| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 265, Issue 23, 13566-13571, 08, 1990
LA Eichacker, J Soll, P Lauterbach, W Rudiger, RR Klein and JE Mullet
An in vitro translation system using lysed etioplasts was developed to test
if the accumulation of plastid-encoded chlorophyll a apoproteins is
dependent on the de novo synthesis of chlorophyll a. The P700 apoproteins,
CP47 and CP43, were not radiolabeled in pulsechase translation assays
employing lysed etioplasts in the absence of added chlorophyll precursors.
When chlorophyllide a plus phytylpyrophosphate were added to lysed
etioplast translation assays in the dark, chlorophyll a was synthesized and
radiolabeled P700 apoproteins, CP47 and CP43, and a protein which
comigrates with D1 accumulated. Chlorophyllide a or phytylpyrophosphate
added separately to the translation assay in darkness did not induce
chlorophyll a formation or chlorophyll a apoprotein accumulation.
Chlorophyll a formation and chlorophyll a apoprotein accumulation were also
induced in the lysed etioplast translation system by the photoreduction of
protochlorophyllide to chlorophyllide a in the presence of exogenous
phytylpyrophosphate. Accumulation of radiolabeled CP47 was detectable when
very low levels of chlorophyll a were synthesized de novo (less than 0.01
nmol/10(7) plastids), and radiolabel increased linearly with increasing de
novo chlorophyll a formation. Higher levels of de novo synthesized
chlorophyll a were required prior to detection of radiolabel incorporation
into the P700 apoproteins and CP43 (greater than 0.01 nmol/10(7) plastids).
Radiolabel incorporation into the P700 apoproteins, CP47 and CP43,
saturated at a chlorophyll a concentration which corresponds to 50% of the
etioplast protochlorophyllide content (0.06 nmol of chlorophyll a/10(7)
plastids).
In vitro synthesis of chlorophyll a in the dark triggers accumulation of chlorophyll a apoproteins in barley etioplasts
Department of Botany, University of Munich, Federal Republic of Germany.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Wu, X. Zhang, B. He, L. Diao, S. Sheng, J. Wang, X. Guo, N. Su, L. Wang, L. Jiang, et al. A Chlorophyll-Deficient Rice Mutant with Impaired Chlorophyllide Esterification in Chlorophyll Biosynthesis Plant Physiology, September 1, 2007; 145(1): 29 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Amann, L. Lezhneva, G. Wanner, R. G. Herrmann, and J. Meurer ACCUMULATION OF PHOTOSYSTEM ONE1, a Member of a Novel Gene Family, Is Required for Accumulation of [4Fe-4S] Cluster-Containing Chloroplast Complexes and Antenna Proteins PLANT CELL, November 1, 2004; 16(11): 3084 - 3097. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kim and J. E. Mullet A Mechanism for Light-Induced Translation of the rbcL mRNA Encoding the Large Subunit of Ribulose-1,5-bisphosphate Carboxylase in Barley Chloroplasts Plant Cell Physiol., May 15, 2003; 44(5): 491 - 499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Matsuo and J. Obokata Dual Roles of Photosynthetic Electron Transport in Photosystem I Biogenesis: Light Induction of mRNAs and Chromatic Regulation at Post-mRNA Level Plant Cell Physiol., October 15, 2002; 43(10): 1189 - 1197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Müller and L. A. Eichacker Assembly of the D1 Precursor in Monomeric Photosystem II Reaction Center Precomplexes Precedes Chlorophyll a-Triggered Accumulation of Reaction Center II in Barley Etioplasts PLANT CELL, December 1, 1999; 11(12): 2365 - 2378. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Kuttkat, I. Edhofer, L. A. Eichacker, and H. Paulsen Light-harvesting Chlorophyll a/b-binding Protein Stably Inserts into Etioplast Membranes Supplemented with Zn-pheophytin a/b J. Biol. Chem., August 15, 1997; 272(33): 20451 - 20455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Oster, C. E. Bauer, and W. Rudiger Characterization of Chlorophyll a and Bacteriochlorophyll a Synthases by Heterologous Expression in Escherichia coli J. Biol. Chem., April 11, 1997; 272(15): 9671 - 9676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Eichacker, M. Helfrich, W. Rudiger, and B. Muller Stabilization of Chlorophyll a-binding Apoproteins P700, CP47, CP43, D2, and D1 by Chlorophyll a or Zn-pheophytin a J. Biol. Chem., December 13, 1996; 271(50): 32174 - 32179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Funk, I. Adamska, B. R. Green, B. Andersson, and G. Renger The Nuclear-encoded Chlorophyll-binding Photosystem II-S Protein Is Stable in the Absence of Pigments J. Biol. Chem., December 15, 1995; 270(50): 30141 - 30147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. van Wijk, S. Bingsmark, E.-M. Aro, and B. Andersson In Vitro Synthesis and Assembly of Photosystem II Core Proteins J. Biol. Chem., October 27, 1995; 270(43): 25685 - 25695. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
