Y. Qi, J. Heung, G. Kewalramani, T. Pulinilkunnil, S. Ghosh, S. M. Innis, et al. Acute intralipid infusion reduces cardiac luminal lipoprotein lipase but recruits additional enzyme from cardiomyocytes Cardiovasc Res, October 1, 2006; 72(1): 124 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Spillmann, A. Lookene, and G. Olivecrona Isolation and Characterization of Low Sulfated Heparan Sulfate Sequences with Affinity for Lipoprotein Lipase J. Biol. Chem., August 18, 2006; 281(33): 23405 - 23413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rip, M. C. Nierman, N. J. Wareham, R. Luben, S. A. Bingham, N. E. Day, J. N.I. van Miert, B. A. Hutten, J. J.P. Kastelein, J. A. Kuivenhoven, et al. Serum Lipoprotein Lipase Concentration and Risk for Future Coronary Artery Disease: The EPIC-Norfolk Prospective Population Study Arterioscler. Thromb. Vasc. Biol., March 1, 2006; 26(3): 637 - 642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Pulinilkunnil and B. Rodrigues Cardiac lipoprotein lipase: Metabolic basis for diabetic heart disease Cardiovasc Res, February 1, 2006; 69(2): 329 - 340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. R. Hegde, C. Dunn, D. M. Lewinsohn, M. A. Jarvis, J. A. Nelson, and D. C. Johnson Endogenous human cytomegalovirus gB is presented efficiently by MHC class II molecules to CD4+ CTL J. Exp. Med., October 17, 2005; 202(8): 1109 - 1119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Koike, J. Liang, X. Wang, T. Ichikawa, M. Shiomi, G. Liu, H. Sun, S. Kitajima, M. Morimoto, T. Watanabe, et al. Overexpression of Lipoprotein Lipase in Transgenic Watanabe Heritable Hyperlipidemic Rabbits Improves Hyperlipidemia and Obesity J. Biol. Chem., February 27, 2004; 279(9): 7521 - 7529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sambandam, M. A. Abrahani, S. Craig, O. Al-Atar, E. Jeon, and B. Rodrigues Metabolism of VLDL is increased in streptozotocin-induced diabetic rat hearts Am J Physiol Heart Circ Physiol, June 1, 2000; 278(6): H1874 - H1882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Obunike, S. Pillarisetti, L. Paka, Y. Kako, M. J. Butteri, Y.-Y. Ho, W. D. Wagner, N. Yamada, T. Mazzone, R. J. Deckelbaum, et al. The Heparin-Binding Proteins Apolipoprotein E and Lipoprotein Lipase Enhance Cellular Proteoglycan Production Arterioscler. Thromb. Vasc. Biol., January 1, 2000; 20(1): 111 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Sambandam, M. A. Abrahani, E. St. Pierre, O. Al-Atar, M. C. Cam, and B. Rodrigues Localization of Lipoprotein Lipase in the Diabetic Heart : Regulation by Acute Changes in Insulin Arterioscler. Thromb. Vasc. Biol., June 1, 1999; 19(6): 1526 - 1534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Marino, G. Zheng, and R. T. McCluskey Megalin (gp330) Is an Endocytic Receptor for Thyroglobulin on Cultured Fisher Rat Thyroid Cells J. Biol. Chem., April 30, 1999; 274(18): 12898 - 12904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. U. Huey, T. Marcell, G. C. Owens, J. Etienne, and R. H. Eckel Lipoprotein lipase is expressed in cultured Schwann cells and functions in lipid synthesis and utilization J. Lipid Res., November 1, 1998; 39(11): 2135 - 2142. [Abstract] [Full Text]
|
|
|
|
|
|
J. W. Burgess, D. R. Gould, and Y. L. Marcel The HepG2 Extracellular Matrix Contains Separate Heparinase- and Lipid-releasable Pools of ApoE. IMPLICATIONS FOR HEPATIC LIPOPROTEIN METABOLISM J. Biol. Chem., March 6, 1998; 273(10): 5645 - 5654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Shepherd, M. C. Cam, N. Sambandam, M. A. Abrahani, and B. Rodrigues Streptozotocin-Induced Diabetes Enhances Cardiac Heparin-Releasable Lipoprotein Lipase Activity in Spontaneously Hypertensive Rats Hypertension, March 1, 1998; 31(3): 878 - 884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Summerford and R. J. Samulski Membrane-Associated Heparan Sulfate Proteoglycan Is a Receptor for Adeno-Associated Virus Type 2 Virions J. Virol., February 1, 1998; 72(2): 1438 - 1445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ho, G. J. Broze Jr., and A. L. Schwartz Role of Heparan Sulfate Proteoglycans in the Uptake and Degradation of Tissue Factor Pathway Inhibitor-Coagulation Factor Xa Complexes J. Biol. Chem., July 4, 1997; 272(27): 16838 - 16844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tumova and K. J. Bame The Interaction between Basic Fibroblast Growth Factor and Heparan Sulfate Can Prevent the in Vitro Degradation of the Glycosaminoglycan by Chinese Hamster Ovary Cell Heparanases J. Biol. Chem., April 4, 1997; 272(14): 9078 - 9085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-L. Shyng, S. Lehmann, K. L. Moulder, and D. A. Harris Sulfated Glycans Stimulate Endocytosis of the Cellular Isoform of the Prion Protein, PrP^C, in Cultured Cells J. Biol. Chem., December 15, 1995; 270(50): 30221 - 30229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Argraves, F. D. Battey, C. D. MacCalman, K. R. McCrae, M. Gåfvels, K. F. Kozarsky, D. A. Chappell, J. F. Strauss , III, and D. K. Strickland The Very Low Density Lipoprotein Receptor Mediates the Cellular Catabolism of Lipoprotein Lipase and Urokinase-Plasminogen Activator Inhibitor Type I Complexes J. Biol. Chem., November 3, 1995; 270(44): 26550 - 26557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Berryman and A. Bensadoun Heparan Sulfate Proteoglycans Are Primarily Responsible for the Maintenance of Enzyme Activity, Binding, and Degradation of Lipoprotein Lipase in Chinese Hamster Ovary Cells J. Biol. Chem., October 13, 1995; 270(41): 24525 - 24531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shimada, S. Ishibashi, T. Gotoda, M. Kawamura, K. Yamamoto, T. Inaba, K. Harada, J. Ohsuga, S. Perrey, Y. Yazaki, et al. Overexpression of Human Lipoprotein Lipase Protects Diabetic Transgenic Mice From Diabetic Hypertriglyceridemia and Hypercholesterolemia Arterioscler. Thromb. Vasc. Biol., October 1, 1995; 15(10): 1688 - 1694. [Abstract] [Full Text]
|
|
|
|
|
|
P. Tornvall, G. Olivecrona, F. Karpe, A. Hamsten, and T. Olivecrona Lipoprotein Lipase Mass and Activity in Plasma and Their Increase After Heparin Are Separate Parameters With Different Relations to Plasma Lipoproteins Arterioscler. Thromb. Vasc. Biol., August 1, 1995; 15(8): 1086 - 1093. [Abstract] [Full Text]
|
|
|
|
 |
|
 N Quarto and F Amalric Heparan sulfate proteoglycans as transducers of FGF-2 signalling J. Cell Sci., January 11, 1994; 107(11): 3201 - 3212. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Anderson, B. Kamen, K. Rothberg, and S. Lacey Potocytosis: sequestration and transport of small molecules by caveolae Science, January 24, 1992; 255(5043): 410 - 411. [PDF]
|
|
|
|
|
|
J. C. Obunike, E. P. Lutz, Z. Li, L. Paka, T. Katopodis, D. K. Strickland, K. F. Kozarsky, S. Pillarisetti, and I. J. Goldberg Transcytosis of Lipoprotein Lipase across Cultured Endothelial Cells Requires Both Heparan Sulfate Proteoglycans and the Very Low Density Lipoprotein Receptor J. Biol. Chem., March 16, 2001; 276(12): 8934 - 8941. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
